Detailed information of TA system TA162234

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VagC
• Location: 2461..3083
• Replicon: plasmid pBspH7
• Accession: NZ_CP055289
• Organism: Acinetobacter sp. NEB 394

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: A0A7T4PB98
• Locus tag: HUK62_RS18710
• Protein ID: WP_012268403.1
• Coordinates: 2688..3083 (+)
• Length: 132 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: -
• Locus tag: HUK62_RS18705
• Protein ID: WP_106440603.1
• Coordinates: 2461..2691 (+)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HUK62_RS18695	1..666	+	666	Protein_0	Abi family protein	-
HUK62_RS18700	944..1858	-	915	WP_176538623.1	hypothetical protein	-
HUK62_RS18705	2461..2691	+	231	WP_106440603.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
HUK62_RS18710	2688..3083	+	396	WP_012268403.1	type II toxin-antitoxin system VapC family toxin	Toxin
HUK62_RS18715	3325..4026	-	702	WP_176538624.1	hypothetical protein	-
HUK62_RS18720	4339..4959	+	621	WP_176538625.1	hypothetical protein	-
HUK62_RS18725	5241..5501	+	261	WP_176538626.1	hypothetical protein	-
HUK62_RS18730	5539..5874	+	336	WP_176538627.1	helix-turn-helix domain-containing protein	-
HUK62_RS18735	5878..6435	-	558	WP_176538628.1	plasmid replication DNA-binding protein	-
HUK62_RS18740	6512..7408	-	897	WP_176538629.1	replication initiation protein RepM	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..12103	12103

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (3-124)

Antitoxin

• SpoVT-AbrB domain (7-37)

Sequences

Toxin

Protein

• Length: 132 a.a.
• Molecular weight: 14907.18 Da
• Isoelectric Point: 6.2253

>T162234 WP_012268403.1 NZ_CP055289:2688-3083 [Acinetobacter sp. NEB 394]
MIYLLDTNICIYVINNKPQHVFERFKQYQLGQLAISSITASELAFGVEKSGSERNKQALNKFLSPLEILPYDEQAIWHYA
KLRQDLQSTGKPIGSLDMLIAAHALALDVVLVTNNTKEFERIDGLKLENWV

Nucleotide

• Length: 396 bp

>T162234 NZ_CP071693:3602022-3602279 [Salmonella sp. SAL-045]
ATGAATGCCTCCGGCGTCAGCGTCCGCCACATCAACAGCAAAACCCACATGACCACCTATTACTCACAAATCCCCAGCCT
GCATCTTAAGGGCGACTGGCTGGAAGAAGCGGGATTTAAGACCGGGCGCGGCGTCACCGTGAAGATTTCACAGGGGTGTA
TTGTGCTGATGGCGGACAGTAACGAGGAGCAGAAGCTGCGCGAGCAGCTTTATCAGGCTAAACAGGTGGTTAAGGGAATT
AAGGACGTGCTGGTTTAG

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8793.06 Da
• Isoelectric Point: 4.7708

>AT162234 WP_106440603.1 NZ_CP055289:2461-2691 [Acinetobacter sp. NEB 394]
MEIAKVFQTGRSQAVRLPKAFRFNGTEVAIKSFGRGVLLMPIDNPWDVMLEAINEFEVGFQLERADQGEQFREEFR

Nucleotide

• Length: 231 bp

>AT162234 NZ_CP071693:c3602197-3601865 [Salmonella sp. SAL-045]
TCCGCCATCAGCACAATACACCCCTGTGAAATCTTCACGGTGACGCCGCGCCCGGTCTTAAATCCCGCTTCTTCCAGCCA
GTCGCCCTTAAGATGCAGGCTGGGGATTTGTGAGTAATAGGTGGTCATGTGGGTTTTGCTGTTGATGTGGCGGACGCTGA
CGCCGGAGGCATTCATATTTGCTGACTAAATAAATTCTTAATTATCCGCCGGATGCTGGCTGATTGTGGAGCTCAGGGTG
AGCGAGTATGGGCGCGACATCCTGGCACTGAGCCCCCCTCTCCCCGGCCAGCTCCGGGCGGTGATGAGCAACCGGCTGCC
GGGGCCGTATTTT

Structures

Toxin

• AlphaFold DB: A0A7T4PB98