Detailed information of TA system TA162233

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relE-dinJ/YafQ-RelB
• Location: 7251..7788
• Replicon: plasmid pBspH5
• Accession: NZ_CP055287
• Organism: Acinetobacter sp. NEB 394

Toxin (Protein)

• Gene name: relE
• Uniprot ID: -
• Locus tag: HUK62_RS18575
• Protein ID: WP_127219988.1
• Coordinates: 7251..7541 (-)
• Length: 97 a.a.

Antitoxin (Protein)

• Gene name: dinJ
• Uniprot ID: N9NX23
• Locus tag: HUK62_RS18580
• Protein ID: WP_005254175.1
• Coordinates: 7528..7788 (-)
• Length: 87 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HUK62_RS18545	2975..3919	-	945	WP_176538610.1	restriction endonuclease	-
HUK62_RS18550	3916..5346	-	1431	WP_176538611.1	Eco57I restriction-modification methylase domain-containing protein	-
HUK62_RS18555	5624..5938	-	315	WP_005098515.1	BrnA antitoxin family protein	-
HUK62_RS18560	5925..6212	-	288	WP_005244631.1	BrnT family toxin	-
HUK62_RS18565	6481..6738	-	258	WP_120385287.1	hypothetical protein	-
HUK62_RS18570	6744..6962	-	219	WP_120385288.1	hypothetical protein	-
HUK62_RS18575	7251..7541	-	291	WP_127219988.1	type II toxin-antitoxin system YafQ family toxin	Toxin
HUK62_RS18580	7528..7788	-	261	WP_005254175.1	type II toxin-antitoxin system RelB/DinJ family antitoxin	Antitoxin
HUK62_RS18585	7913..8332	+	420	WP_176538612.1	SMI1/KNR4 family protein	-
HUK62_RS18590	8521..9087	-	567	WP_032024465.1	plasmid replication DNA-binding protein	-
HUK62_RS18595	9151..10074	-	924	WP_032066855.1	replication initiation protein RepM	-
HUK62_RS18600	11250..11636	+	387	WP_032001883.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..13799	13799

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (4-94)

Antitoxin

• RelB antitoxin/Antitoxin DinJ (4-69)

Sequences

Toxin

Protein

• Length: 97 a.a.
• Molecular weight: 11268.05 Da
• Isoelectric Point: 8.8769

>T162233 WP_127219988.1 NZ_CP055287:c7541-7251 [Acinetobacter sp. NEB 394]
MRTIKYTSAFKKDYKREKKGQHRNTVDTELQTVLELLQADEPLPEKYRDHALVSNWVGHRDCHIKPDLVLIYKKTEEGEL
LILKLVRLGSHSELVI

Nucleotide

• Length: 291 bp

>T162233 NZ_CP071693:3411660-3411878 [Salmonella sp. SAL-045]
ATGTCTGATAAACCATTAACTAAAACTGATTATTTGATGCGTTTACGGCGCTGTCAGACAATTGACACTCTGGAGCGCGT
CATTGAGAAAAATAAATATGAATTGTCCGACAATGAGCTGGCTGTATTTTACTCAGCTGCGGATCACCGTCTTGCAGAAT
TGACGATGAACAAACTCTACGACAAGATCCCCTCTTCAGTATGGAAATTCATTCGTTAA

Antitoxin

Protein

• Length: 87 a.a.
• Molecular weight: 9390.76 Da
• Isoelectric Point: 4.4354

>AT162233 WP_005254175.1 NZ_CP055287:c7788-7528 [Acinetobacter sp. NEB 394]
MASNALVTTRINENIKHEATEVLATMGLTVSDAVRLLLTKVAKEKALPFEIWQPNAETIAAMEEDLSKAKSFTSVKDLMA
DLNADD

Nucleotide

• Length: 261 bp

>AT162233 NZ_CP071693:3411258-3411632 [Salmonella sp. SAL-045]
ATGGACGAATACTCACCCAAAAGACATGATATAGCACAGCTTAAATTTCTTTGCGAAACCCTGTATCATGACTGTCTTGC
AAACCTTGAAGAAAGCAACCATGGCTGGGTTAATGACCCGACCTCAGCAGTCAACCTGCAGCTCAATGAGCTGATAGAGC
ATATTGCAACTTTCGCGCTTAATTATAAAATTAAGTACAATGAAGACAATAAATTGATTGCGCAGATAGATGAATACCTG
GACGACACCTTTATGTTGTTCAGCAGCTATGGTATTAATACGCAGGATTTGCAAAAATGGCGTAAATCGGGCAACCGCCT
GTTCCGTTGTTTCGTCAACGCCACCAGGGCCAATCCTGTTAGTTTGTCTTGTTAA

Structures

Antitoxin

• AlphaFold DB: N9NX23