Detailed information of TA system TA162149

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hipBA/HipA-HipB
• Location: 2614906..2616494
• Replicon: chromosome
• Accession: NZ_CP055256
• Organism: Escherichia coli strain AH25

Toxin (Protein)

• Gene name: hipA
• Uniprot ID: E9TB78
• Locus tag: HUU84_RS12850
• Protein ID: WP_001125466.1
• Coordinates: 2615172..2616494 (+)
• Length: 441 a.a.

Antitoxin (Protein)

• Gene name: hipB
• Uniprot ID: -
• Locus tag: HUU84_RS12845
• Protein ID: WP_001296726.1
• Coordinates: 2614906..2615172 (+)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HUU84_RS12845	2614906..2615172	+	267	WP_001296726.1	type II toxin-antitoxin system antitoxin HipB	Antitoxin
HUU84_RS12850	2615172..2616494	+	1323	WP_001125466.1	type II toxin-antitoxin system serine/threonine protein kinase toxin HipA	Toxin
HUU84_RS12855	2616806..2617014	+	209	Protein_2514	helix-turn-helix transcriptional regulator	-
HUU84_RS12860	2617448..2618011	+	564	WP_001296758.1	type 1 fimbrial major subunit FimA	-
HUU84_RS12865	2618372..2619082	+	711	WP_001195166.1	fimbria/pilus periplasmic chaperone	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	fimA	2614906..2630995	16089

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HipA, N-terminal subdomain 1 (4-103)

Antitoxin

• Cro/C1-type helix-turn-helix domain (14-69)

Sequences

Toxin

Protein

• Length: 441 a.a.
• Molecular weight: 49438.05 Da
• Isoelectric Point: 9.1005

>T162149 WP_001125466.1 NZ_CP055256:2615172-2616494 [Escherichia coli]
MPKLVTWMNNQRVGELTKLANGAHTFKYAPEWLASRYARPLSLSLPLQRGNITSDAVFNFFDNLLPDSPIVRDRIVKRYH
AKSRQPFDLLSEIGRDSVGAVTLLPENETITRPIMAWEKLTEARLEEVLTAYKADIPLGMIREENDFRISVAGAQEKTAL
LRIGNDWCIPKGITPTTHIIKLPIGEIRQPNATLDLSQSVDNEYYCLLLAKELGLNVPDAEIIKAGRVRALAVERFDRRW
NTERTVLLRLPQEDMCQTFGLPSSVKYESDGGPGIARIMAFLMGSSEALRDRYDFMKFQVFQWLIGATDGHAKNFSVFIQ
AGGSYRLTPFYDIISAFPVLGGTGIHISDLKLAMGLNASKGKKTAIDKIYPRHYLATAKVLRFPEVQMHEILSDFARMIP
AALDNVKTSLPTDFPENVVTAVETNVLRLHGRLSREYGSK

Nucleotide

• Length: 1323 bp

>T162149 NZ_CP071614:70203-70475 [Rhizobium bangladeshense]
ATGAAGTTGCTCTGGACCCCAAATTCCTGGGAAGAATACGAATACTGGCAGAAAACCGACCAGAAGTTGGTCGAGAAAAT
CAACGAGCTGCTCAGGGATACCAAAAGGTCGCCCTTTAAAGGTCTTGGCAAGCCAGAGCCGCTGAAGGGAGATCTTTCCG
GATTTTGGTCACGGCGCATCCTTGGAGAGCATCGGCTCGTGTACTGTGTGACAGGCAAGGGTGCCAGTCAGCAGTTGGAA
ATTATCCAGTGCCGATTTCATTACGAAAAATAA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 10047.39 Da
• Isoelectric Point: 4.9632

>AT162149 WP_001296726.1 NZ_CP055256:2614906-2615172 [Escherichia coli]
MMSFQKIYSPTQLANAMKLVRQQNGWTQSELAKKIGIKQATISNFENNPDNTTLTTFFKILQSLELSMTLCDTKNASPES
TEQQDLEW

Nucleotide

• Length: 267 bp

>AT162149 NZ_CP071614:69950-70201 [Rhizobium bangladeshense]
ATGGCACATGTTCGTTTGACGGAGTTCCGACAGAACATCGCAACCTATTTTGATCAGGTCCTGTCTTCCCGCGCTCCTCT
GCTCGTGACTCGTCAAGGTTCGGAAGCAATGGTGTTGATTGCCGAAAGCGAGTACGAAAGCATGCAAGAGACTTTGCACC
TGCTCTCGACCCCCGCCAATTCTGCCCGGCTACGCGAAAGTATTGGGCAGCTTCGTAGCGGGCAGGGGATAGAGAAAGAT
CCAACTCTTTGA