Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 856430..857084 Replicon chromosome
Accession NZ_CP055259
Organism Escherichia coli strain AH62

Toxin (Protein)


Gene name cptA Uniprot ID S1EEB2
Locus tag HU676_RS04145 Protein ID WP_000244777.1
Coordinates 856677..857084 (+) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag HU676_RS04140 Protein ID WP_000354046.1
Coordinates 856430..856696 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HU676_RS04115 851599..852342 + 744 WP_000951962.1 SDR family oxidoreductase -
HU676_RS04120 852399..853832 - 1434 WP_001338826.1 6-phospho-beta-glucosidase BglA -
HU676_RS04125 853877..854188 + 312 WP_001182954.1 N(4)-acetylcytidine aminohydrolase -
HU676_RS04130 854352..855011 + 660 WP_000250274.1 hemolysin III family protein -
HU676_RS04135 855207..856187 - 981 WP_000886062.1 tRNA-modifying protein YgfZ -
HU676_RS04140 856430..856696 + 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
HU676_RS04145 856677..857084 + 408 WP_000244777.1 toxin CptA Toxin
HU676_RS04150 857124..857645 - 522 WP_001055874.1 flavodoxin FldB -
HU676_RS04155 857757..858653 + 897 WP_000806638.1 site-specific tyrosine recombinase XerD -
HU676_RS04160 858678..859388 + 711 WP_000715214.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
HU676_RS04165 859394..861127 + 1734 WP_000813201.1 single-stranded-DNA-specific exonuclease RecJ -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16031.96 Da        Isoelectric Point: 11.5202

>T162166 WP_000244777.1 NZ_CP055259:856677-857084 [Escherichia coli]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWSIVKAPWMIKSGMMLRLRSDGGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T162166 NZ_CP071626:5185396-5185686 [Rhizobium leguminosarum]
ATGAAATACCGGACAACCATCGAAGCGGATCAGGACATAGTCGAGCTCTACATTCTCGGTGCCGATCAGTTTGGCGCTTT
ACAGTCGGAGCAATACGTCGATGACTTGTTCGACACATTTGAGTTGCTGGCCGATAATCCACGCATGGCCCGTGAGCGCC
GTGAGCTAAACCCGCCGACGCGACTTCATCCCTACCTTGCGCATATGATTGCCTACACGATACGCGAGGAGGATATTTTG
ATTGTTCGCGTGCTGCACGGGCGCGAGGATTGGCAAAGCCTCTTTGCGTAG

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT162166 WP_000354046.1 NZ_CP055259:856430-856696 [Escherichia coli]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT162166 NZ_CP071626:5185151-5185399 [Rhizobium leguminosarum]
ATGGCGACGATGAATGTTTCCCTGCCCGATCCAATGAAGGAATGGGTGGACGCTCAGACAAAAACCGGGCGTTACAGCAA
TGCGAGCGATTATGTCCGCGACCTGATACGGCGTGATCAAGAGCGCGCAGGTAAGCTGGCGGAATTGCAACGTTTGATCA
CGGACGGGCTGGAAAGCGGCGTCAGTGATCGTTCCAAGGCCGATATTCTTCGAGAAGCGCGCGAACGGCTGGCTGCGCGG
CGTGTATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9LFV7

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References