Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 952354..953008 Replicon chromosome
Accession NZ_CP055256
Organism Escherichia coli strain AH25

Toxin (Protein)


Gene name cptA Uniprot ID F4NJ21
Locus tag HUU84_RS04725 Protein ID WP_000244772.1
Coordinates 952601..953008 (+) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag HUU84_RS04720 Protein ID WP_000354046.1
Coordinates 952354..952620 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HUU84_RS04695 947523..948266 + 744 WP_000951941.1 SDR family oxidoreductase -
HUU84_RS04700 948323..949756 - 1434 WP_001307385.1 6-phospho-beta-glucosidase BglA -
HUU84_RS04705 949801..950112 + 312 WP_001182945.1 N(4)-acetylcytidine aminohydrolase -
HUU84_RS04710 950276..950935 + 660 WP_000250274.1 hemolysin III family protein -
HUU84_RS04715 951131..952111 - 981 WP_000886095.1 tRNA-modifying protein YgfZ -
HUU84_RS04720 952354..952620 + 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
HUU84_RS04725 952601..953008 + 408 WP_000244772.1 protein YgfX Toxin
HUU84_RS04730 953048..953569 - 522 WP_001055874.1 flavodoxin FldB -
HUU84_RS04735 953681..954577 + 897 WP_000806637.1 site-specific tyrosine recombinase XerD -
HUU84_RS04740 954602..955312 + 711 WP_000715208.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
HUU84_RS04745 955318..957051 + 1734 WP_000813220.1 single-stranded-DNA-specific exonuclease RecJ -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16048.02 Da        Isoelectric Point: 11.2511

>T162136 WP_000244772.1 NZ_CP055256:952601-953008 [Escherichia coli]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWCIVKAPWMIKSGMMLRLRSDGGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T162136 NZ_CP071601:1974543-1974794 [Bordetella pertussis]
ATTGGAGGCGCGGCGACAAAAAAGCCGGGACAAAAGCGTCGCGCCGGGGCGGCGGCGCTGGGGCTGGATTTCAGCGCCAT
GGTCGAAATCGTGCTTGCATTGACACCGCGGGATTTCCACAAAAGCATGACTACCCATGCCGACCATCGCATTTGGCAGG
ACGTCTATTGCCCGGTGACAGCGATGGGCGCCGTCTACCTGAAGCTGACGGTCCTCGACGGCGTGCCGATCGTGTCGTTC
AAGGAGTTGTGA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT162136 WP_000354046.1 NZ_CP055256:952354-952620 [Escherichia coli]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT162136 NZ_CP071601:1974797-1975198 [Bordetella pertussis]
ATGAAGTGTCCCGTATGCGGAGCGGCCGCGCTGGCGCGTGATACTCGCGATGTGCCCTATAGCTACAAGGGCGAGACCAT
CACGATTGCGGCGGTGACGGGCGACTGGTGTCCGGCATGCGGCGAGAACGTGCTCGATGGCGCTGAATCGGCGCGGGTGA
GCGAGGCCATGCTGGCTTTCAACCGCCTGGTCAACGCCCGCCGCGTCGACCCCGGGTTCATCGTGCGGGTGCGCAGGAAG
CTGGGGCTGGACCAGCGCGAAGCCGCGGCGCTGTTCGGCGGCGGCACCAATGCGTTCTCGCGTTACGAGAACGGCAAGAC
GCGCCCGCCGCTGGCGCTGGTGAAGCTGCTCAGGCTGCTGGATCGCCATCCGGAGCTGCTGGAGGAAGTCCGTGCGGATT
GA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0E0XYB4

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References