Detailed information of TA system TA162116

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/-
• Location: 10725..11317
• Replicon: plasmid pAH01-2
• Accession: NZ_CP055253
• Organism: Escherichia coli strain AH01

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: A0A1I9ZJC5
• Locus tag: HUU83_RS23910
• Protein ID: WP_000513532.1
• Coordinates: 10725..10973 (+)
• Length: 83 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: A0A1I9ZJ49
• Locus tag: HUU83_RS23915
• Protein ID: WP_000681609.1
• Coordinates: 10970..11317 (+)
• Length: 116 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HUU83_RS23875	5729..6394	-	666	WP_000532148.1	P-loop NTPase	-
HUU83_RS23880	6983..7312	+	330	WP_000855119.1	hypothetical protein	-
HUU83_RS23885	7716..8231	+	516	WP_001025393.1	J domain-containing protein	-
HUU83_RS23890	8335..8877	+	543	WP_001083904.1	hypothetical protein	-
HUU83_RS23895	8874..9128	+	255	WP_001230709.1	hypothetical protein	-
HUU83_RS23900	9125..9367	+	243	WP_000151456.1	hypothetical protein	-
HUU83_RS23905	9938..10231	+	294	WP_000673428.1	hypothetical protein	-
HUU83_RS23910	10725..10973	+	249	WP_000513532.1	type II toxin-antitoxin system HicA family toxin	Toxin
HUU83_RS23915	10970..11317	+	348	WP_000681609.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
HUU83_RS23920	11405..11587	-	183	WP_001428470.1	hypothetical protein	-
HUU83_RS23925	12089..12388	+	300	WP_034169410.1	type II toxin-antitoxin system HicB family antitoxin	-
HUU83_RS23930	12648..12974	-	327	WP_000206697.1	hypothetical protein	-
HUU83_RS23935	12976..13416	-	441	WP_023223352.1	hypothetical protein	-
HUU83_RS23940	13436..14143	-	708	WP_063084541.1	hypothetical protein	-
HUU83_RS23945	14145..14897	-	753	WP_000797967.1	conjugal transfer protein TraL	-
HUU83_RS23950	14915..15316	-	402	WP_063084542.1	hypothetical protein	-
HUU83_RS23955	15684..16025	+	342	WP_000730722.1	plasmid mobilization relaxosome protein MobC	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	mcr-1.1	-	1..64771	64771

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (20-81)

Antitoxin

• Uncharacterised protein family HicB (54-104)

Sequences

Toxin

Protein

• Length: 83 a.a.
• Molecular weight: 9378.03 Da
• Isoelectric Point: 10.4800

>T162116 WP_000513532.1 NZ_CP055253:10725-10973 [Escherichia coli]
MGKADKLLAKFLNSKKTFEWDELVVLFSSLGYVKKEMQGSRVRFFNAEINHTILMHRPHPESYIKGGTLKAIKQNLKEAG
VL

Nucleotide

• Length: 249 bp

>T162116 NZ_CP071586:c5761814-5761470 [Pseudomonas germanica]
ATGCGAACCGTATTCTTTGAAACAACGATATTTACCACAAGCGTTGGTCATTACCTGACAGACGATGAGTACCGAGAACT
GCAGGCTTATATGCAGGGCAATCCGTTGGCAGGCGATGTGATGCCCCGAACGGGTGGATTCAGAAAACTACGTTGGGCTG
ATGCTCGACGTGGCAAGGGCCGACGAGGCGGCTTGCGGGTCATCTATTATTGGCTTATGAATGATGGTCAGTTCTGGATG
TTTGCAATTTACGACAAAGATGGGCTTGAGAATCTGACTGCCGAGCAAGAAAAGGCGCTCAAAAAAGCCATAGATAAAGA
GTTGAAAACACGAGGTACCCCATGA

Antitoxin

Protein

• Length: 116 a.a.
• Molecular weight: 13206.97 Da
• Isoelectric Point: 4.8313

>AT162116 WP_000681609.1 NZ_CP055253:10970-11317 [Escherichia coli]
MKHLKYKGYLGTVEPDFENNILYGKLAFIRDLVTYEAETLADLEREFKTSVDLYLQSCVEDGKEPDTPFKGVFNVRLDPE
LHRRVAEMAMEEDLSLNAFVNKALEKEVSHHRAGA

Nucleotide

• Length: 348 bp

>AT162116 NZ_CP071586:c5761473-5761159 [Pseudomonas germanica]
ATGAAAAAACGCGACCTGTTTGCAGAGTTGATGCAGGGCGTTGATGAGATGGCGGCTCAACGCGAAGGAAAAATTACCCT
GCGCAATACAGTGCTTGAGGACAAACCAGTCCCGGAAGTCGGAGCCCAAGAAATCGTGGCTTTGAGAGAAAAGTTACATA
TGTCTCAAGCCGTCTTTGCCAAGCGAATCAGGGCGAGTCCCAGCACTCTGAGAAACTGGGAGCAGGAAAAGTCAAAGCCA
AATGCTCAAGCTGCTTTGTTGATCCGTTTGGTTGAAAAATTCCCTGACATGGTTGACCGCCTGAGCGCCGTTTAA

Structures

Toxin

• AlphaFold DB: A0A1I9ZJC5

Antitoxin

• AlphaFold DB: A0A1I9ZJ49