Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/HicA(toxin)
Location 26600..27177 Replicon plasmid pBspH3
Accession NZ_CP055285
Organism Acinetobacter sp. NEB 394

Toxin (Protein)


Gene name hicA Uniprot ID N8ZPI0
Locus tag HUK62_RS18205 Protein ID WP_004812233.1
Coordinates 26929..27177 (-) Length 83 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID N8ZW11
Locus tag HUK62_RS18200 Protein ID WP_004812232.1
Coordinates 26600..26932 (-) Length 111 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HUK62_RS18175 22267..22626 - 360 WP_110975971.1 helix-turn-helix domain-containing protein -
HUK62_RS18180 22767..23699 + 933 WP_004812228.1 IS5-like element ISAha3 family transposase -
HUK62_RS18185 23783..25240 + 1458 WP_004695721.1 DASS family sodium-coupled anion symporter -
HUK62_RS18190 25521..26225 + 705 WP_001067783.1 IS6 family transposase -
HUK62_RS18195 26277..26471 + 195 Protein_26 arsenical-resistance protein -
HUK62_RS18200 26600..26932 - 333 WP_004812232.1 type II toxin-antitoxin system HicB family antitoxin Antitoxin
HUK62_RS18205 26929..27177 - 249 WP_004812233.1 type II toxin-antitoxin system HicA family toxin Toxin
HUK62_RS18210 27355..27783 + 429 WP_004786455.1 organic hydroperoxide resistance protein -
HUK62_RS18215 27790..28221 + 432 WP_000042163.1 MarR family transcriptional regulator -
HUK62_RS18220 28288..28701 - 414 Protein_31 transposase -
HUK62_RS18225 29026..31104 + 2079 WP_110975974.1 polyphosphate kinase 1 -
HUK62_RS18230 31222..31449 + 228 WP_004646177.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - - 1..85229 85229
- inside IScluster/Tn - - 2135..28716 26581


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(20-78)

Antitoxin

Uncharacterised protein family HicB

(54-101)


Sequences

Toxin        


Download         Length: 83 a.a.        Molecular weight: 9436.02 Da        Isoelectric Point: 10.1337

>T162231 WP_004812233.1 NZ_CP055285:c27177-26929 [Acinetobacter sp. NEB 394]
MGKTEKLLEKFGNSKNTFPYKDLVVLLSQLGYEKFEMAGSRVRFFNEKTEHMILLHRPHPENEIKGGALKAVKQALKQEG
FL

Download         Length: 249 bp

>T162231 NZ_CP071693:c2443686-2443531 [Salmonella sp. SAL-045]
ATGAAGCAGCAAAAGGCGATGTTAATCGCCCTGATCGTCATCTGTTTAACCGTCATAGTGACGGCACTGGTAACGAGGAA
AGACCTCTGCGAGGTACGAATCCGAACCGGCCAGACGGAGGTCGCTGTCTTCACAGCTTACGAACCTGAGGAGTAA

Antitoxin


Download         Length: 111 a.a.        Molecular weight: 12224.97 Da        Isoelectric Point: 4.8951

>AT162231 WP_004812232.1 NZ_CP055285:c26932-26600 [Acinetobacter sp. NEB 394]
MSYLKYKGYLGTIEPDLETGELFGKLAFIRDLITYEAETLKALEKAFQESVDGYLESCAELGKSPDQPFKGTFNVRISPE
LHRKAVLASSNSLNAFVSDAIQEKLMRLGA

Download         Length: 333 bp

>AT162231 NZ_CP071693:2443698-2443756 [Salmonella sp. SAL-045]
CCTTGCCTTTCGGCACGTAAGAGGCTAACCTACATGTGCAAAGCATGAAATTGGCCTCA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB N8ZPI0

Antitoxin

Source ID Structure
AlphaFold DB N8ZW11

References