Detailed information of TA system TA162176

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hipBA/HipA-HipB
• Location: 2324357..2325945
• Replicon: chromosome
• Accession: NZ_CP055259
• Organism: Escherichia coli strain AH62

Toxin (Protein)

• Gene name: hipA
• Uniprot ID: -
• Locus tag: HU676_RS11175
• Protein ID: WP_176256532.1
• Coordinates: 2324623..2325945 (+)
• Length: 441 a.a.

Antitoxin (Protein)

• Gene name: hipB
• Uniprot ID: -
• Locus tag: HU676_RS11170
• Protein ID: WP_001301023.1
• Coordinates: 2324357..2324623 (+)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HU676_RS11160	2319438..2322623	+	3186	WP_176256652.1	transporter	-
HU676_RS11165	2322662..2323474	+	813	Protein_2182	autotransporter outer membrane beta-barrel domain-containing protein	-
HU676_RS11170	2324357..2324623	+	267	WP_001301023.1	type II toxin-antitoxin system antitoxin HipB	Antitoxin
HU676_RS11175	2324623..2325945	+	1323	WP_176256532.1	type II toxin-antitoxin system serine/threonine protein kinase toxin HipA	Toxin
HU676_RS11180	2326141..2327595	+	1455	WP_001549221.1	site-specific integrase	-
HU676_RS11185	2327701..2329239	-	1539	WP_176256509.1	IS66 family transposase	-
HU676_RS11190	2329289..2329636	-	348	WP_000612591.1	IS66 family insertion sequence element accessory protein TnpB	-
HU676_RS11195	2329633..2330013	-	381	WP_001171554.1	IS66 family insertion sequence hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	2318675..2363139	44464

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HipA, N-terminal subdomain 1 (4-103)

Antitoxin

• Cro/C1-type helix-turn-helix domain (14-69)

Sequences

Toxin

Protein

• Length: 441 a.a.
• Molecular weight: 49305.84 Da
• Isoelectric Point: 8.2482

>T162176 WP_176256532.1 NZ_CP055259:2324623-2325945 [Escherichia coli]
MPKLVTWMNNQRVGELTKLANGAHTFKYAPEWLASRYARPLSLSLPLQRGNITSDAVFNFFDNLLPDSPIVRDRIVKRYH
AKSRQPFDLLSEIGRDSVGAVTLIPEDETVTHPIMAWEKLTEARLEEVLTAYKADIPLGMIREENDFRISVAGAQEKTAL
LRIGNDWCIPKGITPTTHIIKLPIGEIRQPNATLDLSQSVDNEYYCLLLAKELGLNVPDAEIIKAGNVRALAVERFDRRW
NAERTVLLRLPQEDMCQTFGLPSSVKYESDGGPGIARIMAFLMGSSEALKDRYDFMKFQVFQWLIGATDGHTKNFSVFIQ
AGGSYRLTPFYDIISAFPVLGGTGIHISDLKLAMGLNASKGKKTAIDKIYPRHFLATAKVLRFPEVQMHEILSDFARMIP
AALDNVKTSLPTDFPENVVTAVESNVLRLHGRLSREYGSK

Nucleotide

• Length: 1323 bp

>T162176 NZ_CP071634:398226-398549 [Rhizobium sp. NZLR1]
GTGAGGCGCGGTGACATCGTAACCGTTGCCATCACCGGCGATTTCGGCAAACCGCGTCCGGCGCTCATCATCCAGGCGGA
TCAGTTTGACGCAACCGGCACGGTTACCGTGCTGCCGATTTCCGGCACCCTCATCGACGCGCCGCTCATTCGGCCGACAA
TCGAGCCGACAGCGCTCAGCGGTTTGCGCAAAGCCTCGCAGGTGAAGGTGGACAAAAGCATGTCGGTGAAACGGGAACGA
ATTGGGCCGATAATCGGTCGCCTGGAAGACGATGCTCTCTTGGCAGTCACAAGATCGCTCGCCGTGTTTTTGGGTATCGC
CTAA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 10016.38 Da
• Isoelectric Point: 6.2267

>AT162176 WP_001301023.1 NZ_CP055259:2324357-2324623 [Escherichia coli]
MMSFQKIYSPTQLANAMKLVRQQNGWTQSELAKKIGIKQATISNFENNPDNTTLTTFFKILQSLELSMTLCDAKNASPES
TEQQNLEW

Nucleotide

• Length: 267 bp

>AT162176 NZ_CP071634:398005-398229 [Rhizobium sp. NZLR1]
ATGGCAACGCCGGTCAACGAAAGAGTTCAAAAGCGCCGCGAGGCTTTGCGGGCCGCTGGACTGCGTCCCGTGCAAATATG
GGTTCCGGATACCCGTCGCCCTGGTTTTGCCGACGAATGCCATCGTCAGTCCGTTCAGACAGCTCTTGCCGATGCAGGCG
ATCGCGATTTGCAGGATTTCCTCGATGCCGCTCTCGCCGACATGGACCATGATGGTGAAGCGTGA