Detailed information of TA system TA162274

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hipBA/HipA-HipB
• Location: 2635940..2637528
• Replicon: chromosome
• Accession: NZ_CP055293
• Organism: Klebsiella pneumoniae strain 200

Toxin (Protein)

• Gene name: hipA
• Uniprot ID: A0A486KW17
• Locus tag: HWA76_RS12970
• Protein ID: WP_004190166.1
• Coordinates: 2635940..2637262 (-)
• Length: 441 a.a.

Antitoxin (Protein)

• Gene name: hipB
• Uniprot ID: A0A0H3GXD8
• Locus tag: HWA76_RS12975
• Protein ID: WP_002904790.1
• Coordinates: 2637262..2637528 (-)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HWA76_RS12955 (2630996)	2630996..2633497	+	2502	WP_004190161.1	fimbria/pilus outer membrane usher protein	-
HWA76_RS12960 (2633485)	2633485..2634489	+	1005	WP_004190163.1	fimbrial protein	-
HWA76_RS12965 (2634631)	2634631..2635923	+	1293	WP_002904794.1	glycoside hydrolase family 10 protein	-
HWA76_RS12970 (2635940)	2635940..2637262	-	1323	WP_004190166.1	type II toxin-antitoxin system HipA family toxin	Toxin
HWA76_RS12975 (2637262)	2637262..2637528	-	267	WP_002904790.1	type II toxin-antitoxin system antitoxin HipB	Antitoxin
HWA76_RS12980 (2637793)	2637793..2638119	-	327	WP_004143476.1	hydroxyisourate hydrolase	-
HWA76_RS12985 (2638116)	2638116..2638616	-	501	WP_004190169.1	2-oxo-4-hydroxy-4-carboxy-5-ureidoimidazoline decarboxylase	-
HWA76_RS12990 (2638594)	2638594..2639973	-	1380	WP_004184021.1	nucleobase:cation symporter-2 family protein	-
HWA76_RS12995 (2640319)	2640319..2641473	+	1155	WP_002904783.1	FAD-dependent urate hydroxylase HpxO	-
HWA76_RS13000 (2641454)	2641454..2642377	-	924	WP_004190172.1	LysR family hpxDE operon transcriptional regulator HpxR	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HipA, N-terminal subdomain 1 (4-103)

Antitoxin

• Cro/C1-type helix-turn-helix domain (16-65)

Sequences

Toxin

Protein

• Length: 441 a.a.
• Molecular weight: 49171.46 Da
• Isoelectric Point: 7.5357

>T162274 WP_004190166.1 NZ_CP055293:c2637262-2635940 [Klebsiella pneumoniae]
MATLTTWMNNVRVGTLTRQANGAHSFRYDEEWLRSPRARPLSLSLPLQYGNITADAVYHYFDNLLPDSPQVRDRIVRRYQ
ARSKQPFDLLAEVGRDSVGAVTLLPPGEEAHLEGLRWQTLDEAQLTTLLTAYQSDIPLGMITSQDDFRISVAGAQEKTAL
LRMGEQWCIPQGATPTTHIIKLPIGEIKQPNATLDLRESVDNEYLCLALARELGLAVPEAEIIATPRIRALAVTRFDRRW
AQEGRVLLRLPQEDLCQAFGLPSAMKYESDGGPGIAAIMTFLLGSSEALKDRYDFMKFMVFQWLTGATDGHAKNFSIYLL
PGGSYRLTPFYDIISAFPVLGGTGLHLRDLKLSMGLNATKGRKTEINAIYPRHFLATAKAVNFPREQMLAILQEFAGHVP
QAIESARRTLPADFSSHVWQAITENMLKLHARLQQGLQAL

Nucleotide

• Length: 1323 bp

>T162274 NZ_CP071711:1757673-1757951 [Escherichia coli]
ATGATTCAAAGGGATATTGAATACTCGGGACAATTTTCAAAGGATGTAAAACTTGCACAAAAGCGTCATAAGGATATGAA
TAAATTGAAATATCTTATGACGCTTCTTATCAATAATGCTTTACCGCTTCCAGCTGTTTATAAAGACCACCCGCTGCAAG
GTTCATGGAAAGGTTATCGCGATGCTCATGTCGAACCGGACTGGATCCTGATTTACAAACTTACCGATAAACTTTTACGA
TTTGAGAGAACTGGAACTCACGCGGCGCTCTTTGGGTAA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 9906.27 Da
• Isoelectric Point: 8.5912

>AT162274 WP_002904790.1 NZ_CP055293:c2637528-2637262 [Klebsiella pneumoniae]
MNSPQIYSPVQLANYLKLLRQQNHWTQDALARRIGIKQATLSNFENHPDKTTLTTLFKILQSLGVVMTLNAKAAETAPAP
DNNTDLDW

Nucleotide

• Length: 267 bp

>AT162274 NZ_CP071711:1757410-1757670 [Escherichia coli]
ATGGCTGCTAACGCATTTGTTCGCGCCCGAATCGATGAAGATCTGAAGAATCAGGCGGCGGACGTACTGGCCGGGATGGG
GCTGACCATCTCTGACCTGGTTCGCATAACCCTCACAAAGGTCGCGCGTGAAAAGGCATTGCCGTTTGATTTACGCGAGC
CTAATCAATTAACCATTCAATCAATCAAAAACAGCGAAGCTGGCGTTGATGTTCATAAGGCCAAAGACGCCGATGATTTA
TTTGATAAATTAGGAATTTAA

Structures

Toxin

• AlphaFold DB: A0A486KW17

Antitoxin

• AlphaFold DB: A0A0H3GXD8