Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type V Classification (family/domain) ghoTS/ghoT-GhoS
Location 4329396..4329893 Replicon chromosome
Accession NZ_CP055259
Organism Escherichia coli strain AH62

Toxin (Protein)


Gene name ghoT Uniprot ID L4JJG6
Locus tag HU676_RS20860 Protein ID WP_001173343.1
Coordinates 4329396..4329569 (-) Length 58 a.a.

Antitoxin (Protein)


Gene name ghoS Uniprot ID L4JK92
Locus tag HU676_RS20865 Protein ID WP_000398619.1
Coordinates 4329597..4329893 (-) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HU676_RS20850 4326066..4327523 + 1458 WP_176256623.1 dipeptide/tripeptide permease DtpC -
HU676_RS20855 4327760..4329277 + 1518 WP_001295074.1 lysine--tRNA ligase -
HU676_RS20860 4329396..4329569 - 174 WP_001173343.1 type V toxin-antitoxin system toxin GhoT Toxin
HU676_RS20865 4329597..4329893 - 297 WP_000398619.1 type V toxin-antitoxin system endoribonuclease antitoxin GhoS Antitoxin
HU676_RS20870 4330120..4330392 - 273 WP_000405647.1 N-acetyltransferase -
HU676_RS20875 4330404..4330634 - 231 WP_000371704.1 (4Fe-4S)-binding protein -
HU676_RS20880 4330815..4332446 + 1632 WP_001565376.1 sensor histidine kinase -
HU676_RS20885 4332443..4333162 + 720 WP_000611288.1 two-component system response regulator DcuR -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin GhoT/OrtT

(4-57)

Antitoxin

Endoribonuclease antitoxin GhoS

(5-94)


Sequences

Toxin        


Download         Length: 58 a.a.        Molecular weight: 6555.06 Da        Isoelectric Point: 10.2288

>T162184 WP_001173343.1 NZ_CP055259:c4329569-4329396 [Escherichia coli]
MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITWPMSLPVALLFSLF

Download         Length: 174 bp

>T162184 NZ_CP071681:c505637-505344 [Rhizobium ruizarguesonis]
ATGAGTTTTAAGCTTTCTGCCGAAGCGGAAGATGACATTATTGCGATCGCCGAGCAAGGTATCCGTATGTTCGGGCCGGG
CCAGGCAAAGCGATACCACGATGAACTTTTCGCATTATTTGATCTGATAGCTGTCAATCCACGCATCGCGCGCGAACGGG
ATGAGATTGATCCGCCCGTTAGAATTCATCCCTTCAAAGCGCATCTAATCGTTTATCGGATCGAAGATGATGAGACAATT
TTTGTAATACGAACTCGCCACGCGCATGAAGATTGGGCAAGTGACTCAATTTAG

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11467.97 Da        Isoelectric Point: 4.1705

>AT162184 WP_000398619.1 NZ_CP055259:c4329893-4329597 [Escherichia coli]
MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEYGIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDI
DFDLNIMTVDDYFRQFYK

Download         Length: 297 bp

>AT162184 NZ_CP071681:c505876-505634 [Rhizobium ruizarguesonis]
ATGGCCACGATGAACGTATCTCTTCCAGGCCCGATGAAGGATTGGGTTGAGGCCCAGGCCAGGACCGGGCGATATTCGAA
TGCTAGCGACTACGTGCGCGACCTGATCCGACGCGACCAGACCCGAAATGACAAGATCGCCGCCATGCAAAGCTTCGTCG
AGGCCGGGCTCCAAAGTGGTGTCGGCATTCGATCAAAGGATGAACTCTTTGCTGAGGCAATGGCTCGTGCCAACAAGTCA
TGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9QI41

Antitoxin

Source ID Structure
PDB 2LLZ
AlphaFold DB A0A7U9QBH6

References