Detailed information of TA system TA162140

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 1956009..1956840
• Replicon: chromosome
• Accession: NZ_CP055256
• Organism: Escherichia coli strain AH25

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: S1P968
• Locus tag: HUU84_RS09475
• Protein ID: WP_000854814.1
• Coordinates: 1956009..1956383 (-)
• Length: 125 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: B3Y195
• Locus tag: HUU84_RS09480
• Protein ID: WP_001285585.1
• Coordinates: 1956472..1956840 (-)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HUU84_RS09435	1951405..1952571	+	1167	WP_000830163.1	serine-type D-Ala-D-Ala carboxypeptidase DacD	-
HUU84_RS09440	1952690..1953163	+	474	WP_001105393.1	DNA gyrase inhibitor SbmC	-
HUU84_RS09445	1953361..1954419	+	1059	WP_001200905.1	FUSC family protein	-
HUU84_RS09450	1954591..1954920	+	330	WP_000450409.1	DUF496 family protein	-
HUU84_RS09455	1955021..1955203	-	183	WP_001016348.1	hypothetical protein	-
HUU84_RS09460	1955275..1955403	+	129	Protein_1852	transposase domain-containing protein	-
HUU84_RS09465	1955692..1955805	-	114	WP_001161660.1	DUF957 domain-containing protein	-
HUU84_RS09470	1955818..1956012	-	195	WP_000988600.1	hypothetical protein	-
HUU84_RS09475	1956009..1956383	-	375	WP_000854814.1	type IV toxin-antitoxin system toxin CbtA	Toxin
HUU84_RS09480	1956472..1956840	-	369	WP_001285585.1	type IV toxin-antitoxin system toxin CbtA	Antitoxin
HUU84_RS09485	1956914..1957135	-	222	WP_000692323.1	DUF987 domain-containing protein	-
HUU84_RS09490	1957198..1957674	-	477	WP_001186773.1	RadC family protein	-
HUU84_RS09495	1957690..1958169	-	480	WP_000860076.1	antirestriction protein	-
HUU84_RS09500	1958251..1959072	-	822	WP_001234530.1	DUF945 domain-containing protein	-
HUU84_RS09505	1959293..1959703	-	411	WP_000846713.1	hypothetical protein	-
HUU84_RS09510	1959719..1960401	-	683	Protein_1862	hypothetical protein	-
HUU84_RS09515	1960537..1961607	-	1071	WP_000102643.1	patatin-like phospholipase family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (18-117)

Sequences

Toxin

Protein

• Length: 125 a.a.
• Molecular weight: 13898.94 Da
• Isoelectric Point: 7.8522

>T162140 WP_000854814.1 NZ_CP055256:c1956383-1956009 [Escherichia coli]
MKTLPVLPGQAASSRPSPVEIWQILLSRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SACTRSQLINSIDILRARRATGLMTRDNYRTVNNITLGKYPEAK

Nucleotide

• Length: 375 bp

>T162140 NZ_CP071602:2519767-2519949 [Bordetella parapertussis]
ATGAACAGCACAGACCTGATCCAGCAACCGAAGGCCGACGGCTGGTATCGCGTGCATACCGTGGGTTCACACCACCAGTA
CAAGCACCCGACCAAGCCCGGCAAGGTCACCGTGCCGCATCCCAGGAAGGGCCTGCCGACAGCGACACAGCGCAGCATCC
TTAAGCAAGCCGGCCTGCGATAA

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 13593.48 Da
• Isoelectric Point: 6.3139

>AT162140 WP_001285585.1 NZ_CP055256:c1956840-1956472 [Escherichia coli]
VSDTLPGTTLPDDNHDRPWWGLPCTVTPCFGARLVQEGNRLHYLADRAGIRGLFSDADAYHLDQAFPLLMKQLELMLTSG
ELNPRHQHTVTLYAKGLTCKADTLSSCGYVYLAVYPTPEMKN

Nucleotide

• Length: 369 bp

>AT162140 NZ_CP071602:2519972-2520361 [Bordetella parapertussis]
ATGCTCTACCCGATCCATGTTTCCAAAGACGAAGGCAGCGCCTACGGGGCCGCCTTCCCTGATTTTCCCGGCTGCTTTGC
AGCCGCCGATGAACTCCAGGACCTCCCCCGCGCGGCGCAGGAAGCCGTTGAGGCGCACTTCTACGGAGAGACCGAGCGTA
TCCCCGCCCCGTCCGCCCCTGAAGCGTGGGCCAACGATGAGGACTATCAAGGCGGCTATTGGATGATGGTCGATATCGAC
CTGTCGAAGGTCAACACGAAGGCCGTGCGGCTCAACATCAGCCTGCCGGAAAACCTGGTTCACCGTATCGACGAGGAAGC
CAAGGCACGACGCCTTTCGCGCTCAGCGTTTCTGGCCATGGCTGCGGAGCACGAAATGGCCGACGCCTAG

Structures

Toxin

• AlphaFold DB: A0A829FY50

Antitoxin

• AlphaFold DB: A0A7U9LW60