Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 5179850..5180475 Replicon chromosome
Accession NZ_CP055293
Organism Klebsiella pneumoniae strain 200

Toxin (Protein)


Gene name vapC Uniprot ID A0A483GDF6
Locus tag HWA76_RS25235 Protein ID WP_004187928.1
Coordinates 5179850..5180233 (-) Length 128 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID J2DFR0
Locus tag HWA76_RS25240 Protein ID WP_004150355.1
Coordinates 5180233..5180475 (-) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HWA76_RS25220 (5177216) 5177216..5178118 + 903 WP_002882822.1 formate dehydrogenase subunit beta -
HWA76_RS25225 (5178115) 5178115..5178750 + 636 WP_002882818.1 formate dehydrogenase cytochrome b556 subunit -
HWA76_RS25230 (5178747) 5178747..5179676 + 930 WP_004150358.1 formate dehydrogenase accessory protein FdhE -
HWA76_RS25235 (5179850) 5179850..5180233 - 384 WP_004187928.1 type II toxin-antitoxin system VapC family toxin Toxin
HWA76_RS25240 (5180233) 5180233..5180475 - 243 WP_004150355.1 CopG family transcriptional regulator Antitoxin
HWA76_RS25245 (5180669) 5180669..5181586 + 918 WP_032437488.1 alpha/beta hydrolase -
HWA76_RS25250 (5181600) 5181600..5182541 - 942 WP_004178031.1 fatty acid biosynthesis protein FabY -
HWA76_RS25255 (5182586) 5182586..5183023 - 438 WP_002882809.1 D-aminoacyl-tRNA deacylase -
HWA76_RS25260 (5183020) 5183020..5183880 - 861 WP_004146232.1 virulence factor BrkB family protein -
HWA76_RS25265 (5183874) 5183874..5184473 - 600 WP_004151865.1 glucose-1-phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-110)

Antitoxin

Predicted DNA-binding protein, ribbon-helix-helix domain

(16-47)


Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 14407.64 Da        Isoelectric Point: 7.3178

>T162280 WP_004187928.1 NZ_CP055293:c5180233-5179850 [Klebsiella pneumoniae]
MTSGSALFDTNILIDLFSGRREAKQALEAWPPQNAISLITWMEVMVGAKKYHQEQRTRMALSTFNIINISQDIAERSVAL
RQEYKLKLPDAIILATAQLHRLELITRNTKDFTGIPGVVTPYEIHPE

Download         Length: 384 bp

>T162280 NZ_CP055293:c5180233-5179850 [Klebsiella pneumoniae]
ATGACCTCCGGATCTGCGCTTTTTGATACCAATATTCTTATTGATTTATTTAGCGGGCGTCGCGAAGCCAAACAGGCGCT
GGAGGCCTGGCCACCGCAGAATGCGATCAGTCTGATTACCTGGATGGAGGTGATGGTTGGCGCTAAAAAATATCACCAGG
AGCAGCGCACGCGAATGGCGCTGAGCACCTTTAATATCATTAACATCTCACAGGATATTGCAGAGCGAAGCGTTGCGCTG
CGGCAGGAGTATAAGCTTAAGCTGCCGGATGCCATCATTCTGGCGACGGCGCAACTCCATCGTCTGGAACTAATTACGCG
GAATACGAAGGATTTTACCGGTATTCCTGGCGTAGTCACGCCGTACGAAATCCACCCTGAATAA

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9567.83 Da        Isoelectric Point: 4.3509

>AT162280 WP_004150355.1 NZ_CP055293:c5180475-5180233 [Klebsiella pneumoniae]
MMAERDMGRILLDLSDDVIQRLDDLKVQRNIPRAELLREAVEQYLEKQDRAKDTISSALGLWQDCEEDGMEYQRQLRKEW

Download         Length: 243 bp

>AT162280 NZ_CP055293:c5180475-5180233 [Klebsiella pneumoniae]
ATGATGGCTGAAAGGGATATGGGCAGAATTCTTCTCGATTTATCAGACGATGTCATTCAACGTCTCGATGACCTCAAAGT
GCAGCGTAACATTCCGCGAGCGGAACTGTTACGTGAAGCGGTTGAACAATATCTGGAGAAACAAGATCGAGCTAAAGATA
CCATCTCCAGTGCGCTGGGTCTGTGGCAAGACTGCGAAGAAGACGGCATGGAATATCAACGTCAGCTACGCAAGGAGTGG
TAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A483GDF6

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GGU9

References