Detailed information of TA system TA162235

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): BrnTA/BrnT-BrnA
• Location: 4668..5256
• Replicon: plasmid pBspH8
• Accession: NZ_CP055290
• Organism: Acinetobacter sp. NEB 394

Toxin (Protein)

• Gene name: brnT
• Uniprot ID: -
• Locus tag: HUK62_RS18790
• Protein ID: WP_176538634.1
• Coordinates: 4668..4955 (+)
• Length: 96 a.a.

Antitoxin (Protein)

• Gene name: brnA
• Uniprot ID: -
• Locus tag: HUK62_RS18795
• Protein ID: WP_176538635.1
• Coordinates: 4942..5256 (+)
• Length: 105 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HUK62_RS18770	461..1219	+	759	WP_130136800.1	hypothetical protein	-
HUK62_RS18775	1936..2871	+	936	WP_055414480.1	replication initiation protein RepM	-
HUK62_RS18780	2956..3489	+	534	WP_130136802.1	plasmid replication DNA-binding protein	-
HUK62_RS18785	3730..4332	+	603	WP_119686775.1	GNAT family N-acetyltransferase	-
HUK62_RS18790	4668..4955	+	288	WP_176538634.1	BrnT family toxin	Toxin
HUK62_RS18795	4942..5256	+	315	WP_176538635.1	BrnA antitoxin family protein	Antitoxin
HUK62_RS18800	5441..5887	+	447	WP_176538636.1	hypothetical protein	-
HUK62_RS18805	6031..8643	-	2613	WP_176538637.1	DEAD/DEAH box helicase family protein	-
HUK62_RS18810	8713..8937	-	225	WP_005155906.1	hypothetical protein	-
HUK62_RS18815	9116..9376	+	261	WP_126039475.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..10333	10333

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Ribonuclease toxin, BrnT, of type II toxin-antitoxin system (7-88)

Antitoxin

• BrnA antitoxin of type II toxin-antitoxin system (41-96)

Sequences

Toxin

Protein

• Length: 96 a.a.
• Molecular weight: 11247.65 Da
• Isoelectric Point: 6.0872

>T162235 WP_176538634.1 NZ_CP055290:4668-4955 [Acinetobacter sp. NEB 394]
MEQYFEWDEAKNRKNQKKHDVSFETASLVFDDPLRISIQNRHTDGEERWQTIGKVKGVLMLLVAHTIFDEDDCEIIRIIS
ARQVTKAERNKYEHG

Nucleotide

• Length: 288 bp

>T162235 NZ_CP071693:c3987370-3987083 [Salmonella sp. SAL-045]
ATGCCGATGGAGTTTGAATGGGATGCGAACAAGGCTAAAAGCAACCTGAGGAAGCACGGTGTCCGCTTTGAGGATGCGGT
ACTGGTGTTTGACGATCCCCGACACCTGTCACGTCAGGAGCGTTATGAGAATGGAGAATATCGTTGGCAGACCCTCGGCC
TTGTTCATGGCATTGTGGTCATTCTGGTTGCTCATAGCGTTCGGTTCGAAAGTGGTTTTGAAGTTATCCGTATCATCAGC
GCTCGCAAGGCAGACAGAAAAGAGAGGAATCGTTATGAGCATGGTTAA

Antitoxin

Protein

• Length: 105 a.a.
• Molecular weight: 12235.91 Da
• Isoelectric Point: 9.4865

>AT162235 WP_176538635.1 NZ_CP055290:4942-5256 [Acinetobacter sp. NEB 394]
MSMVRYSRKELNEKFGEKQDAEIQRLLAKGTVPDDQLNLSDIPEITDWSNAVRHNQFYRPVKQQTSIRLDADVLAWFKAQ
GKGYQTRMNEILRDAMLKELKNHQ

Nucleotide

• Length: 315 bp

>AT162235 NZ_CP071693:c3987096-3986794 [Salmonella sp. SAL-045]
ATGAGCATGGTTAAACATAAACGCGGCAATGCATCCGCGCTGAGTGCTCAGCATGAGGCTGAACTGAAGGCGCTGGCGAA
AAAATCAGATGATGAGATTGATTACAGCGACATTCCCGCTTCGGAGGATGGGCAGTGGTCTGAAGCAGTGCGTGGTAAGT
TTTTTCGCCCGTTAAAAACTCAGGCCTCGGTGCGTATTGACGCCGATGTGATGGAGTGGCTGAAACGGCCAGGAAAAGGC
TACCAGACGCGGCTCAATGCCATTCTGCGCGAGGCGATGCTGCGCGAGCAAAATAAGAAATAG