Detailed information of TA system TA162310

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relB-parE/ParE-RHH
• Location: 6320..6900
• Replicon: plasmid pKP55_5
• Accession: NZ_CP055299
• Organism: Klebsiella pneumoniae strain KP55

Toxin (Protein)

• Gene name: parE
• Uniprot ID: A0A8J3DTL7
• Locus tag: HUW33_RS29610
• Protein ID: WP_071177730.1
• Coordinates: 6320..6634 (-)
• Length: 105 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: A0A2X1PRM1
• Locus tag: HUW33_RS29615
• Protein ID: WP_000093040.1
• Coordinates: 6622..6900 (-)
• Length: 93 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HUW33_RS29585 (HUW33_29580)	2264..4228	+	1965	WP_071177727.1	TraM recognition domain-containing protein	-
HUW33_RS29590 (HUW33_29585)	4228..4959	+	732	WP_071177728.1	MobC family replication-relaxation protein	-
HUW33_RS29595 (HUW33_29590)	4966..5496	+	531	WP_071177729.1	hypothetical protein	-
HUW33_RS29600 (HUW33_29595)	5523..5702	-	180	WP_000165970.1	Rop family plasmid primer RNA-binding protein	-
HUW33_RS29605 (HUW33_29600)	5728..6156	-	429	WP_001140599.1	hypothetical protein	-
HUW33_RS29610 (HUW33_29605)	6320..6634	-	315	WP_071177730.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
HUW33_RS29615 (HUW33_29610)	6622..6900	-	279	WP_000093040.1	CopG family ribbon-helix-helix protein	Antitoxin
HUW33_RS30015	7075..7440	-	366	WP_072354022.1	TonB family protein	-
HUW33_RS29625 (HUW33_29620)	7437..7808	-	372	WP_001237044.1	cell envelope integrity protein TolA	-
HUW33_RS29630 (HUW33_29625)	8082..8327	-	246	WP_032440458.1	hypothetical protein	-
HUW33_RS29635 (HUW33_29630)	8972..10459	+	1488	WP_004178082.1	group II intron reverse transcriptase/maturase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..11970	11970

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (4-96)

Antitoxin

• Ribbon-helix-helix protein, CopG (5-42)

Sequences

Toxin

Protein

• Length: 105 a.a.
• Molecular weight: 11805.73 Da
• Isoelectric Point: 9.8324

>T162310 WP_071177730.1 NZ_CP055299:c6634-6320 [Klebsiella pneumoniae]
MPQVTISALAQRDLQRLQDFLKTKNRLAARKGGEVIVRAIQQLKTLPDIGRPVPFLPLEYKELVIGFGDSGYVMLYRHDR
EMDQIVIVTVRHQKESGYPGADSL

Nucleotide

• Length: 315 bp

>T162310 NZ_CP071730:c1582424-1582146 [Pseudomonas aeruginosa]
ATGATTCTGACCTTTCGCTGCGACGAGACTCGTCAGCTTTTTGAGACGGGTCTTTCGAGGCGGTGGGGAGCGATCCTCAC
AGTCGCTACGCGTAAGCTCGCAATGCTTCATGCGGCTAGGGAGCTTCGAGACCTGCGCTCTCCACCTGGAAACCGGTTGG
AGCCGTTGCAGGGAAAGCGGGCGGGCCAACATAGCATCAGGATCAATGACCAGTGGCGTGTCTGTTTCGTCTGGACGGAT
GCGGGTCCCGAAGAAGTCGAAATAGTTGATTACCACTGA

Antitoxin

Protein

• Length: 93 a.a.
• Molecular weight: 10709.73 Da
• Isoelectric Point: 4.7561

>AT162310 WP_000093040.1 NZ_CP055299:c6900-6622 [Klebsiella pneumoniae]
MSSATSIRLDEELKDRLKTLADDRHRSAHALMLEAITEYIDREEKRSQYLRDGQAAWQHYQETGLHLTAEEAEAWISTWG
TENEQDAPPCHR

Nucleotide

• Length: 279 bp

>AT162310 NZ_CP071730:c1582134-1581829 [Pseudomonas aeruginosa]
ATGGCTACCAATGGTATGCGCCCCATCCATCCTGGGGAAATATTGCGCGATGAGTTTCTGATGGAGTTTGATATCTCTCC
AGCTGCTCTAGCACGCGCTTTGAAAGTCTCCGCTCCGACAGTGAACGATATCGTTCGTGAGCAGCGTGGTATCTCCGCAG
ATATGGCGATTCGTCTGGGGCGTTACTTTGATACGTCCGCTCAGTTCTGGATGAATCTCCAGAGTGAGTATTCGTTAGCA
ACTGCTTATGCGGCGAATGGCAAGCAGATCGAACATGAGATTGAGCCGCTGCTTGCTCACGGATAG

Structures

Antitoxin

• AlphaFold DB: A0A2X1PRM1