Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/-
Location 4367831..4368462 Replicon chromosome
Accession NZ_CP055251
Organism Escherichia coli strain AH01

Toxin (Protein)


Gene name hicA Uniprot ID F4NNK6
Locus tag HUU83_RS21160 Protein ID WP_001259388.1
Coordinates 4368187..4368462 (-) Length 92 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID U9Y7E1
Locus tag HUU83_RS21155 Protein ID WP_000593555.1
Coordinates 4367831..4368190 (-) Length 120 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HUU83_RS21130 4363963..4364907 + 945 WP_000947068.1 nickel ABC transporter permease subunit NikB -
HUU83_RS21135 4364904..4365737 + 834 WP_001008963.1 nickel ABC transporter permease subunit NikC -
HUU83_RS21140 4365737..4366501 + 765 WP_001136236.1 nickel import ATP-binding protein NikD -
HUU83_RS21145 4366498..4367304 + 807 WP_124783472.1 nickel import ATP-binding protein NikE -
HUU83_RS21150 4367310..4367711 + 402 WP_001190062.1 nickel-responsive transcriptional regulator NikR -
HUU83_RS21155 4367831..4368190 - 360 WP_000593555.1 type II toxin-antitoxin system HicB family antitoxin Antitoxin
HUU83_RS21160 4368187..4368462 - 276 WP_001259388.1 type II toxin-antitoxin system HicA family toxin Toxin
HUU83_RS21165 4368535..4369659 - 1125 WP_001314210.1 ABC transporter permease -
HUU83_RS21170 4369659..4372394 - 2736 WP_000149125.1 ribosome-associated ATPase/putative transporter RbbA -
HUU83_RS21175 4372391..4373458 - 1068 WP_000361485.1 HlyD family secretion protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(30-80)

Antitoxin

Uncharacterised protein family HicB

(60-112)


Sequences

Toxin        


Download         Length: 92 a.a.        Molecular weight: 10151.89 Da        Isoelectric Point: 10.7076

>T162113 WP_001259388.1 NZ_CP055251:c4368462-4368187 [Escherichia coli]
MRTKVQALRKKQKNTLDQIVKTPVPQGIKWSDIESLVKALGGEIKEGRGSRCKFILNMSVACFHRPHPSPDTDKGAVESV
RDWLLSIGVKP

Download         Length: 276 bp

>T162113 NZ_CP071586:c2687333-2687049 [Pseudomonas germanica]
ATGCAGATTAAGTGGCGACCCAAGGCTCGGGTCGAACTCTCGAAAATCCTGAAGTACATTGGCGAGCGAAATTTTGTAGC
GGCGACTGCACTTCACAAGTCCGTCGTAAAAACTACATCTGCGCTTTGTTGGCATCCACACCTGTATCGCAAAGGCCGCT
CTCCCGGCACTCGAGAGATTGTGGTAAGCCCGAACTATCTAGTCGTTTATCAAGTGGCGGACCAAATTGAGATTGTGAGC
ATCTTGCATGCGCGTCAGGAGTACCCTCGACGCGACCCCGGTTAA

Antitoxin


Download         Length: 120 a.a.        Molecular weight: 13402.27 Da        Isoelectric Point: 5.1987

>AT162113 WP_000593555.1 NZ_CP055251:c4368190-4367831 [Escherichia coli]
MIKLKTPNSMEIAGQPAVITYVPELNAFRGKFLGLSGYCDFVSDSIQGLQKEGELSLREYLEDCKAAGIEPYARTEKIKT
FTLRYPESLSERLNNAAAQQQVSVNTYIIETLNERLNHL

Download         Length: 360 bp

>AT162113 NZ_CP071586:c2687529-2687323 [Pseudomonas germanica]
ATGATCGACCCGTCCTTGATCGACCCACTGTGCCCGGTTGTTTCGGATCTTGATCCCGAGGAACAGGCTGCAAGTTATGA
CCGTTGGTTTCGGGCCGAAGTACAGGCCTCTCTCGATGATCCGCGCCCGAGTATTCCCCATGAGCAAGTGATGGAGGAAA
TCAACGCCTTGATGGAATCAATGCGCAAAAGCGCTGATGCAGATTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0A0FFA2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9LKZ6

References