Detailed information of TA system TA162177

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 2823126..2823970
• Replicon: chromosome
• Accession: NZ_CP055259
• Organism: Escherichia coli strain AH62

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: B1LJY4
• Locus tag: HU676_RS13695
• Protein ID: WP_000854686.1
• Coordinates: 2823126..2823509 (-)
• Length: 128 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: A0A8E0J1S3
• Locus tag: HU676_RS13700
• Protein ID: WP_001285602.1
• Coordinates: 2823590..2823970 (-)
• Length: 127 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HU676_RS13655	2818128..2818619	-	492	WP_001300785.1	DUF1097 domain-containing protein	-
HU676_RS13660	2818721..2819275	-	555	WP_001001902.1	molecular chaperone YcdY	-
HU676_RS13665	2819299..2820036	-	738	WP_000283664.1	zinc-binding phosphatase	-
HU676_RS13670	2820091..2821029	-	939	WP_000351315.1	glyoxylate/hydroxypyruvate reductase GhrA	-
HU676_RS13680	2821500..2822342	-	843	WP_001274547.1	DUF4942 domain-containing protein	-
HU676_RS13685	2822427..2822624	-	198	WP_000839253.1	DUF957 domain-containing protein	-
HU676_RS13690	2822641..2823129	-	489	WP_001054233.1	hypothetical protein	-
HU676_RS13695	2823126..2823509	-	384	WP_000854686.1	TA system toxin CbtA family protein	Toxin
HU676_RS13700	2823590..2823970	-	381	WP_001285602.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
HU676_RS13705	2823981..2824664	-	684	WP_000086769.1	hypothetical protein	-
HU676_RS13710	2824683..2824904	-	222	WP_000692298.1	DUF987 domain-containing protein	-
HU676_RS13715	2824967..2825443	-	477	WP_001186726.1	RadC family protein	-
HU676_RS13720	2825459..2825944	-	486	WP_000214307.1	antirestriction protein	-
HU676_RS13725	2826036..2826854	-	819	WP_001234732.1	DUF945 domain-containing protein	-
HU676_RS13730	2826954..2827187	-	234	WP_001119719.1	DUF905 family protein	-
HU676_RS13735	2827266..2827720	-	455	Protein_2689	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	csgB / csgD / csgE / csgF / csgG	2814509..2859650	45141

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-113)

Antitoxin

• Antitoxin CbeA (18-117)

Sequences

Toxin

Protein

• Length: 128 a.a.
• Molecular weight: 14272.28 Da
• Isoelectric Point: 6.8614

>T162177 WP_000854686.1 NZ_CP055259:c2823509-2823126 [Escherichia coli]
MKTLPDTHVRAASRCPSPVEIWQTLLTRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYVLVRTDQPGF
SAGAPSSQLINSIDILRARRATGLMTRDNYRMVNNITLGKHPEEAKQ

Nucleotide

• Length: 384 bp

>T162177 NZ_CP071635:c186064-185645 [Rhizobium sp. NZLR1]
ATGGCGCGCTACATGCTCGATACGAACATGTGTATCTACCTGATGAAAAACCAGCCGGAGCAGGTCGCTCGCCGCTTTGC
CAGTTGCTATGTGGGGGACGTGGTGATGTCCGCCATCACCTTCGCGGAACTCGAGTACGGTGTTTCCGCATCCGATGATC
CGGAACGAGAACTTGATAATCTCTCCGCCCTGGCCGAGCTAATCACGGTAGAGCCGTTTGGGATCGCAGCAGCGCGTGCC
TACGGTCCGGTTCGAATGGCGACGAGAGACCGGAAGAAAGATCATCTCGACAAACTGATCGCATCGCATGCGATCGCTCT
GGACACGGTATTGGTGACCAACAACACGCGGGACTTCCTGGCGTATCCCGGACTGAAGCTCGAAAACTGGCTGGACGCGG
AACGCAGAGAAACCAATTAA

Antitoxin

Protein

• Length: 127 a.a.
• Molecular weight: 13938.69 Da
• Isoelectric Point: 5.0823

>AT162177 WP_001285602.1 NZ_CP055259:c2823970-2823590 [Escherichia coli]
VSDTLPGTTLPDDNKDRPWWGLPCTVTPCFGARLVQEGNRLHYLADRAGIRGRFSNADAYHLDQAFPLLMKQLELMLTSG
ELNPRHQHTVTLYAKGLTCDADTLGSCGYVYLAVYPATETESNPPE

Nucleotide

• Length: 381 bp

>AT162177 NZ_CP071635:c186300-186064 [Rhizobium sp. NZLR1]
ATGCACACCACTAAAGCTTTCAAGAATGGAAATTCCCAAGCGGTTCGCATACCGGCGGAACTGGCGTTTGAGCGAACCGA
TGTTGAGTTGGAGATCGAGCGCATCGGCGACGAGCTTCGCATCCGTCCTGTGCATCGCTCCCTGGCCGGCGTTCTGGATG
TATTCGCCCGCTTCTCTCCGGATTTCATGCGTGACGGAAGAGAGTCCCATGAGCAAGACGAGCGGGACAAGATCTGA