Detailed information of TA system TA162115

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): TsxAB/MqsA(antitoxin)
• Location: 8874..9367
• Replicon: plasmid pAH01-2
• Accession: NZ_CP055253
• Organism: Escherichia coli strain AH01

Toxin (Protein)

• Gene name: TsxA
• Uniprot ID: U9Y9W8
• Locus tag: HUU83_RS23895
• Protein ID: WP_001230709.1
• Coordinates: 8874..9128 (+)
• Length: 85 a.a.

Antitoxin (Protein)

• Gene name: TsxB
• Uniprot ID: U9Y4R8
• Locus tag: HUU83_RS23900
• Protein ID: WP_000151456.1
• Coordinates: 9125..9367 (+)
• Length: 81 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HUU83_RS23860	4446..4607	+	162	WP_000775236.1	type I toxin-antitoxin system Hok family toxin	-
HUU83_RS23865	4757..5353	+	597	WP_000125165.1	hypothetical protein	-
HUU83_RS23870	5371..5541	-	171	WP_000186151.1	hypothetical protein	-
HUU83_RS23875	5729..6394	-	666	WP_000532148.1	P-loop NTPase	-
HUU83_RS23880	6983..7312	+	330	WP_000855119.1	hypothetical protein	-
HUU83_RS23885	7716..8231	+	516	WP_001025393.1	J domain-containing protein	-
HUU83_RS23890	8335..8877	+	543	WP_001083904.1	hypothetical protein	-
HUU83_RS23895	8874..9128	+	255	WP_001230709.1	hypothetical protein	Toxin
HUU83_RS23900	9125..9367	+	243	WP_000151456.1	hypothetical protein	Antitoxin
HUU83_RS23905	9938..10231	+	294	WP_000673428.1	hypothetical protein	-
HUU83_RS23910	10725..10973	+	249	WP_000513532.1	type II toxin-antitoxin system HicA family toxin	-
HUU83_RS23915	10970..11317	+	348	WP_000681609.1	type II toxin-antitoxin system HicB family antitoxin	-
HUU83_RS23920	11405..11587	-	183	WP_001428470.1	hypothetical protein	-
HUU83_RS23925	12089..12388	+	300	WP_034169410.1	type II toxin-antitoxin system HicB family antitoxin	-
HUU83_RS23930	12648..12974	-	327	WP_000206697.1	hypothetical protein	-
HUU83_RS23935	12976..13416	-	441	WP_023223352.1	hypothetical protein	-
HUU83_RS23940	13436..14143	-	708	WP_063084541.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	mcr-1.1	-	1..64771	64771

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (15-107)

Antitoxin

• HigA2-like, helix-turn-helix domain (11-90)

Sequences

Toxin

Protein

• Length: 85 a.a.
• Molecular weight: 9942.47 Da
• Isoelectric Point: 10.1206

>T162115 WP_001230709.1 NZ_CP055253:8874-9128 [Escherichia coli]
MRKNKGRLTYYLEVIDKKYHFVKKISSYSKEFTDGKTKRTKRTLSELVFNESEVEAIDFTKNGLRPVDKNILLTMVKEYK
ESDA

Nucleotide

• Length: 255 bp

>T162115 NZ_CP071586:5358728-5359096 [Pseudomonas germanica]
ATGAGTGCAAGGAAGCCGCTCTTGTGGGTCTGCAGTAGCAAGAAAGATCTCAAGCAGATGCCCACAGACGTACAGGATGT
TTTTGGTTATGCACTGGATTTGGCCCAGATTGGTTTGAAGCATCCGGATGCAAAACCCCTGAAAGGGTTTGGAGGCGCAG
GTGTACTGGAGCTGATCGAAAACTTCGACACCAACACCTACCGTGCCATCTATACGGTTCGTTTCGGCAGTGCCATTTAT
GTATTGCACTGCTTTCAAAAGAAGTCGACTACCGGAATCAAAACGGCTCAGTCCGATGTTGATCTGATACGAAACCGCCT
TCGAGCCGCTCAGGATCACGCGAAAGGATCAGAAAATGATAGAAATTGA

Antitoxin

Protein

• Length: 81 a.a.
• Molecular weight: 9136.45 Da
• Isoelectric Point: 7.2775

>AT162115 WP_000151456.1 NZ_CP055253:9125-9367 [Escherichia coli]
MTIENTPENIKKLRKKIGLTQTECGEIFGVGLSTWQKKEAKTHNQLNLSKGEFEYLLLLAGEHPEYVLHKKAESRSPEEP

Nucleotide

• Length: 243 bp

>AT162115 NZ_CP071586:5359083-5359403 [Pseudomonas germanica]
ATGATAGAAATTGAAAAGGGTAGCGGGAACGTCTACCAAGACCTCGAACTGCCCAATGCCAGTGAAATGCAGGTTAAGTC
GCAGCTCGCGGCGAAGATCGGCGAGATCATCAAGGCTCGCCATCTCACTCAGATTCAGGCGTCTGAAATCCTTGGATTAT
CCCAGCCAAAACTTTCCGAAATGCTGCGGGGTAAATTTCGAGGGATCAGTGAAGCCAAAATGATGGAATGTCTTTCGCAT
CTTGGGCGAGACGTGCAGATCGTAGTGAAATCGGCCCCCCGATCACGGAAAGAAGGCCGAATTGAAGTGGTGTTTGCTTA
A

Structures

Toxin

• AlphaFold DB: A0A3G9H8F0

Antitoxin

• AlphaFold DB: A0A3G9GNF5