Detailed information of TA system TA162236

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): AbkB-brnA/BrnT-BrnA
• Location: 3770..4358
• Replicon: plasmid pBspH9
• Accession: NZ_CP055291
• Organism: Acinetobacter sp. NEB 394

Toxin (Protein)

• Gene name: AbkB
• Uniprot ID: -
• Locus tag: HUK62_RS18855
• Protein ID: WP_130114512.1
• Coordinates: 4071..4358 (-)
• Length: 96 a.a.

Antitoxin (Protein)

• Gene name: brnA
• Uniprot ID: -
• Locus tag: HUK62_RS18850
• Protein ID: WP_176538642.1
• Coordinates: 3770..4084 (-)
• Length: 105 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HUK62_RS18825	191..772	+	582	WP_086179913.1	phosphoglycerate mutase family protein	-
HUK62_RS18830	849..1721	-	873	WP_176538638.1	hypothetical protein	-
HUK62_RS18835	1714..2814	-	1101	WP_176538639.1	site-specific DNA-methyltransferase	-
HUK62_RS18840	2920..3276	+	357	WP_176538640.1	hypothetical protein	-
HUK62_RS18845	3371..3682	-	312	WP_176538641.1	hypothetical protein	-
HUK62_RS18850	3770..4084	-	315	WP_176538642.1	BrnA antitoxin family protein	Antitoxin
HUK62_RS18855	4071..4358	-	288	WP_130114512.1	BrnT family toxin	Toxin
HUK62_RS18860	4694..5296	-	603	WP_119686775.1	GNAT family N-acetyltransferase	-
HUK62_RS18865	5537..6052	-	516	WP_086179909.1	plasmid replication DNA-binding protein	-
HUK62_RS18870	6131..7060	-	930	WP_176538643.1	replication initiation protein RepM	-
HUK62_RS18875	7925..8302	-	378	WP_086179911.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..8955	8955

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Ribonuclease toxin, BrnT, of type II toxin-antitoxin system (7-88)

Antitoxin

• BrnA antitoxin of type II toxin-antitoxin system (41-96)

Sequences

Toxin

Protein

• Length: 96 a.a.
• Molecular weight: 11262.71 Da
• Isoelectric Point: 6.0900

>T162236 WP_130114512.1 NZ_CP055291:c4358-4071 [Acinetobacter sp. NEB 394]
MKQYFEWDEAKNRKNQKKHDVSFETASLVFEDPLRISIQDRHTDGEERWQTIGKVKGVLMLLVAHTIFDEDDCEIIRIIS
ARQVTKAERDKYEHG

Nucleotide

• Length: 288 bp

>T162236 NZ_CP071693:c4031362-4031054 [Salmonella sp. SAL-045]
ATGCAATACATGGTTTATCGCAATAAAGGAAACAGCAAGGCTTATCCTTATTTACTTGATGTGCAAAGCGATATTATTGA
TGAACTGCATACTCGCATGGTTATCCCATTGTTTCCTGTGAGCAGATTGGTAAATAACCCAGTTAAGCGCCTCACGCCCA
CTCTGAATGTGGAAGGTAATGACTATCTGGTGATGACTCACGAAATGGCGAGTATCAGGCTTTCGCAGATTGGTGATGAG
GTGATGGACGTTCGTTCCCACAGGCAAACCATCAAAAATGCTCTCGATTTTATCTTTGACGGGTTCTGA

Antitoxin

Protein

• Length: 105 a.a.
• Molecular weight: 12214.84 Da
• Isoelectric Point: 7.0149

>AT162236 WP_176538642.1 NZ_CP055291:c4084-3770 [Acinetobacter sp. NEB 394]
MSMVRYSRKELNEKFSDKQDAEIERLLAKGTIPDDQLDLSDIPEVADWSNAVRQNQFYRPVKQQTSIRLDADVLAWFKAQ
GKGYQTRMNEILRDAMLKELKNHQ

Nucleotide

• Length: 315 bp

>AT162236 NZ_CP071693:c4031604-4031365 [Salmonella sp. SAL-045]
ATGCCGGGTATGAGTGTACGACATAAAAAAACAGTCAGTGTCACGCTGGAACCTGCATTGCTTCAGCAAGCCCGTGATGC
AGGTATTAATCTGTCAGCGGTGCTTACCGCCGCGCTGAAAGAGGAAATCAGTGCCACAGGCGTTGAACGCTGGAAAGCAG
AAAATAGAGCGGGTTTGCAGGAGCTCAATCGTATTACTGATGAGCACGGCCTTTTATCGGACGATTACAGGACGTTTTAA