Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) itaRT/DUF1778(antitoxin)
Location 3773150..3773987 Replicon chromosome
Accession NZ_CP055256
Organism Escherichia coli strain AH25

Toxin (Protein)


Gene name itaT Uniprot ID Q3Z4X7
Locus tag HUU84_RS18280 Protein ID WP_000227784.1
Coordinates 3773445..3773987 (+) Length 181 a.a.

Antitoxin (Protein)


Gene name itaR Uniprot ID I2UQS9
Locus tag HUU84_RS18275 Protein ID WP_001297137.1
Coordinates 3773150..3773461 (+) Length 104 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HUU84_RS18250 3768170..3769117 + 948 WP_001239441.1 cytochrome o ubiquinol oxidase subunit II -
HUU84_RS18255 3769139..3771130 + 1992 WP_000467180.1 cytochrome o ubiquinol oxidase subunit I -
HUU84_RS18260 3771120..3771734 + 615 WP_000179819.1 cytochrome o ubiquinol oxidase subunit III -
HUU84_RS18265 3771734..3772063 + 330 WP_000019869.1 cytochrome o ubiquinol oxidase subunit IV -
HUU84_RS18270 3772075..3772965 + 891 WP_000971332.1 heme o synthase -
HUU84_RS18275 3773150..3773461 + 312 WP_001297137.1 DUF1778 domain-containing protein Antitoxin
HUU84_RS18280 3773445..3773987 + 543 WP_000227784.1 GNAT family N-acetyltransferase Toxin
HUU84_RS18285 3774043..3774978 - 936 WP_001297127.1 sel1 repeat family protein -
HUU84_RS18290 3775386..3776750 + 1365 WP_072643898.1 MFS transporter -
HUU84_RS18295 3776878..3777369 - 492 WP_001138904.1 nucleotide binding protein YajQ -
HUU84_RS18300 3777537..3778448 + 912 WP_000705874.1 2-dehydropantoate 2-reductase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(68-156)

Antitoxin

Vibrio phage ICP1, Orf50

(14-97)


Sequences

Toxin        


Download         Length: 181 a.a.        Molecular weight: 19805.02 Da        Isoelectric Point: 8.3395

>T162153 WP_000227784.1 NZ_CP055256:3773445-3773987 [Escherichia coli]
MVDKHEEITLPIVLSCNYQSDITYPGQKQFDCGNPVIDKFVRASLKKSVRNSDCAAKALIDRQSGELIGICTFTAYSLEK
QRVSGVLQGSQPSEIGVVRLVMLGVARKYQKRGFGQDLLCDFFEHVKIIHQALPIKGVYLDADPAAINFYARLGFVQLSA
TPNAFGAVPMFLAIQHILAA

Download         Length: 543 bp

>T162153 NZ_CP071620:70195-70467 [Rhizobium bangladeshense]
ATGAAGTTGCTCTGGACCCCAAATTCCTGGGAAGAATACGAATACTGGCAGAAAACCGACCAGAAGTTGGTCGAGAAAAT
CAGCGAGCTGCTCAGGGATACCAAAAGGTCGCCCTTTAAAGGTCTTGGCAAGCCAGAGCCGCTGAAGGGAGATCTTTCCG
GATTTTGGTCACGGCGCATCCTTGGAGAGCATCGGCTCGTGTACTGTGTGACAGGCAAGGGTGCCAGTCAGCAGTTGGAA
ATTATCCAGTGCCGATTTCATTACGAAAAATAA

Antitoxin


Download         Length: 104 a.a.        Molecular weight: 11358.04 Da        Isoelectric Point: 10.4825

>AT162153 WP_001297137.1 NZ_CP055256:3773150-3773461 [Escherichia coli]
MPGKTATLPDVDKTLKNARVELKTSPDAKNKLREAAQAVGVDLSAFILSAAMERAESVLDNQRRRELSNQSWELMNQLIA
EPAQPTLALKALMKRKNSDGRQA

Download         Length: 312 bp

>AT162153 NZ_CP071620:69942-70193 [Rhizobium bangladeshense]
ATGGCACATGTTCGTTTGACGGAGTTCCGACAGAACATCGCAACCTATTTTGATCAGGTCCTGTCTTCCCGCGCTCCTCT
GCTCGTGACTCGTCAAGGTTCCGAAGCAATGGTGTTGATTGCCGAAAGCGAGTACGAAAGCATGCAAGAGACTTTGCACC
TGCTCTCGACCCCCGCCAATTCTGCCCGGCTACGCGAAAGTATTGGGCAGCTTCGTAGCGGGCAGGGGATAGAGAAAGAT
CCAACTCTTTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 7BYY

Antitoxin

Source ID Structure
AlphaFold DB A0A0E0Y6B3

References