Detailed information of TA system TA162308

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): TsxAB/MqsA(antitoxin)
• Location: 21131..21617
• Replicon: plasmid pKP55_4
• Accession: NZ_CP055298
• Organism: Klebsiella pneumoniae strain KP55

Toxin (Protein)

• Gene name: TsxA
• Uniprot ID: -
• Locus tag: HUW33_RS29470
• Protein ID: WP_212656694.1
• Coordinates: 21131..21397 (+)
• Length: 89 a.a.

Antitoxin (Protein)

• Gene name: TsxB
• Uniprot ID: -
• Locus tag: HUW33_RS29475
• Protein ID: WP_073546961.1
• Coordinates: 21387..21617 (+)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HUW33_RS29440 (HUW33_29435)	16809..17048	-	240	WP_113355614.1	hypothetical protein	-
HUW33_RS29445 (HUW33_29440)	17119..17334	-	216	WP_212656689.1	hypothetical protein	-
HUW33_RS29450 (HUW33_29445)	17401..17811	-	411	WP_212656690.1	hypothetical protein	-
HUW33_RS29455 (HUW33_29450)	17821..18798	-	978	WP_212656691.1	replication initiator protein A	-
HUW33_RS29460 (HUW33_29455)	19780..20304	+	525	WP_212656692.1	J domain-containing protein	-
HUW33_RS29465 (HUW33_29460)	20558..21109	+	552	WP_212656693.1	hypothetical protein	-
HUW33_RS29470 (HUW33_29465)	21131..21397	+	267	WP_212656694.1	hypothetical protein	Toxin
HUW33_RS29475 (HUW33_29470)	21387..21617	+	231	WP_073546961.1	hypothetical protein	Antitoxin
HUW33_RS29480 (HUW33_29475)	21990..22202	+	213	WP_073546991.1	hypothetical protein	-
HUW33_RS29485 (HUW33_29480)	22249..22392	+	144	WP_212656695.1	hypothetical protein	-
HUW33_RS30090	22468..22602	+	135	WP_259493503.1	hypothetical protein	-
HUW33_RS29490 (HUW33_29485)	22722..23018	+	297	WP_012368903.1	type II toxin-antitoxin system RelE/ParE family toxin	-
HUW33_RS29495 (HUW33_29490)	23020..23304	+	285	WP_012368902.1	putative addiction module antidote protein	-
HUW33_RS29500 (HUW33_29495)	24012..24560	+	549	WP_212656696.1	TraY domain protein	-
HUW33_RS29505 (HUW33_29500)	24568..25728	+	1161	WP_212656697.1	MobP1 family relaxase	-
HUW33_RS29510 (HUW33_29505)	25738..26094	+	357	WP_212656698.1	DNA distortion polypeptide 3	-
HUW33_RS29515 (HUW33_29510)	26071..26595	-	525	WP_212656699.1	restriction endonuclease	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..34012	34012

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (5-35)

Sequences

Toxin

Protein

• Length: 89 a.a.
• Molecular weight: 10213.78 Da
• Isoelectric Point: 10.1699

>T162308 WP_212656694.1 NZ_CP055298:21131-21397 [Klebsiella pneumoniae]
MKTIRKNKGDVTYYLSRDNNDSYRLIKKIKARATHLVKDGHKTTKITLSDLLLTHDQLYNLDYSLNGLRADDKITIELLI
GEFFKNGK

Nucleotide

• Length: 267 bp

>T162308 NZ_CP071730:c1298149-1297859 [Pseudomonas aeruginosa]
ATGACCGAGCAAATTTATGATTATGACCCCGCGCAAGCGCTGGACAGCCCGGAGGCCATGGCTCTGTTCATCGCAGACGC
TTTGGATACTGGAGACACCGCCTATATCGCTAAGGCTATGGGAGTCGTGGCCCGCGCCAAGGGGATGACGGAGTTGGCCA
AGGAAACGGGTTTGGCCAGGGAGCAGCTTTACAAATCGTTTAGTGAGCGCGGGAACCCTACATTGAAAACCATGCTGGCA
GTAATGCGAGCTCTGGGAGTTGATCTGACCGCCCGCCCGCATGCTCAGTGA

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8772.96 Da
• Isoelectric Point: 8.5162

>AT162308 WP_073546961.1 NZ_CP055298:21387-21617 [Klebsiella pneumoniae]
MENNAKNIKALRLKTGLTQKEMAERYGVGIRTWQKKEEDGTQSSQKLSNFDFEYLLLLAGEHPDFVLEKRNKEVAS

Nucleotide

• Length: 231 bp

>AT162308 NZ_CP071730:c1298445-1298146 [Pseudomonas aeruginosa]
ATGGTCGAAGTCAAACAAACCGCCACTTTCATGGCATGGGAAAGCAAGCTAAAGGATCGGCGGGCAAAGGCCGTGATCGC
CGCCCGTATCTTCCGATTGGCGAATGGACTACCCGGCGATGTGTCACCAGTCGGCCAGGGTGTGAGCGAGTTGAGGATTC
ACTATGGCCCAGGCTATCGGGTGTACTTCCAGCAGCGCGGGACGGAAATCGTCATCCTGCTATGTGGTGGAGACAAGAGC
AGCCAGGCCAGGGATATAGAAATGGCGAAACGCCTGGCAAATGAGTGGAGGCCGCAATGA