162160

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) TsxAB/MqsA(antitoxin)
Location 6862..7355 Replicon plasmid pAH25-1
Accession NZ_CP055257
Organism Escherichia coli strain AH25

Toxin (Protein)


Gene name TsxA Uniprot ID U9Y9W8
Locus tag HUU84_RS24735 Protein ID WP_001230709.1
Coordinates 6862..7116 (+) Length 85 a.a.

Antitoxin (Protein)


Gene name TsxB Uniprot ID U9Y4R8
Locus tag HUU84_RS24740 Protein ID WP_000151456.1
Coordinates 7113..7355 (+) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HUU84_RS24700 2431..2592 + 162 WP_000775236.1 type I toxin-antitoxin system Hok family toxin -
HUU84_RS24705 2742..3338 + 597 WP_000125165.1 hypothetical protein -
HUU84_RS24710 3356..3640 - 285 WP_001542021.1 hypothetical protein -
HUU84_RS24715 3719..4384 - 666 WP_000532148.1 P-loop NTPase -
HUU84_RS24720 4974..5303 + 330 WP_001542022.1 hypothetical protein -
HUU84_RS24725 5704..6219 + 516 WP_001025393.1 J domain-containing protein -
HUU84_RS24730 6323..6865 + 543 WP_001083904.1 hypothetical protein -
HUU84_RS24735 6862..7116 + 255 WP_001230709.1 hypothetical protein Toxin
HUU84_RS24740 7113..7355 + 243 WP_000151456.1 hypothetical protein Antitoxin
HUU84_RS24745 7492..7824 + 333 WP_001393253.1 WbuC family cupin fold metalloprotein -
HUU84_RS24750 7871..8746 - 876 WP_015387340.1 class A extended-spectrum beta-lactamase CTX-M-55 -
HUU84_RS24755 8999..10261 - 1263 WP_000608644.1 IS1380-like element ISEc9 family transposase -
HUU84_RS24760 10713..10895 + 183 WP_071599349.1 hypothetical protein -
HUU84_RS24765 11011..11304 + 294 WP_000673428.1 hypothetical protein -
HUU84_RS24770 11793..12041 + 249 WP_000513532.1 type II toxin-antitoxin system HicA family toxin -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Mobilizable plasmid blaCTX-M-55 / mcr-1.1 - 1..64941 64941
- flank IS/Tn blaCTX-M-55 - 7871..10261 2390


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(25-83)

Antitoxin

Uncharacterised protein family HicB

(54-104)


Sequences

Toxin        


Download         Length: 85 a.a.        Molecular weight: 9942.47 Da        Isoelectric Point: 10.1206

>T162160 WP_001230709.1 NZ_CP055257:6862-7116 [Escherichia coli]
MRKNKGRLTYYLEVIDKKYHFVKKISSYSKEFTDGKTKRTKRTLSELVFNESEVEAIDFTKNGLRPVDKNILLTMVKEYK
ESDA

Download         Length: 255 bp

>T162160 NZ_CP071626:c2420921-2420661 [Rhizobium leguminosarum]
ATGACTGTTAGTGGAAAACACCGGACGACTCTGGAAGCCATATTCGCCGATCCCGTCCGCGCCAGCATCGCCTGGCGCGA
TATCGAGGCGCTTTTCGTCGCTTGCGGGGGAGAAGTCAGCGAAGGCAACGGCTCGCGCGTCCGCGTTGCACTGAACGGCG
TTCGCGCGGTTTTCCATCGGCCGCATCCGCAGAAGGAAACAGACAAGGGCGCGGTACGGTCGGTGAGACGTTTTCTGCAG
GAAGCGGAGATAAGACCATGA

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9136.45 Da        Isoelectric Point: 7.2775

>AT162160 WP_000151456.1 NZ_CP055257:7113-7355 [Escherichia coli]
MTIENTPENIKKLRKKIGLTQTECGEIFGVGLSTWQKKEAKTHNQLNLSKGEFEYLLLLAGEHPEYVLHKKAESRSPEEP

Download         Length: 243 bp

>AT162160 NZ_CP071626:c2420664-2420335 [Rhizobium leguminosarum]
ATGAACGTTATGCACTACAAAGGTTATGAGGCCGTCATCGAATTCGATGAAGAGGCAGAGATTTTTCATGGCGAAGTCGT
CAACATGCGTGATGTCATTACCTTTCAGGGAACGTCAGCTGCGGAGCTGAAACAGGCCTTCGCCGATTCGCTGGATGATT
ATCTGGCTTTCTGCGCAGAACGCGGCGAACAGCCGGAAAAGCCGTTTTCAGGGCAGTTCGTGGTCCGCGCTGATCCGACC
CTGCACAAGGCGCTTTCAAGTGCGGCACAACGCGACGGCGTCAGCCTGAACAAATGGGTGACGTCTGCGCTCGAACGCGC
CGTCGGCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A3G9H8F0

Antitoxin

Source ID Structure
AlphaFold DB A0A3G9GNF5

References