Detailed information of TA system TA162117

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/ParE-DnaT
• Location: 54271..54793
• Replicon: plasmid pAH01-2
• Accession: NZ_CP055253
• Organism: Escherichia coli strain AH01

Toxin (Protein)

• Gene name: relE
• Uniprot ID: U9YLU1
• Locus tag: HUU83_RS24145
• Protein ID: WP_000638823.1
• Coordinates: 54271..54552 (-)
• Length: 94 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: U9Y7J1
• Locus tag: HUU83_RS24150
• Protein ID: WP_000121741.1
• Coordinates: 54542..54793 (-)
• Length: 84 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HUU83_RS24110	50420..50740	-	321	WP_000362080.1	VirB3 family type IV secretion system protein	-
HUU83_RS24115	50811..50999	-	189	WP_050567133.1	hypothetical protein	-
HUU83_RS24120	51101..51685	-	585	WP_001177113.1	lytic transglycosylase domain-containing protein	-
HUU83_RS24125	51706..52104	-	399	WP_001153665.1	hypothetical protein	-
HUU83_RS24130	52223..52660	-	438	WP_000539665.1	type IV pilus biogenesis protein PilM	-
HUU83_RS24135	52666..53901	-	1236	WP_072652802.1	TcpQ domain-containing protein	-
HUU83_RS24140	53904..54203	-	300	WP_000835764.1	IncN plasmid KikA protein	-
HUU83_RS24145	54271..54552	-	282	WP_000638823.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
HUU83_RS24150	54542..54793	-	252	WP_000121741.1	hypothetical protein	Antitoxin
HUU83_RS24155	54893..55528	-	636	WP_015059536.1	hypothetical protein	-
HUU83_RS24160	55576..56385	+	810	WP_171004827.1	DNA primase	-
HUU83_RS24165	56608..56832	-	225	WP_000713562.1	EexN family lipoprotein	-
HUU83_RS24170	56841..57485	-	645	WP_001310442.1	conjugal transfer protein TrbJ	-
HUU83_RS24175	57491..58486	-	996	WP_065304446.1	type IV secretion system protein	-
HUU83_RS24180	58490..58747	-	258	WP_000739144.1	hypothetical protein	-
HUU83_RS24185	58744..59046	-	303	WP_001360345.1	hypothetical protein	-
HUU83_RS24190	59027..59284	-	258	WP_001542015.1	hypothetical protein	-
HUU83_RS24195	59317..59763	-	447	WP_001243165.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	mcr-1.1	-	1..64771	64771

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (5-87)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 94 a.a.
• Molecular weight: 10945.79 Da
• Isoelectric Point: 10.4997

>T162117 WP_000638823.1 NZ_CP055253:c54552-54271 [Escherichia coli]
MIYELAFDHRALKEWQKLGHTVREQFKKKLSERLINPRVPAAKLHGHTDRYKIKLRASGYRLVYQVIDEKVVLLVISVGK
REGSDVYHMADTR

Nucleotide

• Length: 282 bp

>T162117 NZ_CP071586:6155890-6156186 [Pseudomonas germanica]
ATGACCTATAACCTTGAATTTGATCGTCGTGCTTTAAAGGAGTGGAACAAACTCGGCGATACGGTTCGCCACCAGTTCAA
GAAGAAGCTCACAGAGGTATTGGAGAATCCTCGAATAGAAGCCAATCGACTTCGCGAGCTCCCGGATTGCTACAAAGTGA
AGCTGAAAAGCGCTGGCTATCGCCTGATCTATCAGGTCCTTGATCAAGAGATTGTGGTGTTTGTAATCGCAATCGGGAAG
CGAGAGAGAGAAGCTGCTTACGAGGTTGCGCAGGACCGTCTTTCGCTCCTGCCCTGA

Antitoxin

Protein

• Length: 84 a.a.
• Molecular weight: 9223.38 Da
• Isoelectric Point: 3.9823

>AT162117 WP_000121741.1 NZ_CP055253:c54793-54542 [Escherichia coli]
MSYQILTTTAASITDLKKNPMGTVAEGEGDAVAILNRNEPAFYCVPPELYAYYRELAEDAELNAIADERMKNPDIVRVNL
DDL

Nucleotide

• Length: 252 bp

>AT162117 NZ_CP071586:6155661-6155900 [Pseudomonas germanica]
ATGGCCTCCATCAACATTCGCATCGACGATGATCTGAAGCAGCGCTCCTTCGCCGAACTGGAAAAGCTGGGAGTAACCCC
CTCAGAGCTTTTGCGTCAGACACTCCAATACGTCGCCGAGCGAGGCAAGCTACCCTTCAAAGCAGCATTGCTCAGCGAGG
AAGATGAAGTACTCATAGCCGTAGTCACCGAACGCCTCGCGGCACCTCAACGAGTCAAGGTAAGTCTGGATGACCTATAA

Structures

Toxin

• AlphaFold DB: U9YLU1

Antitoxin

• AlphaFold DB: U9Y7J1