Detailed information of TA system TA162277

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): ykfI-yeeU/CbtA-CbeA
• Location: 4602058..4602784
• Replicon: chromosome
• Accession: NZ_CP055293
• Organism: Klebsiella pneumoniae strain 200

Toxin (Protein)

• Gene name: ykfI
• Uniprot ID: -
• Locus tag: HWA76_RS22545
• Protein ID: WP_032437356.1
• Coordinates: 4602058..4602399 (-)
• Length: 114 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: -
• Locus tag: HWA76_RS22550
• Protein ID: WP_032437354.1
• Coordinates: 4602434..4602784 (-)
• Length: 117 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HWA76_RS22515 (4597065)	4597065..4598336	+	1272	Protein_4415	conjugative transfer system coupling protein TraD	-
HWA76_RS22520 (4598299)	4598299..4598565	+	267	WP_004178415.1	hypothetical protein	-
HWA76_RS22525 (4598565)	4598565..4599237	+	673	Protein_4417	DUF4400 domain-containing protein	-
HWA76_RS22530 (4599248)	4599248..4600132	+	885	WP_004192285.1	RES domain-containing protein	-
HWA76_RS22535 (4600331)	4600331..4600519	-	189	Protein_4419	transposase	-
HWA76_RS22540 (4600536)	4600536..4601647	-	1112	Protein_4420	IS3 family transposase	-
HWA76_RS22545 (4602058)	4602058..4602399	-	342	WP_032437356.1	TA system toxin CbtA family protein	Toxin
HWA76_RS22550 (4602434)	4602434..4602784	-	351	WP_032437354.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
HWA76_RS22555 (4602805)	4602805..4603026	-	222	WP_063931680.1	DUF987 domain-containing protein	-
HWA76_RS22560 (4603042)	4603042..4603515	-	474	WP_032437352.1	DNA repair protein RadC	-
HWA76_RS22565 (4603586)	4603586..4604407	-	822	WP_032437350.1	DUF932 domain-containing protein	-
HWA76_RS22570 (4604503)	4604503..4605180	-	678	WP_032437348.1	hypothetical protein	-
HWA76_RS22575 (4605466)	4605466..4606359	-	894	WP_032437346.1	50S ribosome-binding GTPase	-
HWA76_RS22580 (4606457)	4606457..4607608	+	1152	WP_129075233.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	4592410..4630461	38051

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-108)

Antitoxin

• Antitoxin CbeA (16-115)

Sequences

Toxin

Protein

• Length: 114 a.a.
• Molecular weight: 12991.04 Da
• Isoelectric Point: 5.0978

>T162277 WP_032437356.1 NZ_CP055293:c4602399-4602058 [Klebsiella pneumoniae]
MNTLPVINQRAIQLCPSPVTIWQTLLTRLLEQHYGLALNDTPFGDESVIQEHIEAGITLVDAVNFLVEKYELVRIDRWAF
GWLEPSPYLRAEDILRMRRDMGLLRGCNHTAAM

Nucleotide

• Length: 342 bp

>T162277 NZ_CP071711:2365749-2366126 [Escherichia coli]
ATGAAAACATTACCCGATACACACGTACGGGAGGCATCGAGCTGCCCGTCTCCCGTCACCATCTGGCAGACTCTGCTCTC
CCGACTACTGGGCCAGCATTACGGCCTTACACTGAATGACACACCGTTCGCCGATGAACGTGTGATTGAGCAGCATATTG
AAGCTGGCATTTCACTGTGCGATGCAGTGAACTTTCTTGTTGAAAAATACGCACTGGTGCGTACCGACCAGCCGGAATTC
AGTGCCTGTACCCGCTCTCAGTTAATAAACAGCATCGATATCCTCCGGGCTCGCCGGGCGACTGGCCTGATGACCCGCGA
CAATTACCGAACGGTAAATAACATTACCCTGGGTAAGTATCAGGAGGCGAAACGATGA

Antitoxin

Protein

• Length: 117 a.a.
• Molecular weight: 12859.60 Da
• Isoelectric Point: 6.3108

>AT162277 WP_032437354.1 NZ_CP055293:c4602784-4602434 [Klebsiella pneumoniae]
MSNKTQTVNGDTAEPRWGLSCNVIPCFGARLVQEGNRLHYLADRASITGQFNEADLIHPDQAFPVLLKQAELMLTSGELN
PRHQHCVTFCEKGLTCEADTLGSCGHVYIVIYPTQR

Nucleotide

• Length: 351 bp

>AT162277 NZ_CP071711:2365291-2365659 [Escherichia coli]
GTGTCAGACACACTTTCCGGGACAACGCATCCCGATGACAATGACGACCATCCCTGGTGGGGACTACCCTGCACAGTGAC
GCCCTGTTTCGGCGCCCGTCTGGTGCAGGAGGGTAACCGGTTGCATTACCTTGCCGACCGTGCCGGTATCAGAGGCCGGT
TCAGCGATGCAGATGCGTACCACCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAACTCATGCTCACCAGCGGT
GAACTGAATCCCCGCCATCAGCATACCGTCACGCTGTATGCAAAAGGTCTGACCTGCAAAGCTGACACTCTCGGTAGCGG
TGGTTACGTTTATCTGGCTGTTTATCCGACGCCCGAAACGAAAAAGTAA