Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 3741319..3741973 Replicon chromosome
Accession NZ_CP055251
Organism Escherichia coli strain AH01

Toxin (Protein)


Gene name cptA Uniprot ID F4NJ21
Locus tag HUU83_RS18010 Protein ID WP_000244772.1
Coordinates 3741319..3741726 (-) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag HUU83_RS18015 Protein ID WP_000354046.1
Coordinates 3741707..3741973 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HUU83_RS17990 3737276..3739009 - 1734 WP_000813210.1 single-stranded-DNA-specific exonuclease RecJ -
HUU83_RS17995 3739015..3739725 - 711 WP_000715214.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
HUU83_RS18000 3739750..3740646 - 897 WP_000806638.1 site-specific tyrosine recombinase XerD -
HUU83_RS18005 3740758..3741279 + 522 WP_001055874.1 flavodoxin FldB -
HUU83_RS18010 3741319..3741726 - 408 WP_000244772.1 protein YgfX Toxin
HUU83_RS18015 3741707..3741973 - 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
HUU83_RS18020 3742216..3743196 + 981 WP_001472148.1 tRNA-modifying protein YgfZ -
HUU83_RS18025 3743273..3743932 - 660 WP_000250274.1 hemolysin III family protein -
HUU83_RS18030 3744096..3744407 - 312 WP_001182944.1 N(4)-acetylcytidine aminohydrolase -
HUU83_RS18035 3744452..3745885 + 1434 WP_001307385.1 6-phospho-beta-glucosidase BglA -
HUU83_RS18040 3745942..3746685 - 744 WP_000951964.1 SDR family oxidoreductase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16048.02 Da        Isoelectric Point: 11.2511

>T162110 WP_000244772.1 NZ_CP055251:c3741726-3741319 [Escherichia coli]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWCIVKAPWMIKSGMMLRLRSDGGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T162110 NZ_CP071586:c956363-956073 [Pseudomonas germanica]
ATGACCGAAGAATTCACCCGTTTTGACGCCGTCGAGTACCTTAAGACTCCCGAGGATATGGCGGCATATCTGGATGCCTG
TTTTGACGACGATGAAGGCGATGGCGTTCTGATTCGTGCAGCACTCAATGACATCGCCCGAGCTCAAGGCATGACACAAG
TGGCGCGGGATGCAGGTTTGGGCCGCGAAAGTCTTTATAAAGCGCTCAGTAGCACTGGCAATCCCGAATTCGCAACTATC
ATGAAAGTCATGAAGGCTCTGGGTTTGCGATTGCATGCCGTAACACTGTGA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT162110 WP_000354046.1 NZ_CP055251:c3741973-3741707 [Escherichia coli]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT162110 NZ_CP071586:c956669-956367 [Pseudomonas germanica]
ATGATCTCTTTTGAACGATCCCGCATATTTTCCGAATGGCTCGACTCCTTAAAAGACATCAAAGGGAAAGGCCGAATCAT
CTCCAGGATTAAAACAGCCGAACATGGCAACTTCGGCGATTGCGGCTTCGTCGGTGACACCGTTTATGAAATGCGTGTTC
ATTACGGCCCTGGTTACAGAATGTACTTCACACGTAAAGGCGAAGTGATTTATCTGCTGTTGATCGGGGGCGACAAATCG
ACACAAAAGAGAGATATCAAACGCGCCGTGCAAATGGCTCATAACATCGGAAATGAGGAGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0E0XYB4

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References