Detailed information of TA system TA162224

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): cptAB/Cpta(toxin)
• Location: 3248832..3249469
• Replicon: chromosome
• Accession: NZ_CP055277
• Organism: Acinetobacter sp. NEB 394

Toxin (Protein)

• Gene name: cptA
• Uniprot ID: D0SF64
• Locus tag: HUK62_RS15760
• Protein ID: WP_005401274.1
• Coordinates: 3248832..3249218 (-)
• Length: 129 a.a.

Antitoxin (Protein)

• Gene name: cptB
• Uniprot ID: -
• Locus tag: HUK62_RS15765
• Protein ID: WP_171065375.1
• Coordinates: 3249224..3249469 (-)
• Length: 82 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HUK62_RS15740	3244165..3246447	-	2283	WP_176538323.1	TRAP transporter large permease subunit	-
HUK62_RS15745	3246630..3247202	-	573	WP_176538324.1	rhombosortase	-
HUK62_RS15750	3247274..3247837	-	564	WP_171293170.1	hypothetical protein	-
HUK62_RS15755	3247834..3248076	-	243	WP_176538325.1	hypothetical protein	-
HUK62_RS15760	3248832..3249218	-	387	WP_005401274.1	hypothetical protein	Toxin
HUK62_RS15765	3249224..3249469	-	246	WP_171065375.1	succinate dehydrogenase assembly factor 2	Antitoxin
HUK62_RS15770	3249674..3250846	+	1173	WP_176538326.1	acyl-CoA dehydrogenase family protein	-
HUK62_RS15775	3250882..3251673	-	792	Protein_3046	alpha/beta hydrolase	-
HUK62_RS15780	3251805..3252443	-	639	Protein_3047	NAD(P)/FAD-dependent oxidoreductase	-
HUK62_RS15785	3252462..3253688	-	1227	Protein_3048	transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Inner membrane protein YgfX (5-127)

Antitoxin

• Flavinator of succinate dehydrogenase (6-78)

Sequences

Toxin

Protein

• Length: 129 a.a.
• Molecular weight: 15302.28 Da
• Isoelectric Point: 10.4419

>T162224 WP_005401274.1 NZ_CP055277:c3249218-3248832 [Acinetobacter sp. NEB 394]
MEHRHFQLKPSRLAYVFQLSIYAVLMLVLYQLLPLGLWLIALVAGFVLYLFFLRGPQHFQLQHLDGRDWSLSLQKQSNVQ
RVQISHIVDHRAYIVIYFQHFRAKPMLVWCDQLPITQWKALKILTKMQ

Nucleotide

• Length: 387 bp

>T162224 NZ_CP071692:c23179-22898 [Salmonella sp. SAL-020]
ATGACCTATGAACTGGCTTTTGACCGCAGAGCACTGAAGGAATGGCAGAAACTCGGCCACACCATCCGTGAACAATTCAA
AAAGAAGCTGGCAGAACGGCTGGAAAATCCACGCGTACCCGCAGCCCGGTTACATGGTCATGCTGATCGCTATAAAATCA
AACTTCGTGCATCTGGCTACAGACTTGTATATCAAGTCATTGATGAGAAAGTCGTTTTACTCGTTATTGCTGTTGGAAGA
AGGGAAAGCAGCGAAGTTTATCAGATTGCAGATATGCGCTGA

Antitoxin

Protein

• Length: 82 a.a.
• Molecular weight: 9691.18 Da
• Isoelectric Point: 5.1473

>AT162224 WP_171065375.1 NZ_CP055277:c3249469-3249224 [Acinetobacter sp. NEB 394]
MSLEERKVIYRARRGLKEIDVYFDPYVKNYYLQADPAEKAMFAALVEQEDPDLLDWFMEVSEPPRPEMKTLIQKLKHYVH
G

Nucleotide

• Length: 246 bp

>AT162224 NZ_CP071692:c23420-23169 [Salmonella sp. SAL-020]
ATGAGCTATCAAATTCTGACAACCACAGCGGCCAGTATTACTGACCTGAAAAAAAATCCTATGGGAACCGTAGCTGAAGG
TGAAGGGGACGCTGTTGCGATCCTGAACCGAAACGAACCGGCGTTCTATTGCGTTCCACCAAAACTTTACGCCTACTATC
GGGAACTCGCTGAAGATGCTGAGTTAAACGCTGTTGCTGATGAGCGCATGAAAAACCCGGAAATTGTGAAGGTTAACCTG
GATGACCTATGA

Structures

Toxin

• AlphaFold DB: A0A7H8SJA2