Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) kacAT/DUF1778(antitoxin)
Location 4810435..4811245 Replicon chromosome
Accession NZ_CP055294
Organism Klebsiella pneumoniae strain KP55

Toxin (Protein)


Gene name KacT Uniprot ID A0A0H3GMP0
Locus tag HUW33_RS23835 Protein ID WP_002887280.1
Coordinates 4810435..4810968 (-) Length 178 a.a.

Antitoxin (Protein)


Gene name KacA Uniprot ID J2E9Q7
Locus tag HUW33_RS23840 Protein ID WP_002887278.1
Coordinates 4810979..4811245 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HUW33_RS23830 (HUW33_23825) 4809266..4810387 + 1122 WP_002887282.1 cupin domain-containing protein -
HUW33_RS23835 (HUW33_23830) 4810435..4810968 - 534 WP_002887280.1 type II toxin-antitoxin system toxin KacT Toxin
HUW33_RS23840 (HUW33_23835) 4810979..4811245 - 267 WP_002887278.1 type II toxin-antitoxin system antitoxin KacA Antitoxin
HUW33_RS23845 (HUW33_23840) 4811348..4812781 - 1434 WP_002887275.1 Cu(+)/Ag(+) sensor histidine kinase -
HUW33_RS23850 (HUW33_23845) 4812771..4813454 - 684 WP_002887273.1 copper response regulator transcription factor CusR -
HUW33_RS23855 (HUW33_23850) 4813626..4815011 + 1386 WP_002887267.1 efflux transporter outer membrane subunit -
HUW33_RS23860 (HUW33_23855) 4815029..4815373 + 345 WP_002887266.1 cation efflux system protein CusF -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(91-151)

Antitoxin

Vibrio phage ICP1, Orf50

(2-86)


Sequences

Toxin        


Download         Length: 178 a.a.        Molecular weight: 19838.70 Da        Isoelectric Point: 5.2614

>T162295 WP_002887280.1 NZ_CP055294:c4810968-4810435 [Klebsiella pneumoniae]
MEQQLTIEMIADAFSYDITGFDCGEEALNTFLKEHLKRQHDGQILRGYALVSGDTVPRLLGYYTLSGSCFERGMLPSKTQ
QKKIPYQNAPSVTLGRLAIDKSVQGQGWGEMLVAHVMRVVWGASKAVGIYGLFVEALNEKAKAFYLRLGFIQLVDENSNL
LFYPTKSIEQLFTDDES

Download         Length: 534 bp

>T162295 NZ_CP071717:c342903-342589 [Acaryochloris sp. 'Moss Beach']
TTGACCTACTTCTGTCAGGCAATCAGGGTACTTGCTCTCCCTCCAGGTTTGCTAGCTAGATATTTGAGGCTCACCGACCT
CATGCTAGAGTTTGGTCCCAATCTGGGGATGCCTCACACCCGATCAATGGGCGATCGCCTATTTGAGCTGAGGGTCAAGA
GCAAAGAAGGAATTGCGAGAGTGTTTTATTGCACCCTGGTGGGTGAAAAAATAGTGATGCTGCATAGCTTTGTGAAGAAG
ACTCAAAAGACTCCCAAGCAAGAACTGCAGATCGCCCGTCGAAGACTAAAAGAGGTGTTGAAACATGACCCATGA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 9996.46 Da        Isoelectric Point: 10.1757

>AT162295 WP_002887278.1 NZ_CP055294:c4811245-4810979 [Klebsiella pneumoniae]
MPALKKQRIDLRLTDDDKSIIEEAAAISNQTITQFVVASASERAAEVIEQHRRMVLNEQSWSLVMEAITQPPAPNDRLKR
AAKRLQTR

Download         Length: 267 bp

>AT162295 NZ_CP071717:c342599-342309 [Acaryochloris sp. 'Moss Beach']
ATGACCCATGAAGAACTAAAGGCCAAAGCCTTAGAACAACTCGAAGTCGCTGAGGAATATAACAAGTTAGAAGAAGAGTT
TAGTCTGTTAAGACAAATGCTTGCTGCCCGTCAGCAAGCAGGTATGACCCAAGCGGATATTGCCCAAAAAATGGGGACTA
AGGCAACAGCGGTGACTCGACTAGAATCATCTTTGAGTAGTGGGAAGCATTCGCCGTCGCTTTCCACTTTGAGAAAATAT
GCCAAGGCTGTGGGTTGTCGTTTAGAAGTGACGTTAATTCCAGAACAATAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 5ZGN
PDB 5XUN

Antitoxin

Source ID Structure
PDB 5ZGN
AlphaFold DB A0A0H3GLZ1

References