162232

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) BrnTA/BrnT-BrnA
Location 5624..6212 Replicon plasmid pBspH5
Accession NZ_CP055287
Organism Acinetobacter sp. NEB 394

Toxin (Protein)


Gene name brnT Uniprot ID N9P1T1
Locus tag HUK62_RS18560 Protein ID WP_005244631.1
Coordinates 5925..6212 (-) Length 96 a.a.

Antitoxin (Protein)


Gene name brnA Uniprot ID N9PH66
Locus tag HUK62_RS18555 Protein ID WP_005098515.1
Coordinates 5624..5938 (-) Length 105 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HUK62_RS18535 837..1847 - 1011 WP_069119648.1 hypothetical protein -
HUK62_RS18540 1988..2917 - 930 WP_069119653.1 hypothetical protein -
HUK62_RS18545 2975..3919 - 945 WP_176538610.1 restriction endonuclease -
HUK62_RS18550 3916..5346 - 1431 WP_176538611.1 Eco57I restriction-modification methylase domain-containing protein -
HUK62_RS18555 5624..5938 - 315 WP_005098515.1 BrnA antitoxin family protein Antitoxin
HUK62_RS18560 5925..6212 - 288 WP_005244631.1 BrnT family toxin Toxin
HUK62_RS18565 6481..6738 - 258 WP_120385287.1 hypothetical protein -
HUK62_RS18570 6744..6962 - 219 WP_120385288.1 hypothetical protein -
HUK62_RS18575 7251..7541 - 291 WP_127219988.1 type II toxin-antitoxin system YafQ family toxin -
HUK62_RS18580 7528..7788 - 261 WP_005254175.1 type II toxin-antitoxin system RelB/DinJ family antitoxin -
HUK62_RS18585 7913..8332 + 420 WP_176538612.1 SMI1/KNR4 family protein -
HUK62_RS18590 8521..9087 - 567 WP_032024465.1 plasmid replication DNA-binding protein -
HUK62_RS18595 9151..10074 - 924 WP_032066855.1 replication initiation protein RepM -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - - 1..13799 13799


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Ribonuclease toxin, BrnT, of type II toxin-antitoxin system

(7-88)

Antitoxin

BrnA antitoxin of type II toxin-antitoxin system

(41-96)


Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11291.66 Da        Isoelectric Point: 5.2134

>T162232 WP_005244631.1 NZ_CP055287:c6212-5925 [Acinetobacter sp. NEB 394]
MEQYFEWDEAKNRKNQKKHDISFETASLVFDDPLRISIQDRHTDGEERWQTIGKVKGVLMLLVAHTIFDEDDCEIIRIIS
ARQVTKAERDRYEHG

Download         Length: 288 bp

>T162232 NZ_CP071693:c2693154-2693051 [Salmonella sp. SAL-045]
GGCAAGGCGATTTAGCCTGCATTAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTGGACCGAATAC
AGGAATCGTATTCGGTCTCTTTTT

Antitoxin


Download         Length: 105 a.a.        Molecular weight: 12270.93 Da        Isoelectric Point: 8.4842

>AT162232 WP_005098515.1 NZ_CP055287:c5938-5624 [Acinetobacter sp. NEB 394]
MSMVRYSRKELNEKFSDKQDAEIQRLLAKGTVPDDQLDLSDMPEITDWSNAVRHNQFYRPVKQQTSIRLDADVLAWFKAQ
GKGYQTRMNEILRDAMLKELKNHQ

Download         Length: 315 bp

>AT162232 NZ_CP071693:2693049-2693194 [Salmonella sp. SAL-045]
ATAAAAAGAGACCGAATACGATTCCTGTATTCGGTCCAGGGAAATGGCTCTTGGGAGAGAGCCGTGCGCTAAAAGTTGGC
ATTAATGCAGGCTAAATCGCCTTGCCCTTTAAGAATAGATGACGACGCCAGGTTTTCCAGTTTGCG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB N9P1T1

Antitoxin

Source ID Structure
AlphaFold DB N9PH66

References