Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 4196122..4196741 Replicon chromosome
Accession NZ_CP055294
Organism Klebsiella pneumoniae strain KP55

Toxin (Protein)


Gene name Hha Uniprot ID R8WYV2
Locus tag HUW33_RS20880 Protein ID WP_002892050.1
Coordinates 4196523..4196741 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID J2DPF6
Locus tag HUW33_RS20875 Protein ID WP_002892066.1
Coordinates 4196122..4196496 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HUW33_RS20865 (HUW33_20855) 4191274..4192467 + 1194 WP_002892072.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
HUW33_RS20870 (HUW33_20860) 4192490..4195636 + 3147 WP_020326861.1 multidrug efflux RND transporter permease subunit AcrB -
HUW33_RS20875 (HUW33_20865) 4196122..4196496 + 375 WP_002892066.1 Hha toxicity modulator TomB Antitoxin
HUW33_RS20880 (HUW33_20870) 4196523..4196741 + 219 WP_002892050.1 HHA domain-containing protein Toxin
HUW33_RS20885 (HUW33_20875) 4196900..4197466 + 567 WP_002892030.1 maltose O-acetyltransferase -
HUW33_RS20890 (HUW33_20880) 4197438..4197578 - 141 WP_004147370.1 hypothetical protein -
HUW33_RS20895 (HUW33_20885) 4197599..4198069 + 471 WP_002892026.1 YlaC family protein -
HUW33_RS20900 (HUW33_20890) 4198044..4199495 - 1452 WP_002892023.1 PLP-dependent aminotransferase family protein -
HUW33_RS20905 (HUW33_20895) 4199596..4200294 + 699 WP_002892021.1 GNAT family protein -
HUW33_RS20910 (HUW33_20900) 4200291..4200431 - 141 WP_002892018.1 type B 50S ribosomal protein L36 -
HUW33_RS20915 (HUW33_20905) 4200431..4200694 - 264 WP_002892011.1 type B 50S ribosomal protein L31 -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-116)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T162294 WP_002892050.1 NZ_CP055294:4196523-4196741 [Klebsiella pneumoniae]
MSDKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPTSVWKFIR

Download         Length: 219 bp

>T162294 NZ_CP071717:c129593-129240 [Acaryochloris sp. 'Moss Beach']
ATGAAGATTTGGGTAGATGCTCAACTTCCACCCACTTTAGCTGAGTGGTTGTCAGCGACCTTTGATGATATCGAAGCTGC
TGCCCTGCGAGATTTGTCCCTACGAGATGCTCAAGATATTGAAATTTTTGAAGCTGCACGGACTGAAAATGCTGTGATTA
TGACGAAGGATAGCGATTTCATTGATTTAGTAGATCGTTTAGGAACGCCTCCTCAGGTCATATGGCTAACCTGTGGGAAT
GTCACAAATCGCAGTTTACAGCAATTGCTGATGACTGCTTTACCTAATGCTTTAGAACAGCTAAGACAGGGAGATAGGAT
TGTGGAAGTCAGTAAAACTTTTGAAATTACTTAG

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14368.06 Da        Isoelectric Point: 4.8989

>AT162294 WP_002892066.1 NZ_CP055294:4196122-4196496 [Klebsiella pneumoniae]
MDEYSPKRHDIAQLRFLCETLYHDCLANLEESNHGWVNDPTSAVNLQLNELIEHIATFALNYKIKYAEDNKLIAQVDEYL
DDTFTLFSNYGINSTDLQKWKKSGNRLFRCFVNASRENPASLSC

Download         Length: 375 bp

>AT162294 NZ_CP071717:c129820-129590 [Acaryochloris sp. 'Moss Beach']
ATGAATGCCACTAACCTACTGACTCGCATTACCCAAGTACCGGGCCAGTGTGGGGGGCGTCCTTGTATCCGAGGAATGCG
AATTAGAGTCTCCGACATATTGGAAATGTTGGCAGAAAATGTCACAACTGCTGAGATTTTAGAAGACTTTCCTGATTTGG
AGTTGGCAGATATTCAAGCCTGCTTGCTGTTTGCGGCACGACGGACTGACGTTCCTCAACTGACCGCATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2P8K6F2

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GJ93

References