Detailed information of TA system TA162108

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VagC
• Location: 3309495..3310120
• Replicon: chromosome
• Accession: NZ_CP055251
• Organism: Escherichia coli strain AH01

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: HUU83_RS15960
• Protein ID: WP_000911330.1
• Coordinates: 3309495..3309893 (-)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: U9XQV3
• Locus tag: HUU83_RS15965
• Protein ID: WP_000450524.1
• Coordinates: 3309893..3310120 (-)
• Length: 76 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HUU83_RS15940	3305373..3305573	+	201	WP_000383836.1	YpfN family protein	-
HUU83_RS15945	3305683..3306381	-	699	WP_000679823.1	esterase	-
HUU83_RS15950	3306455..3308470	-	2016	WP_000829294.1	tRNA cytosine(34) acetyltransferase TmcA	-
HUU83_RS15955	3308485..3309348	-	864	WP_001267498.1	neutral zinc metallopeptidase	-
HUU83_RS15960	3309495..3309893	-	399	WP_000911330.1	tRNA(fMet)-specific endonuclease VapC	Toxin
HUU83_RS15965	3309893..3310120	-	228	WP_000450524.1	toxin-antitoxin system antitoxin VapB	Antitoxin
HUU83_RS15970	3310274..3310987	-	714	WP_001295467.1	phosphoribosylaminoimidazolesuccinocarboxamide synthase	-
HUU83_RS15975	3311200..3312234	-	1035	WP_001330579.1	outer membrane protein assembly factor BamC	-
HUU83_RS15980	3312251..3313129	-	879	WP_001295469.1	4-hydroxy-tetrahydrodipicolinate synthase	-
HUU83_RS15985	3313275..3313847	+	573	WP_000176187.1	glycine cleavage system transcriptional repressor	-
HUU83_RS15990	3313847..3314317	+	471	WP_001068682.1	thioredoxin-dependent thiol peroxidase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-125)

Antitoxin

• SpoVT-AbrB domain (7-52)

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 14894.25 Da
• Isoelectric Point: 9.2216

>T162108 WP_000911330.1 NZ_CP055251:c3309893-3309495 [Escherichia coli]
MLKFMLDTNICIFTIKNKPVHVRERFNLNSGRMCISSVTLMELIYGAEKSQMPERNLAVIEGFVSRLVVLDYDSAAATHT
GQIRAELARQGRPVGPFDQMIAGHARSRGLIVVTNNTREFERVAGIRLEDWS

Nucleotide

• Length: 399 bp

>T162108 NZ_CP071528:c120518-119766 [Legionella lytica]
ATGGTTGATATTTGGCTCGAATCTGACGGAAAAAACCATATCAAGCCATTGATGGCAAAAATTTACCGATTAGTAGAATC
CCAGGAGCAAATAGCAACTTTGGGGTTAGTAAACAATGTCTATGAGCAAGGTGTATTAGAGGAATTATTGGAGTCAACTA
AAAACCCGTTGCCTAGTGATACAAGCACCCTACATTATTTACTGACAACACCATTTCGTTACCCACCATTGAGGTACGGC
TCTAGATTTGGAAGAACCTTTGAACAAGGAATTTTCTATGGCTCATTAAATGTAAATACAGCTTTATCAGAAACAGCATA
TTATCGATTTGTATACATGCTTGGTCCAGAAACACCGTACGAACAACCAATATCAAATCAATATACATCCTTTACCGTCA
AAGTGCGAAGCAGTAATAGTATTTTTTTAGATCAAGACCCTTTTTCACAATATGAAGTAATTTTGACCTCCCCTACGTCT
TATATAGAAACACAGCAGCTAGGCTCTACTATGCGTGAATCTGGTATAGAAATATTTCAATATATTTCCGCAAGAGATAA
GGAGAAGGGCAAAAATATAGCGCTATTTACTCCCAAGTCTTTTCAAAGCAAAACACCTGCGCAGATGACTACATGGCTGT
GTCAGACCAGTATCGATGAAGTAGGATTTATGGCAAAAGAAGAACAACAACGAACGTCCTATTCTCAAAAAGATTTCTGG
ATTAACAATACGTTCCCTTCCCCAGCGACCTAA

Antitoxin

Protein

• Length: 76 a.a.
• Molecular weight: 8498.61 Da
• Isoelectric Point: 4.3867

>AT162108 WP_000450524.1 NZ_CP055251:c3310120-3309893 [Escherichia coli]
METTVFLSNRSQAVRLPKAVALPEDVKKVEIIAIGRTRIITPAGESWDSWFDGENVSADFMDIRDQPAMQERESF

Nucleotide

• Length: 228 bp

>AT162108 NZ_CP071528:c120883-120518 [Legionella lytica]
ATGAATATTGAATCCAAACCAAGTGAAAGCGAGGTCTTAGCAATAGCTTTGCATAATTTAAAAGATATCTTAGATATTTC
AAATAATGATCTAAGTGAAATTATCGGGGTTCATCGCAACACCTTGTCGCGTTCATTAAGCAATAAGAAAATCGATGTTA
AAAGCAAGGAAGGAGAATGCTCCTTATTGCTAATAAGAATTTATCGTTCTTTGTTTGCATTAAATGGCGGCAATGTTGAT
GCAATGAAGCATTGGTTAAGAACCAATAATCGGCATATTCAGGGAATCCCTTTGGAAGCAATGAAAAGTGTTTTGGGATT
ATCTCGGGTTGTGAATTATTTAGATGCGATTCGAGGAAAGATTTAA

Structures

Antitoxin

• AlphaFold DB: A0A829CM33