Detailed information of TA system TA162225

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/ParE-RelB
• Location: 10893..11421
• Replicon: plasmid pBspH2
• Accession: NZ_CP055284
• Organism: Acinetobacter sp. NEB 394

Toxin (Protein)

• Gene name: relE
• Uniprot ID: -
• Locus tag: HUK62_RS17245
• Protein ID: WP_119686978.1
• Coordinates: 11128..11421 (+)
• Length: 98 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: N9B5Q7
• Locus tag: HUK62_RS17240
• Protein ID: WP_000246756.1
• Coordinates: 10893..11138 (+)
• Length: 82 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HUK62_RS17225	6752..8272	+	1521	WP_049590605.1	catalase	-
HUK62_RS17230	8551..9695	+	1145	WP_176538547.1	IS3 family transposase	-
HUK62_RS17235	9715..10686	+	972	WP_176538590.1	sensor domain-containing diguanylate cyclase	-
HUK62_RS17240	10893..11138	+	246	WP_000246756.1	type II toxin-antitoxin system RelB/DinJ family antitoxin	Antitoxin
HUK62_RS17245	11128..11421	+	294	WP_119686978.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
HUK62_RS17250	11473..12444	-	972	WP_176538548.1	helix-turn-helix domain-containing protein	-
HUK62_RS17255	12577..13459	+	883	Protein_14	MBL fold metallo-hydrolase	-
HUK62_RS17260	13543..14085	+	543	WP_176538549.1	cysteine hydrolase	-
HUK62_RS17265	14172..14453	-	282	WP_000985610.1	putative addiction module antidote protein	-
HUK62_RS17270	14454..14660	-	207	Protein_17	type II toxin-antitoxin system RelE/ParE family toxin	-
HUK62_RS17275	14896..15915	+	1020	WP_004637109.1	cation transporter	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	katA	1..161809	161809
	-	inside	IScluster/Tn	-	-	16001..44383	28382

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (8-84)

Antitoxin

• RelB antitoxin/Antitoxin DinJ (1-71)

Sequences

Toxin

Protein

• Length: 98 a.a.
• Molecular weight: 11319.59 Da
• Isoelectric Point: 10.5382

>T162225 WP_119686978.1 NZ_CP055284:11128-11421 [Acinetobacter sp. NEB 394]
MTYKLLRHKDFKAEWEKLPVAIRDQFKKKLAKVIEQPHIPKNMLRGDLAGCYKIKLLKAGVRLVYQVKDDQVVILLITVG
KRADSIVYDEAKKRIKD

Nucleotide

• Length: 294 bp

>T162225 NZ_CP071693:200443-200928 [Salmonella sp. SAL-045]
GTGGGACGTGTAACAGCACCAGAACCTTTGTCCGCTTTTCATCAGGTAGCTGAGTTCGTCAGCGGTGAAGCTGTGCTCGA
TGACTGGTTGAAGCAAAAGGGCCTCAAAAACCAGGCTCTCGGAGCGGCCAGAACATTTGTGGTGTGCAAGAAAGACACGA
AGCAAGTAGCCGGTTTTTACTCTCTGGCCACCGGTAGCGTCAACCATACAGAAGTGACAGGCAACCTTCGGCGTAACATG
CCAGATCCCATCCCTGTCATTATACTTGCCCGTCTTGCTGTCGATCTCTCATTCCATGGAAAAGGGCTTGGTGCTGATTT
ACTTCATGATGCAGTGCTTCGTTGCTATCGGGTTGCCGAGAATATTGGTGTACGTGCAATCATGGTTCATGCACTTACCG
AAGAAGCCAAAAATTTCTACATTCACCATGGTTTCAAATCATCACAAACTCAGCAGCGAACATTGTTCCTTAGGCTCCCT
CAATAG

Antitoxin

Protein

• Length: 82 a.a.
• Molecular weight: 9235.61 Da
• Isoelectric Point: 4.3142

>AT162225 WP_000246756.1 NZ_CP055284:10893-11138 [Acinetobacter sp. NEB 394]
MATVNFRVDEALKEKSYSILKEQGIAPTDFFTSILEYVATTGKLPVKKALLSEEDEELLALVRKRINDPKEMFEEVTLDD
L

Nucleotide

• Length: 246 bp

>AT162225 NZ_CP071693:200168-200455 [Salmonella sp. SAL-045]
ATGCTATACAAGGGGTGTCTCATGAAATCAGATGTTCAACTTAACCTTAGAGCTAAGGAATCGCAGCGGGCGCTCATTGA
TGCAGCTGCGGAAATCCTTCACAAGTCACGTACAGATTTCATTCTGGAAACGGCCTGCCAGGCTGCCGAGAAAGTGATCC
TTGACCGCCGTGTATTTAACTTTAACGATGAGCAATATGAGGAGTTCATCAATCTGCTTGATGCACCAGTCGCAGATGAT
CCCGTTATCGAAAAACTGCTGGCAAGGAAACCTCAGTGGGACGTGTAA

Structures

Antitoxin

• AlphaFold DB: N9B5Q7