Detailed information of TA system TA162316

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/HicA-HicB
• Location: 1311070..1311733
• Replicon: chromosome
• Accession: NZ_CP055302
• Organism: Mannheimia pernigra strain 16CN0041

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: -
• Locus tag: HV561_RS06730
• Protein ID: WP_176808235.1
• Coordinates: 1311551..1311733 (-)
• Length: 61 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: HV561_RS06725
• Protein ID: WP_176808234.1
• Coordinates: 1311070..1311522 (-)
• Length: 151 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HV561_RS06685	1306186..1306872	-	687	WP_176808272.1	S24 family peptidase	-
HV561_RS06690	1307001..1307234	+	234	WP_032848913.1	antirepressor protein Cro	-
HV561_RS06695	1307303..1308238	+	936	Protein_1271	DNA helicase	-
HV561_RS06700	1308245..1308703	+	459	Protein_1272	replication protein	-
HV561_RS06705	1308700..1309272	+	573	WP_176808230.1	adenine methyltransferase	-
HV561_RS06710	1309391..1309843	+	453	WP_176808231.1	DUF1367 family protein	-
HV561_RS06715	1309852..1310052	+	201	WP_176808232.1	hypothetical protein	-
HV561_RS06720	1310443..1310763	-	321	Protein_1276	phage repressor protein	-
HV561_RS06725	1311070..1311522	-	453	WP_176808234.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
HV561_RS06730	1311551..1311733	-	183	WP_176808235.1	type II toxin-antitoxin system HicA family toxin	Toxin
HV561_RS06735	1311854..1312423	+	570	WP_176808236.1	recombination protein NinG	-
HV561_RS06740	1312413..1312886	+	474	WP_176808237.1	hypothetical protein	-
HV561_RS06755	1313173..1313748	-	576	WP_176808238.1	hypothetical protein	-
HV561_RS06760	1313861..1314106	+	246	WP_176808239.1	hypothetical protein	-
HV561_RS06765	1314103..1314642	+	540	WP_176808240.1	lysozyme	-
HV561_RS06770	1314615..1314965	+	351	WP_176808241.1	DUF2570 domain-containing protein	-
HV561_RS06775	1314970..1315143	+	174	WP_176808242.1	hypothetical protein	-
HV561_RS06785	1315544..1316068	+	525	WP_176808244.1	terminase small subunit	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	1235768..1361333	125565

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (3-60)

Antitoxin

• HicB-like antitoxin of toxin-antitoxin system (12-138)

Sequences

Toxin

Protein

• Length: 61 a.a.
• Molecular weight: 6728.94 Da
• Isoelectric Point: 10.4193

>T162316 WP_176808235.1 NZ_CP055302:c1311733-1311551 [Mannheimia pernigra]
MHSRDLIKELKAHGCYLKRMGKGDHQIWYSPITGKTFPVPHPKEDLAIGTLKSIKKSAGL

Nucleotide

• Length: 183 bp

>T162316 NZ_CP071731:c1298140-1297850 [Pseudomonas aeruginosa]
ATGACCGAGCAAATTTATGATTATGACCCCGCGCAAGCGCTGGACAGCCCGGAGGCCATGGCTCTGTTCATCGCAGACGC
TTTGGATACTGGAGACACCGCCTATATCGCTAAGGCTATGGGAGTCGTGGCCCGCGCCAAGGGGATGACGGAGTTGGCCA
AGGAAACGGGTTTGGCCAGGGAGCAGCTTTACAAATCGTTTAGTGAGCGCGGGAACCCTACATTGAAAACCATGCTGGCA
GTAATGCGAGCTCTGGGAGTTGATCTGACCGCCCGCCCGCATGCTCAGTGA

Antitoxin

Protein

• Length: 151 a.a.
• Molecular weight: 17113.38 Da
• Isoelectric Point: 4.5217

>AT162316 WP_176808234.1 NZ_CP055302:c1311522-1311070 [Mannheimia pernigra]
MIFTVGLEIPEDTETTAYGMIVPALCEHNYGCFSAADEVEELLPMVTEAIQLNLESMAEDGFDLTTLKDKGIPHYKNDPE
YAHFKDWFLVDVDISPYLGSKQRINITMPEFLLNRIDRRVAAMGNFYKDRSHFLATAAHRELHAHSNTEM

Nucleotide

• Length: 453 bp

>AT162316 NZ_CP071731:c1298436-1298137 [Pseudomonas aeruginosa]
ATGGTCGAAGTCAAACAAACCGCCACTTTCATGGCATGGGAAAGCAAGCTAAAGGATCGGCGGGCAAAGGCCGTGATCGC
CGCCCGTATCTTCCGATTGGCGAATGGACTACCCGGCGATGTGTCACCAGTCGGCCAGGGTGTGAGCGAGTTGAGGATTC
ACTATGGCCCAGGCTATCGGGTGTACTTCCAGCAGCGCGGGACGGAAATCGTCATCCTGCTATGTGGTGGAGACAAGAGC
AGCCAGGCCAGGGATATAGAAATGGCGAAACGCCTGGCAAATGAGTGGAGGCCGCAATGA