Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type V Classification (family/domain) ghoTS/ghoT-GhoS
Location 4654502..4654999 Replicon chromosome
Accession NZ_CP055256
Organism Escherichia coli strain AH25

Toxin (Protein)


Gene name ghoT Uniprot ID L4JJG6
Locus tag HUU84_RS22455 Protein ID WP_001173343.1
Coordinates 4654502..4654675 (-) Length 58 a.a.

Antitoxin (Protein)


Gene name ghoS Uniprot ID L4JK92
Locus tag HUU84_RS22460 Protein ID WP_000398619.1
Coordinates 4654703..4654999 (-) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HUU84_RS22445 4651172..4652629 + 1458 WP_000856832.1 dipeptide/tripeptide permease DtpC -
HUU84_RS22450 4652866..4654383 + 1518 WP_001295074.1 lysine--tRNA ligase -
HUU84_RS22455 4654502..4654675 - 174 WP_001173343.1 type V toxin-antitoxin system toxin GhoT Toxin
HUU84_RS22460 4654703..4654999 - 297 WP_000398619.1 type V toxin-antitoxin system endoribonuclease antitoxin GhoS Antitoxin
HUU84_RS22465 4655226..4655498 - 273 WP_000405647.1 N-acetyltransferase -
HUU84_RS22470 4655510..4655740 - 231 WP_000371700.1 (4Fe-4S)-binding protein -
HUU84_RS22475 4655921..4657552 + 1632 WP_001216487.1 sensor histidine kinase -
HUU84_RS22480 4657549..4658268 + 720 WP_000611288.1 two-component system response regulator DcuR -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - - 4615473..4654999 39526


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin GhoT/OrtT

(4-57)

Antitoxin

Endoribonuclease antitoxin GhoS

(5-94)


Sequences

Toxin        


Download         Length: 58 a.a.        Molecular weight: 6555.06 Da        Isoelectric Point: 10.2288

>T162158 WP_001173343.1 NZ_CP055256:c4654675-4654502 [Escherichia coli]
MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITWPMSLPVALLFSLF

Download         Length: 174 bp

>T162158 NZ_CP071626:804999-805244 [Rhizobium leguminosarum]
ATGAAAAAGATCGCCTACAGCAAGGCCGCCACGAAGGCGCTCCGCAAGATGCAGCCGAAGCGCGCAGCGGCGATCGTCGC
CAAGGTCGAAGCCTTCGCCAACGGCGAGACGGTCGACCTCAAGAAGCTGCAAGGCAGCGATTTTTTTCGAATTCGTGTCG
GGCAGGACCGTGTCATCATCGACGATCAGACGCTGCAGGTCCTCGTCATCAAGGCTGGCCCACGCGGCGACATCGATAAC
GAATAG

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11467.97 Da        Isoelectric Point: 4.1705

>AT162158 WP_000398619.1 NZ_CP055256:c4654999-4654703 [Escherichia coli]
MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEYGIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDI
DFDLNIMTVDDYFRQFYK

Download         Length: 297 bp

>AT162158 NZ_CP071626:805254-805601 [Rhizobium leguminosarum]
ATGGGTGAAATCCAGAAACTGACCATCGACGGCAAGGGGTACGTGCTGCTTTCCGAAGAAGACTATCAGGATCTTGTCGA
CGTCAGCGACGCGCGTGCCGCAAAGGCGCGTATCGACGCTGGCGAAGAGACCTGGCCTGAAGATGTCGTCAAGGCGCTGA
TCGCCGGCGAAGATCCTGTCCGGGTGTTCCGTAAGCATCGCGGCATGAGCATCAAGGAGCTGGCTGAAAAGTCCGGGCTC
TCCCAGCCCTATCTTTCCGAAATCGAGACCGGAAAGAAGGAGGGCTCCCTGGACGCACTCAGGTCGATCGCCAGCGCGCT
GGACGTCGACCTTGACGATGTGGCTTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9QI41

Antitoxin

Source ID Structure
PDB 2LLZ
AlphaFold DB A0A7U9QBH6

References