Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) PumA-relB/upstrm_HI1419-dnstrm_HI1420
Location 22722..23304 Replicon plasmid pKP55_4
Accession NZ_CP055298
Organism Klebsiella pneumoniae strain KP55

Toxin (Protein)


Gene name PumA Uniprot ID B1VJ82
Locus tag HUW33_RS29490 Protein ID WP_012368903.1
Coordinates 22722..23018 (+) Length 99 a.a.

Antitoxin (Protein)


Gene name relB Uniprot ID B1VJ81
Locus tag HUW33_RS29495 Protein ID WP_012368902.1
Coordinates 23020..23304 (+) Length 95 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HUW33_RS29455 (HUW33_29450) 17821..18798 - 978 WP_212656691.1 replication initiator protein A -
HUW33_RS29460 (HUW33_29455) 19780..20304 + 525 WP_212656692.1 J domain-containing protein -
HUW33_RS29465 (HUW33_29460) 20558..21109 + 552 WP_212656693.1 hypothetical protein -
HUW33_RS29470 (HUW33_29465) 21131..21397 + 267 WP_212656694.1 hypothetical protein -
HUW33_RS29475 (HUW33_29470) 21387..21617 + 231 WP_073546961.1 hypothetical protein -
HUW33_RS29480 (HUW33_29475) 21990..22202 + 213 WP_073546991.1 hypothetical protein -
HUW33_RS29485 (HUW33_29480) 22249..22392 + 144 WP_212656695.1 hypothetical protein -
HUW33_RS30090 22468..22602 + 135 WP_259493503.1 hypothetical protein -
HUW33_RS29490 (HUW33_29485) 22722..23018 + 297 WP_012368903.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
HUW33_RS29495 (HUW33_29490) 23020..23304 + 285 WP_012368902.1 putative addiction module antidote protein Antitoxin
HUW33_RS29500 (HUW33_29495) 24012..24560 + 549 WP_212656696.1 TraY domain protein -
HUW33_RS29505 (HUW33_29500) 24568..25728 + 1161 WP_212656697.1 MobP1 family relaxase -
HUW33_RS29510 (HUW33_29505) 25738..26094 + 357 WP_212656698.1 DNA distortion polypeptide 3 -
HUW33_RS29515 (HUW33_29510) 26071..26595 - 525 WP_212656699.1 restriction endonuclease -
HUW33_RS29520 (HUW33_29515) 26687..27016 - 330 WP_113355598.1 hypothetical protein -
HUW33_RS29525 (HUW33_29520) 27016..27261 - 246 WP_113355596.1 hypothetical protein -
HUW33_RS29530 (HUW33_29525) 27561..27842 - 282 WP_113355595.1 CopG family transcriptional regulator -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - - 1..34012 34012


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Addiction module killer protein, predicted

(3-96)

Antitoxin

Uncharacterized protein HI1420

(3-90)


Sequences

Toxin        


Download         Length: 99 a.a.        Molecular weight: 11325.21 Da        Isoelectric Point: 10.2910

>T162309 WP_012368903.1 NZ_CP055298:22722-23018 [Klebsiella pneumoniae]
MIEIRQTEEVKKWLKGLKDKIAKAKILTRIERMKEGNYGDVEPIGDGYSELRIHQGKGYRVYFANRNNEIILLLCGGDKT
TQQADIKKAKQIAKKWGF

Download         Length: 297 bp

>T162309 NZ_CP071730:c1298445-1298146 [Pseudomonas aeruginosa]
ATGGTCGAAGTCAAACAAACCGCCACTTTCATGGCATGGGAAAGCAAGCTAAAGGATCGGCGGGCAAAGGCCGTGATCGC
CGCCCGTATCTTCCGATTGGCGAATGGACTACCCGGCGATGTGTCACCAGTCGGCCAGGGTGTGAGCGAGTTGAGGATTC
ACTATGGCCCAGGCTATCGGGTGTACTTCCAGCAGCGCGGGACGGAAATCGTCATCCTGCTATGTGGTGGAGACAAGAGC
AGCCAGGCCAGGGATATAGAAATGGCGAAACGCCTGGCAAATGAGTGGAGGCCGCAATGA

Antitoxin


Download         Length: 95 a.a.        Molecular weight: 10302.61 Da        Isoelectric Point: 4.6286

>AT162309 WP_012368902.1 NZ_CP055298:23020-23304 [Klebsiella pneumoniae]
MKTKPELFDVVDYLEDEEDIQAYLNAALDEDDESYFLAALGNIARARNISQLSKETGMSREGIYKALSGTGNPSFKTISK
ISKALGLKIHFAGA

Download         Length: 285 bp

>AT162309 NZ_CP071730:c1298149-1297859 [Pseudomonas aeruginosa]
ATGACCGAGCAAATTTATGATTATGACCCCGCGCAAGCGCTGGACAGCCCGGAGGCCATGGCTCTGTTCATCGCAGACGC
TTTGGATACTGGAGACACCGCCTATATCGCTAAGGCTATGGGAGTCGTGGCCCGCGCCAAGGGGATGACGGAGTTGGCCA
AGGAAACGGGTTTGGCCAGGGAGCAGCTTTACAAATCGTTTAGTGAGCGCGGGAACCCTACATTGAAAACCATGCTGGCA
GTAATGCGAGCTCTGGGAGTTGATCTGACCGCCCGCCCGCATGCTCAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A1I3XCB7

Antitoxin

Source ID Structure
AlphaFold DB A0A1I3XCJ1

References