Detailed information of TA system TA162229

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/upstrm_HI1419-dnstrm_HI1420
• Location: 101864..102452
• Replicon: plasmid pBspH2
• Accession: NZ_CP055284
• Organism: Acinetobacter sp. NEB 394

Toxin (Protein)

• Gene name: relE
• Uniprot ID: N9CKJ9
• Locus tag: HUK62_RS17785
• Protein ID: WP_004647749.1
• Coordinates: 102156..102452 (-)
• Length: 99 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: A0A3B7PNW4
• Locus tag: HUK62_RS17780
• Protein ID: WP_005093398.1
• Coordinates: 101864..102154 (-)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HUK62_RS17740	97047..97598	+	552	WP_119686772.1	hypothetical protein	-
HUK62_RS17745	98316..98630	-	315	WP_176538571.1	BrnA antitoxin family protein	-
HUK62_RS17750	98617..98904	-	288	WP_004884492.1	BrnT family toxin	-
HUK62_RS17755	99203..99601	+	399	WP_026438543.1	hypothetical protein	-
HUK62_RS17760	99684..99956	-	273	WP_176538572.1	helix-turn-helix domain-containing protein	-
HUK62_RS17765	99949..100269	-	321	Protein_116	type II toxin-antitoxin system RelE/ParE family toxin	-
HUK62_RS17770	100437..100868	+	432	WP_026438531.1	DUF600 family protein	-
HUK62_RS17775	101011..101757	+	747	WP_004645384.1	IS5-like element ISAba31 family transposase	-
HUK62_RS17780	101864..102154	-	291	WP_005093398.1	putative addiction module antidote protein	Antitoxin
HUK62_RS17785	102156..102452	-	297	WP_004647749.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
HUK62_RS17790	102562..102789	-	228	WP_176538573.1	hypothetical protein	-
HUK62_RS17795	102968..104187	+	1220	WP_176538574.1	IS3 family transposase	-
HUK62_RS17800	104239..104592	+	354	WP_176538592.1	SMI1/KNR4 family protein	-
HUK62_RS17805	104720..106045	+	1326	Protein_124	ISNCY family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	katA	1..161809	161809
	-	inside	IScluster/Tn	-	-	101011..122997	21986

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Addiction module killer protein, predicted (3-95)

Antitoxin

• Uncharacterized protein HI1420 (7-93)

Sequences

Toxin

Protein

• Length: 99 a.a.
• Molecular weight: 11584.25 Da
• Isoelectric Point: 5.8801

>T162229 WP_004647749.1 NZ_CP055284:c102452-102156 [Acinetobacter sp. NEB 394]
MIEIKRLPEFDEWLDGIKDNMTRIRLNRRLDKVQRGNWGDIKPLQDGVWEMREFFGSGWRMYYIQHGDVVIVMLGGGDKS
TQQQDIDRAVKLSKTLED

Nucleotide

• Length: 297 bp

>T162229 NZ_CP071693:836168-836566 [Salmonella sp. SAL-045]
ATGCTGAAATTCATGCTTGATACCAATACCTGTATTTTCACCATCAAAAATAAGCCCGAACACATCAGAGAACGCTTCAA
CCTCAATACATCCCGAATGTGTATCAGCTCCATCACCTTAATGGAGCTGATTTACGGTGCTGAAAAAAGCCTGGCGCCGG
AGCGTAATCTTGCCGTCGTGGAGGGATTTATCTCCCGCCTTGAGGTTCTGGATTACGATACACAGGCAGCGATACATACC
GGTCAAATCCGTGCCGAACTGGCCCGCAAGGGAACACCTGTCGGGCCTTATGACCAGATGATTGCTGGCCATGCCGGTAG
CCGCGGACTGGTCGTCGTCACAAACAATCTCCGCGAATTTGAACGCATTCCGGGTATCCGAATCGAAGACTGGTGCTAA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10533.20 Da
• Isoelectric Point: 5.6158

>AT162229 WP_005093398.1 NZ_CP055284:c102154-101864 [Acinetobacter sp. NEB 394]
MVKVADLPSFDMAESLKTEEDIVMYLNMVLEENDPAELAHALGVIAKARGMTQIAKEAGIGREALYKALRHDSAPRFDTI
NRVVNALGLKLTVQHA

Nucleotide

• Length: 291 bp

>AT162229 NZ_CP071693:835941-836168 [Salmonella sp. SAL-045]
ATGCACACAACACTTTTTTTTAGTAACCGGACCCAGGCAGTCAGATTGCCTAAATCGATATCCTTTCCGGAGGATGTGAA
ACATGTCGAAATCATTGCGGTTGGTAGAAGCAGAATCATTACTCCAGTCGGAGAATCCTGGGACAGCTGGTTCGATGGCG
AAGGCGCCAGTACTGATTTTATGAGTACCAGAGAACAACCCGCAGTACAGGAACGGGAAGGATTTTGA

Structures

Antitoxin

• AlphaFold DB: A0A3B7PNW4