Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) phd-doc/Doc-Phd
Location 13806..14407 Replicon plasmid pAH25-2
Accession NZ_CP055258
Organism Escherichia coli strain AH25

Toxin (Protein)


Gene name doc Uniprot ID U9YA20
Locus tag HUU84_RS25155 Protein ID WP_001216045.1
Coordinates 13806..14186 (-) Length 127 a.a.

Antitoxin (Protein)


Gene name phd Uniprot ID U9YQH9
Locus tag HUU84_RS25160 Protein ID WP_001190712.1
Coordinates 14186..14407 (-) Length 74 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HUU84_RS25130 9246..10730 - 1485 WP_000124150.1 terminase -
HUU84_RS25135 10730..11923 - 1194 WP_000219607.1 hypothetical protein -
HUU84_RS25140 12010..12462 - 453 WP_016236875.1 hypothetical protein -
HUU84_RS25145 12551..13594 - 1044 WP_094319660.1 DUF968 domain-containing protein -
HUU84_RS25150 13622..13801 - 180 WP_000113018.1 hypothetical protein -
HUU84_RS25155 13806..14186 - 381 WP_001216045.1 type II toxin-antitoxin system death-on-curing family toxin Toxin
HUU84_RS25160 14186..14407 - 222 WP_001190712.1 type II toxin-antitoxin system Phd/YefM family antitoxin Antitoxin
HUU84_RS25165 14590..16146 + 1557 WP_101973138.1 type I restriction-modification system subunit M -
HUU84_RS25170 16143..17348 + 1206 WP_001293319.1 restriction endonuclease subunit S -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid aph(3')-Ia / aph(6)-Id / aph(3'')-Ib / dfrA12 / aadA2 / qacE / sul1 / aac(3)-IVa / aph(4)-Ia - 1..116030 116030


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Death on curing protein

(3-120)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-51)


Sequences

Toxin        


Download         Length: 127 a.a.        Molecular weight: 13588.29 Da        Isoelectric Point: 5.1514

>T162162 WP_001216045.1 NZ_CP055258:c14186-13806 [Escherichia coli]
MRHISPEELIALHDANISRYGGLPGMSDPGRAEAIIGRVQARVAYEEITDLFEVSATYLVATARGHIFNDANKRTALNSA
LLFLRRNGVQVFDSPELADLTVGAATGEISVSSVADTLRRLYGSAE

Download         Length: 381 bp

>T162162 NZ_CP071626:c3405173-3404760 [Rhizobium leguminosarum]
ATGACGGCCTGGATGCTCGATACCAATATGGTGAGCCATGTCATCAAGGGCGATCGGCCGGAGATCATCGACAGGTTGGC
CGCGCTTCCGATGGAGGACATCGTCATCTCATCGATCACGGAAGGGGAGCTGTTCTACGGTCTTGCCAAGCGAGGCTATC
CGAAGGTGCTGTCGGAACGGATCCGCCAATTCCTGCTCCGGGTCGACGTTCTCCCTTGGGATCACGATGTCACGCGAACT
TACGGCGACCTGCGCGCTGCCTGTGAAGCCAAAGGGGTGACTCTGGCGCCCCTCGATATGATGATTGCCGCTCATGCCGT
TGCAACTGCCGCAACCCTCGTCACGCGCGACAAGGCTTTTGCTCGCGTTTCGGAGCCTTTGAAGGTCGATGACTGGGCGA
ATGAAAGAAGCTGA

Antitoxin


Download         Length: 74 a.a.        Molecular weight: 8133.10 Da        Isoelectric Point: 4.7708

>AT162162 WP_001190712.1 NZ_CP055258:c14407-14186 [Escherichia coli]
MQSINFRTARGNLSEVLNNVEAGEEVEITRRGREPAVIVSKATFEAYKKAALDAEFASLFDTLDSTNKELVNR

Download         Length: 222 bp

>AT162162 NZ_CP071626:c3405433-3405170 [Rhizobium leguminosarum]
ATGGCAACCAGAACAGCCAAGATTTTTACGACCGGCCGCAGCCAAGCGGTCAGGCTCCCTGCGGAGTTCCGCTTCGAGGA
GAGCGAGGTGTTCGTCCGTCGTGATCCGAAGACCGGCGATGTCATTCTGTCACGCAAGCCGGATTCGTGGGATGGTCTCT
TTGAGCTTTATGGCAAGGATCGGGTTCCGGACGATTTCCTGGGGCCAGATGATCGCCACCAGCCTTCGCATGACCGTGAT
CCGTTCGAGGGTTGGAAGGAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829CLP7

Antitoxin

Source ID Structure
AlphaFold DB A0A829CJB6

References