Detailed information of TA system TA162161

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/-
• Location: 11793..12385
• Replicon: plasmid pAH25-1
• Accession: NZ_CP055257
• Organism: Escherichia coli strain AH25

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: A0A1I9ZJC5
• Locus tag: HUU84_RS24770
• Protein ID: WP_000513532.1
• Coordinates: 11793..12041 (+)
• Length: 83 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: A0A1I9ZJ49
• Locus tag: HUU84_RS24775
• Protein ID: WP_000681609.1
• Coordinates: 12038..12385 (+)
• Length: 116 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HUU84_RS24735	6862..7116	+	255	WP_001230709.1	hypothetical protein	-
HUU84_RS24740	7113..7355	+	243	WP_000151456.1	hypothetical protein	-
HUU84_RS24745	7492..7824	+	333	WP_001393253.1	WbuC family cupin fold metalloprotein	-
HUU84_RS24750	7871..8746	-	876	WP_015387340.1	class A extended-spectrum beta-lactamase CTX-M-55	-
HUU84_RS24755	8999..10261	-	1263	WP_000608644.1	IS1380-like element ISEc9 family transposase	-
HUU84_RS24760	10713..10895	+	183	WP_071599349.1	hypothetical protein	-
HUU84_RS24765	11011..11304	+	294	WP_000673428.1	hypothetical protein	-
HUU84_RS24770	11793..12041	+	249	WP_000513532.1	type II toxin-antitoxin system HicA family toxin	Toxin
HUU84_RS24775	12038..12385	+	348	WP_000681609.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
HUU84_RS24780	12473..12655	-	183	WP_001428470.1	hypothetical protein	-
HUU84_RS24785	13157..13456	+	300	WP_034169410.1	type II toxin-antitoxin system HicB family antitoxin	-
HUU84_RS24790	13716..14042	-	327	WP_000206697.1	hypothetical protein	-
HUU84_RS24795	14044..14484	-	441	WP_023223352.1	hypothetical protein	-
HUU84_RS24800	14504..15211	-	708	WP_000108794.1	hypothetical protein	-
HUU84_RS24805	15213..15965	-	753	WP_000797966.1	conjugal transfer protein TraL	-
HUU84_RS24810	15983..16384	-	402	WP_021575837.1	hypothetical protein	-
HUU84_RS24815	16752..17093	+	342	WP_000730722.1	plasmid mobilization relaxosome protein MobC	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	blaCTX-M-55 / mcr-1.1	-	1..64941	64941
	-	flank	IS/Tn	blaCTX-M-55	-	7871..10261	2390

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (20-81)

Antitoxin

• Uncharacterised protein family HicB (54-104)

Sequences

Toxin

Protein

• Length: 83 a.a.
• Molecular weight: 9378.03 Da
• Isoelectric Point: 10.4800

>T162161 WP_000513532.1 NZ_CP055257:11793-12041 [Escherichia coli]
MGKADKLLAKFLNSKKTFEWDELVVLFSSLGYVKKEMQGSRVRFFNAEINHTILMHRPHPESYIKGGTLKAIKQNLKEAG
VL

Nucleotide

• Length: 249 bp

>T162161 NZ_CP071626:2706717-2706908 [Rhizobium leguminosarum]
GTGACGTTTCTCTTTGACGTCAATGTCCTCATTGCCCTTTTTGACCCCCACCATCTTTATCATGAACCTGCGCACCGCTG
GTTCCGCTCCCTTGACCGCGACAGTTGGGCGACCTGTCCGCTGACCGAGAACGGTGTTGCTCGCATTCTCGGTAAATCCA
AACTACCCGGATTCGCCTGGTTCTCCGGCTAA

Antitoxin

Protein

• Length: 116 a.a.
• Molecular weight: 13206.97 Da
• Isoelectric Point: 4.8313

>AT162161 WP_000681609.1 NZ_CP055257:12038-12385 [Escherichia coli]
MKHLKYKGYLGTVEPDFENNILYGKLAFIRDLVTYEAETLADLEREFKTSVDLYLQSCVEDGKEPDTPFKGVFNVRLDPE
LHRRVAEMAMEEDLSLNAFVNKALEKEVSHHRAGA

Nucleotide

• Length: 348 bp

>AT162161 NZ_CP071626:2706493-2706720 [Rhizobium leguminosarum]
ATGAAAACGACACTCGCGATAGATGACGACGTTCTCGACGCAGCCAGAGAGATAGCAGAACGCCAGGAGAAAAATCTTGG
CGAAGTTATTTCCGACCTCGCCCGAAGATCACTGGAGATGCAACAGCTGGCACCTCGGCGTAACGGTGTGCCTCTTATCA
AGAGAAAGCCCACAGATCGAACGATTACGCCTGAATTCGTCAATGAACTCCGTGATGAATATCCGTGA

Structures

Toxin

• AlphaFold DB: A0A1I9ZJC5

Antitoxin

• AlphaFold DB: A0A1I9ZJ49