Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) kacAT/DUF1778(antitoxin)
Location 4563482..4564292 Replicon chromosome
Accession NZ_CP055293
Organism Klebsiella pneumoniae strain 200

Toxin (Protein)


Gene name KacT Uniprot ID A0A483G813
Locus tag HWA76_RS22330 Protein ID WP_023279404.1
Coordinates 4563482..4564015 (-) Length 178 a.a.

Antitoxin (Protein)


Gene name KacA Uniprot ID J2E9Q7
Locus tag HWA76_RS22335 Protein ID WP_002887278.1
Coordinates 4564026..4564292 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HWA76_RS22325 (4562313) 4562313..4563434 + 1122 WP_002887282.1 cupin domain-containing protein -
HWA76_RS22330 (4563482) 4563482..4564015 - 534 WP_023279404.1 type II toxin-antitoxin system toxin KacT Toxin
HWA76_RS22335 (4564026) 4564026..4564292 - 267 WP_002887278.1 type II toxin-antitoxin system antitoxin KacA Antitoxin
HWA76_RS22340 (4564395) 4564395..4565828 - 1434 WP_004192214.1 Cu(+)/Ag(+) sensor histidine kinase -
HWA76_RS22345 (4565818) 4565818..4566501 - 684 WP_002887273.1 copper response regulator transcription factor CusR -
HWA76_RS22350 (4566673) 4566673..4568058 + 1386 WP_004192218.1 efflux transporter outer membrane subunit -
HWA76_RS22355 (4568076) 4568076..4568420 + 345 WP_004192220.1 cation efflux system protein CusF -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(91-151)

Antitoxin

Vibrio phage ICP1, Orf50

(2-86)


Sequences

Toxin        


Download         Length: 178 a.a.        Molecular weight: 19809.71 Da        Isoelectric Point: 6.2369

>T162276 WP_023279404.1 NZ_CP055293:c4564015-4563482 [Klebsiella pneumoniae]
MEQQLTIEMIADAFSYDITGFDCGEEALNTFLKEHLKRQHDGQILRGYALVSGDTVPRLLGYYTLSGSCFERGMLPSKTQ
QKKIPYQNAPSVTLGRLAIDKSVQGQGWGEMLVAHAMRVVWGASKAVGIYGLFVEALNEKAKAFYLRLGFIQLVDKNSNL
LFYPTKSIEQLFTDDES

Download         Length: 534 bp

>T162276 NZ_CP071711:c2217129-2216779 [Escherichia coli]
ATGGTAAAGAAAAGTGAATTTGAACGGGGAGACATTGTGCTGGTTGGCTTTGATCCAGCAAGCGGCCATGAACAGCAAGG
TGCTGGTCGACCTGCGCTTGTGCTCTCCGTTCAAGCCTTTAATCAACTGGGAATGACGCTGGTGGCCCCCATTACGCAGG
GCGGAAATTTTGCCCGTTATGCCGGATTTAGCGTTCCTTTACATTGCGAAGAAGGCGATGTGCACGGCGTGGTGCTGGTG
AATCAGGTGCGGATGATGGATCTACGCGCCCGGCTGGCAAAGCGTATTGGTCTGGCTGCGGATGAGGTGGTGGAAGAGGC
GTTATTACGCTTGCAGGCGGTGGTGGAATAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 9996.46 Da        Isoelectric Point: 10.1757

>AT162276 WP_002887278.1 NZ_CP055293:c4564292-4564026 [Klebsiella pneumoniae]
MPALKKQRIDLRLTDDDKSIIEEAAAISNQTITQFVVASASERAAEVIEQHRRMVLNEQSWSLVMEAITQPPAPNDRLKR
AAKRLQTR

Download         Length: 267 bp

>AT162276 NZ_CP071711:c2217374-2217123 [Escherichia coli]
ATGCGTATTACCATAAAAAGATGGGGGAACAGTGCAGGTATGGTCATTCCCAATATCGTAATGAAAGAACTTAACTTACA
GCCGGGGCAGAGCGTGGAAGCGCAGGTGAGCAACAACCAACTGATTCTGACACCCATCTCCAGGCGCTACTCGCTTGATG
AACTGCTGGCACAGTGTGACATGAACGCCGCGGAACTTAGCGAGCAGGATGTCTGGGGTAAATCCACCCCTGCGGGTGAC
GAAATATGGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A483G813

Antitoxin

Source ID Structure
PDB 5ZGN
AlphaFold DB A0A0H3GLZ1

References