Detailed information of TA system TA162223

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/-
• Location: 2981242..2981819
• Replicon: chromosome
• Accession: NZ_CP055277
• Organism: Acinetobacter sp. NEB 394

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: N8ZPI0
• Locus tag: HUK62_RS14395
• Protein ID: WP_004812233.1
• Coordinates: 2981242..2981490 (+)
• Length: 83 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: N8ZW11
• Locus tag: HUK62_RS14400
• Protein ID: WP_004812232.1
• Coordinates: 2981487..2981819 (+)
• Length: 111 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HUK62_RS14360	2976621..2977430	+	810	WP_176538199.1	cytochrome c biogenesis protein CcsA	-
HUK62_RS14365	2977631..2978272	-	642	WP_078426704.1	hypothetical protein	-
HUK62_RS14370	2978352..2978660	+	309	Protein_2784	type VI secretion system tip protein VgrG	-
HUK62_RS14375	2978657..2979382	+	726	WP_176538200.1	hypothetical protein	-
HUK62_RS14380	2979390..2980322	-	933	WP_176538201.1	IS5 family transposase	-
HUK62_RS14385	2980378..2980608	+	231	Protein_2787	hypothetical protein	-
HUK62_RS14390	2980623..2981135	+	513	WP_078426715.1	hypothetical protein	-
HUK62_RS14395	2981242..2981490	+	249	WP_004812233.1	type II toxin-antitoxin system HicA family toxin	Toxin
HUK62_RS14400	2981487..2981819	+	333	WP_004812232.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
HUK62_RS14405	2981941..2982321	+	381	WP_176538202.1	hypothetical protein	-
HUK62_RS14410	2982396..2982794	-	399	WP_120402731.1	hypothetical protein	-
HUK62_RS14415	2982965..2983900	+	936	WP_176538203.1	tRNA-dihydrouridine synthase	-
HUK62_RS14420	2983897..2984952	-	1056	WP_176538204.1	DUF1176 domain-containing protein	-
HUK62_RS14425	2984991..2985395	-	405	WP_176538205.1	NUDIX domain-containing protein	-
HUK62_RS14430	2985602..2986048	-	447	WP_176538206.1	nuclear transport factor 2 family protein	-
HUK62_RS14435	2986159..2986494	-	336	WP_004695228.1	YegP family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	flank	IS/Tn	-	-	2979390..2980259	869

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (20-78)

Antitoxin

• Uncharacterised protein family HicB (54-101)

Sequences

Toxin

Protein

• Length: 83 a.a.
• Molecular weight: 9436.02 Da
• Isoelectric Point: 10.1337

>T162223 WP_004812233.1 NZ_CP055277:2981242-2981490 [Acinetobacter sp. NEB 394]
MGKTEKLLEKFGNSKNTFPYKDLVVLLSQLGYEKFEMAGSRVRFFNEKTEHMILLHRPHPENEIKGGALKAVKQALKQEG
FL

Nucleotide

• Length: 249 bp

>T162223 NZ_CP071691:c61626-61321 [Salmonella sp. SAL-020]
ATGCAGTTTAAGGTTTACACCTGTAAAAGGGAGAGTCGCTACCGGCTGTTTGTGGACGTGCAGAGCGACATCATTGACAC
CCCCGGACGGCGGATGGCTGTCCCGCTGGTCAGCGCCCGTCTGCTGTCGGAAAAGGTTCCCCGCGATCTTTACCCGGTGA
TGCATATCGGGGATGAGCCTTACCGCCTGCTGACCACGGATATGACCAGTGTGCCGGCCACCGTCATCGGGGAAGAGGTG
GCCGATCTCAGCCTCCGGGAGAACGATATCAAAAACGCCATTAACCTGATGTTCCGGGGGATCTGA

Antitoxin

Protein

• Length: 111 a.a.
• Molecular weight: 12224.97 Da
• Isoelectric Point: 4.8951

>AT162223 WP_004812232.1 NZ_CP055277:2981487-2981819 [Acinetobacter sp. NEB 394]
MSYLKYKGYLGTIEPDLETGELFGKLAFIRDLITYEAETLKALEKAFQESVDGYLESCAELGKSPDQPFKGTFNVRISPE
LHRKAVLASSNSLNAFVSDAIQEKLMRLGA

Nucleotide

• Length: 333 bp

>AT162223 NZ_CP071691:c61846-61628 [Salmonella sp. SAL-020]
ATGAAGCAGCGAATTACAGTGACAGTGGACAGCGACAGCTATCAGCTGCTCAAGGCATACGACGTGAATATCTCCGGTCT
GGTCAGTACGACCATGCAGAACGAAGCCCGCCGTCTGCGAGCCGAACGCTGGCAGGAAGAAAATCGGGAAGGTATGGCTG
AGGTGGCCAGCTTTATAGAGGCTAACGGATCGTTTGCTGACGACAACAGGAACTGGTGA

Structures

Toxin

• AlphaFold DB: N8ZPI0

Antitoxin

• AlphaFold DB: N8ZW11