Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) itaRT/DUF1778(antitoxin)
Location 1053848..1054685 Replicon chromosome
Accession NZ_CP055251
Organism Escherichia coli strain AH01

Toxin (Protein)


Gene name itaT Uniprot ID Q3Z4X7
Locus tag HUU83_RS04980 Protein ID WP_000227784.1
Coordinates 1053848..1054390 (-) Length 181 a.a.

Antitoxin (Protein)


Gene name itaR Uniprot ID I2UQS9
Locus tag HUU83_RS04985 Protein ID WP_001297137.1
Coordinates 1054374..1054685 (-) Length 104 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HUU83_RS04960 1049387..1050298 - 912 WP_000705874.1 2-dehydropantoate 2-reductase -
HUU83_RS04965 1050466..1050957 + 492 WP_001138904.1 nucleotide binding protein YajQ -
HUU83_RS04970 1051085..1052449 - 1365 WP_001000960.1 MFS transporter -
HUU83_RS04975 1052857..1053792 + 936 WP_001368479.1 sel1 repeat family protein -
HUU83_RS04980 1053848..1054390 - 543 WP_000227784.1 GNAT family N-acetyltransferase Toxin
HUU83_RS04985 1054374..1054685 - 312 WP_001297137.1 DUF1778 domain-containing protein Antitoxin
HUU83_RS04990 1054870..1055760 - 891 WP_000971336.1 heme o synthase -
HUU83_RS04995 1055772..1056101 - 330 WP_000019869.1 cytochrome o ubiquinol oxidase subunit IV -
HUU83_RS05000 1056101..1056715 - 615 WP_000179819.1 cytochrome o ubiquinol oxidase subunit III -
HUU83_RS05005 1056705..1058696 - 1992 WP_000467180.1 cytochrome o ubiquinol oxidase subunit I -
HUU83_RS05010 1058718..1059665 - 948 WP_001239441.1 cytochrome o ubiquinol oxidase subunit II -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(68-156)

Antitoxin

Vibrio phage ICP1, Orf50

(14-97)


Sequences

Toxin        


Download         Length: 181 a.a.        Molecular weight: 19805.02 Da        Isoelectric Point: 8.3395

>T162091 WP_000227784.1 NZ_CP055251:c1054390-1053848 [Escherichia coli]
MVDKHEEITLPIVLSCNYQSDITYPGQKQFDCGNPVIDKFVRASLKKSVRNSDCAAKALIDRQSGELIGICTFTAYSLEK
QRVSGVLQGSQPSEIGVVRLVMLGVARKYQKRGFGQDLLCDFFEHVKIIHQALPIKGVYLDADPAAINFYARLGFVQLSA
TPNAFGAVPMFLAIQHILAA

Download         Length: 543 bp

>T162091 NZ_CP071522:c2375362-2375084 [Escherichia coli]
ATGATAATGAATTTCAGACACAAGGGATTGCGTGACTTGTTTCTTCTCGGCAAAACCTCCGGCGTTATACCGACGCAAGT
CAAACGATTACGCCACCGACTCGCTGTGATTGATGCAGCATGCTGTCTTGCTGATATCGATATGCCCGGTTACCGACTAC
ATCCGTTAAGCGGCGATCGCGATGGAATTTGGGCGATATCTGTCTCGGGCAACTGGCGAATCACATTTGAATTCGTCAAT
GGCGATGCATACATACTGGATTACGAGGACTATCACTGA

Antitoxin


Download         Length: 104 a.a.        Molecular weight: 11358.04 Da        Isoelectric Point: 10.4825

>AT162091 WP_001297137.1 NZ_CP055251:c1054685-1054374 [Escherichia coli]
MPGKTATLPDVDKTLKNARVELKTSPDAKNKLREAAQAVGVDLSAFILSAAMERAESVLDNQRRRELSNQSWELMNQLIA
EPAQPTLALKALMKRKNSDGRQA

Download         Length: 312 bp

>AT162091 NZ_CP071522:c2375084-2374800 [Escherichia coli]
ATGAAAATGGCCAATCATCCCCGCCCGGGGGACATTATTCAGGAATCACTGGACGAACTTAATGTCAGCCTGCGCGAGTT
TGCCAGAGCAATGGAAATTGCGCCCTCAACGGCAAGCCGATTGCTGACCGGAAAAGCAGCTTTGACGCCAGAAATGGCAA
TAAAACTTTCCGTGGTGATCGGCAGTTCGCCGCAAATGTGGCTGAATCTGCAAAATGCCTGGAGTCTGGCAGAGGCGGAA
AAAACGGTGGATGTGTCGCGACTGCGCCGTCTGGTTACGCAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 7BYY

Antitoxin

Source ID Structure
AlphaFold DB A0A0E0Y6B3

References