Detailed information of TA system TA162227

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/HicA(toxin)
• Location: 94570..95150
• Replicon: plasmid pBspH2
• Accession: NZ_CP055284
• Organism: Acinetobacter sp. NEB 394

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: A0A7H8SLW2
• Locus tag: HUK62_RS17725
• Protein ID: WP_044425274.1
• Coordinates: 94570..94818 (+)
• Length: 83 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: A0A7H8SKS1
• Locus tag: HUK62_RS17730
• Protein ID: WP_035270373.1
• Coordinates: 94815..95150 (+)
• Length: 112 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HUK62_RS17685	90020..90361	+	342	WP_176538569.1	sulfatase-like hydrolase/transferase	-
HUK62_RS17690	90477..90983	+	507	Protein_101	IS6 family transposase	-
HUK62_RS17695	90979..91680	+	702	Protein_102	multicopper oxidase domain-containing protein	-
HUK62_RS17700	91667..92005	+	339	WP_004897649.1	hypothetical protein	-
HUK62_RS17705	91992..92936	+	945	WP_176538570.1	copper resistance protein B	-
HUK62_RS17710	93252..93563	+	312	WP_063454728.1	BrnT family toxin	-
HUK62_RS17715	93526..93840	+	315	WP_038350244.1	BrnA antitoxin family protein	-
HUK62_RS17720	93937..94464	+	528	WP_000537572.1	plasmid pRiA4b ORF-3 family protein	-
HUK62_RS17725	94570..94818	+	249	WP_044425274.1	type II toxin-antitoxin system HicA family toxin	Toxin
HUK62_RS17730	94815..95150	+	336	WP_035270373.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
HUK62_RS17735	95289..96743	-	1455	WP_004762546.1	SulP family inorganic anion transporter	-
HUK62_RS17740	97047..97598	+	552	WP_119686772.1	hypothetical protein	-
HUK62_RS17745	98316..98630	-	315	WP_176538571.1	BrnA antitoxin family protein	-
HUK62_RS17750	98617..98904	-	288	WP_004884492.1	BrnT family toxin	-
HUK62_RS17755	99203..99601	+	399	WP_026438543.1	hypothetical protein	-
HUK62_RS17760	99684..99956	-	273	WP_176538572.1	helix-turn-helix domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	katA	1..161809	161809

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (20-78)

Antitoxin

• Uncharacterised protein family HicB (54-102)

Sequences

Toxin

Protein

• Length: 83 a.a.
• Molecular weight: 9451.04 Da
• Isoelectric Point: 10.3195

>T162227 WP_044425274.1 NZ_CP055284:94570-94818 [Acinetobacter sp. NEB 394]
MGKTEKLLEKFGNSKNTFPYKDLVVLLNQLGYEKFEMAGSRVRFFNTKTEHMILLHRPHPENEIKGGALKSVKQALKQEG
FL

Nucleotide

• Length: 249 bp

>T162227 NZ_CP071693:358934-359209 [Salmonella sp. SAL-045]
ATGGGGCAAAGGGAAATTGAATATTCGGGACAGTTCCAGAAAGATGTAAAACGGGCACAAAAGCGTCATAAGGATGTCGG
CAAACTTAAAACACTTATGACGCTTCTGATTCACCACCCTTTTCCCCTTCCTGCCATTTATAAAGATCACCCGTTACAAG
GTTCATATAGCGGATACCGGGATGCCCATATTGAGCCTGACTGGATTCTTATTTATAAGATAACGGATGAATGCCTGCGA
TTTGAACGAACAGGAACGCATGCCGATTTATTTTAA

Antitoxin

Protein

• Length: 112 a.a.
• Molecular weight: 12411.14 Da
• Isoelectric Point: 4.7146

>AT162227 WP_035270373.1 NZ_CP055284:94815-95150 [Acinetobacter sp. NEB 394]
MSYLKYKGYLGTIEPDLETGELFGKLAFIRDLITYEAETLKTLEQAFQESVDGYLESCAELGKKPDQPFKGTFNVRISPE
LHRKAVLASSDNSLNAFVSDAIQEKLMRLGA

Nucleotide

• Length: 336 bp

>AT162227 NZ_CP071693:358671-358931 [Salmonella sp. SAL-045]
ATGGCTGCAAATGCGCTTGTTCGTGCCCGTATCGACGAAACGCTGAAGGACCAGGCGGCAGATGTCCTGGCTGAAATGGG
GTTAACCATTTCTGATTTGATACGGATTACACTGACTAAAGTTGCGCGTGAAAAGGCATTGCCTTTTGATTTGCGCATAC
CCAATGAGTTAACGTCACGAACGATAGAAAATAGCGAAGCTGGCGTTGATATTCATAAAGCCAAAGATGCTGATGATTTG
TTTGACCAGTTAGGGATCTGA

Structures

Toxin

• AlphaFold DB: A0A7H8SLW2

Antitoxin

• AlphaFold DB: A0A7H8SKS1