Detailed information of TA system TA162279

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mosAT/AbiEii-AbiEi
• Location: 4857433..4859114
• Replicon: chromosome
• Accession: NZ_CP055293
• Organism: Klebsiella pneumoniae strain 200

Toxin (Protein)

• Gene name: mosT
• Uniprot ID: W8V1Q6
• Locus tag: HWA76_RS23750
• Protein ID: WP_002885155.1
• Coordinates: 4857433..4858350 (-)
• Length: 306 a.a.

Antitoxin (Protein)

• Gene name: mosA
• Uniprot ID: A0A2L1C4M5
• Locus tag: HWA76_RS23755
• Protein ID: WP_004192584.1
• Coordinates: 4858356..4859114 (-)
• Length: 253 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HWA76_RS23730 (4852739)	4852739..4854259	+	1521	WP_002885173.1	sugar ABC transporter ATP-binding protein	-
HWA76_RS23735 (4854256)	4854256..4855254	+	999	WP_002885172.1	ribose ABC transporter permease	-
HWA76_RS23740 (4855285)	4855285..4856226	+	942	WP_002885156.1	ABC transporter substrate-binding protein	-
HWA76_RS23745 (4856301)	4856301..4857308	+	1008	WP_004177805.1	carbohydrate kinase family protein	-
HWA76_RS23750 (4857433)	4857433..4858350	-	918	WP_002885155.1	nucleotidyl transferase AbiEii/AbiGii toxin family protein	Toxin
HWA76_RS23755 (4858356)	4858356..4859114	-	759	WP_004192584.1	type IV toxin-antitoxin system AbiEi family antitoxin	Antitoxin
HWA76_RS23760 (4859200)	4859200..4859928	-	729	WP_002885152.1	response regulator transcription factor	-
HWA76_RS23765 (4860201)	4860201..4862348	+	2148	WP_012737173.1	formate dehydrogenase H subunit alpha, selenocysteine-containing	-
HWA76_RS23770 (4862578)	4862578..4863264	+	687	WP_004151733.1	tetratricopeptide repeat protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Nucleotidyl transferase AbiEii toxin, Type IV TA system (14-278)

Antitoxin

• Transcriptional regulator AbiEi antitoxin, N-terminal domain (1-93)

Sequences

Toxin

Protein

• Length: 306 a.a.
• Molecular weight: 34687.15 Da
• Isoelectric Point: 5.4707

>T162279 WP_002885155.1 NZ_CP055293:c4858350-4857433 [Klebsiella pneumoniae]
MDIQAPYYRQVALLMQVLPYVAVEREFALKGGTAINLFIRDFPRLSVDIDLAWVPLESRAIALPHIRDALARIAANLQQQ
AGMSAVLQANRSDEMRVIVTTDSAQIKIEVSPVARGTLYPPQEREVVGAVEDVFGYASLPVVSLPDLYGGKLCAALDRQH
PRDFYDVKLLLDAQELDRPIFNGFIVYLLSHNRPLAEVLNPRWKDIAEPFYREFSGMTFETIALEELTAVPNRMIAALKS
CFTQQDVDFLLSFKRGEPDWRLAPEMRIQDLPAVQWKLRNIHQMPAIKRAESLDKLEKVLAEWRS

Nucleotide

• Length: 918 bp

>T162279 NZ_CP071711:c2661394-2661083 [Escherichia coli]
ATGCTCTTCATCGAAACGGAAATTTTTACTGAAGATGTTCAAAAACTGCTGACCGACGATGAATTCAGTCGTTTTCAGTT
TTTCCTGGCCTTGAATCCTGACTATGGTGAGGTAATACCTGAAACTGGTGGGTTAAGAAAGGTTCGATGGGTTTCAGGAG
GAAAGGGGAAACGTGCTGGTGTCAGGGTTATTTACTTTCATCAGGTTAAACATTATGAGATCAGATTACTGCTTATTTAT
CGTAAGGGTATTAAAGATGATCTGTCTCCCCAGGAAAAAGCAATGCTCCGGTTGTTAAATACGAGGTGGTAA

Antitoxin

Protein

• Length: 253 a.a.
• Molecular weight: 28582.94 Da
• Isoelectric Point: 10.0870

>AT162279 WP_004192584.1 NZ_CP055293:c4859114-4858356 [Klebsiella pneumoniae]
MATKLNWLLQNSAPGDVILQSWLSQHAISPSLAFKYAQSGWLKKRGNGVYARAGREPEWNDAFACLQNQLAAPVYVAGLS
SLVWQGRSHYLQLKQNQCWLSVENKALLPKWFREFPGVEWIVISGKKLPVLDDRYRVTLDIKGKKLIGSAPELAAYELLS
AVPGTLSFNHAAELFQGLVNLNPRKVEYLLSVSQSVQAKRLYLFFASFYEHGWLKRIDSQKIDLGAGKRQIVENGKFNAQ
YQITVPERFQKE

Nucleotide

• Length: 759 bp

>AT162279 NZ_CP071711:c2661082-2660792 [Escherichia coli]
ATGGATAAGGCACTTTTTGAGCGGTTGACTCACAGCATGGCCCAGATGAATGAAATTATTGAAGGAACGCGACAGCCTTC
GCGGACTTTTGAAGTTGATGCTATGAAAATCAAAGAAATACGCCGCGCTTCAGGGTTGTCACAATCTAAATTTGCCGATC
TTATTTCTGTAAGCGTAGACACGTTGCGTAACTGGGAGCAGGGGCGACGTTCACCGACCGGGCCTGCAAAGGCTTTGCTG
CGAGCGATTGCCAATGATCCGCAACATGTGCTGCAAGCACTTAACCGTTAG

Structures

Toxin

• AlphaFold DB: A0A0H3GH11

Antitoxin

• AlphaFold DB: A0A2L1C4M5