Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 5384453..5385078 Replicon chromosome
Accession NZ_CP055294
Organism Klebsiella pneumoniae strain KP55

Toxin (Protein)


Gene name vapC Uniprot ID R4Y4A3
Locus tag HUW33_RS26565 Protein ID WP_002882817.1
Coordinates 5384453..5384836 (-) Length 128 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID J2DFR0
Locus tag HUW33_RS26570 Protein ID WP_004150355.1
Coordinates 5384836..5385078 (-) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HUW33_RS26550 (HUW33_26535) 5381819..5382721 + 903 WP_002882822.1 formate dehydrogenase subunit beta -
HUW33_RS26555 (HUW33_26540) 5382718..5383353 + 636 WP_002882818.1 formate dehydrogenase cytochrome b556 subunit -
HUW33_RS26560 (HUW33_26545) 5383350..5384279 + 930 WP_004150358.1 formate dehydrogenase accessory protein FdhE -
HUW33_RS26565 (HUW33_26550) 5384453..5384836 - 384 WP_002882817.1 type II toxin-antitoxin system VapC family toxin Toxin
HUW33_RS26570 (HUW33_26555) 5384836..5385078 - 243 WP_004150355.1 CopG family transcriptional regulator Antitoxin
HUW33_RS26575 (HUW33_26560) 5385283..5386200 + 918 WP_002882812.1 alpha/beta hydrolase -
HUW33_RS26580 (HUW33_26565) 5386214..5387155 - 942 WP_004178031.1 fatty acid biosynthesis protein FabY -
HUW33_RS26585 (HUW33_26570) 5387200..5387637 - 438 WP_002882809.1 D-aminoacyl-tRNA deacylase -
HUW33_RS26590 (HUW33_26575) 5387634..5388494 - 861 WP_002882807.1 virulence factor BrkB family protein -
HUW33_RS26595 (HUW33_26580) 5388488..5389087 - 600 WP_004151865.1 glucose-1-phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-112)

Antitoxin

Predicted DNA-binding protein, ribbon-helix-helix domain

(16-47)


Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 14377.62 Da        Isoelectric Point: 7.3178

>T162298 WP_002882817.1 NZ_CP055294:c5384836-5384453 [Klebsiella pneumoniae]
MTSGSALFDTNILIDLFSGRREAKQALEAWPPQNAISLITWMEVMVGAKKYHQEQRTRMALSTFNIINISQDIAERSVAL
RQEYKLKLPDAIILATAQLHRLELITRNTKDFAGIPGVVTPYEIHPE

Download         Length: 384 bp

>T162298 NZ_CP071717:1221816-1222079 [Acaryochloris sp. 'Moss Beach']
ATGAGTAGTCGCAAATTGGCCTGGACAGATGCTGCCTGGAACGACTATCTCTACTGGCAAGGACAGGATAAAAAAACGCT
GAAGCGGATCAATAAGCTCATTCAGGCTACGAACAATCAGCCATTTAAAGGCATTGGTAAACCTGAGGCTTTACGAGAAA
ATCTATCGGGATTTTGGTCTCGACGCATAGATGAGACCCATCGATTAGTCTATGCCATTGATGATCACTATCTGACAATT
ATTGCCTGTCGGTATCATTATTAG

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9567.83 Da        Isoelectric Point: 4.3509

>AT162298 WP_004150355.1 NZ_CP055294:c5385078-5384836 [Klebsiella pneumoniae]
MMAERDMGRILLDLSDDVIQRLDDLKVQRNIPRAELLREAVEQYLEKQDRAKDTISSALGLWQDCEEDGMEYQRQLRKEW

Download         Length: 243 bp

>AT162298 NZ_CP071717:1221569-1221823 [Acaryochloris sp. 'Moss Beach']
ATGAACATCATTTCCTTCAGTGAAGCCCGCAGCAAACTGAAAGCTGTCTTCGATCGAGTCGTTGATGATGCCGACTACAC
GATCATCACTCGACGAGATGCTGATGATGCAGTGTTGATGTCTCTGGAGTCTTTTAATAGCTTGCTAGAAACCGTTCATT
TGTTAAAGTCACCCGCCAATGCAGCTCACCTAGAACAATCCATTGCCCAATATCGACAGGGACAAGTGACTGAGCAAGAC
CTCTTGGATGAGTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GPK8

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GGU9

References