Detailed information of TA system TA162088

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/RHH(antitoxin)
• Location: 120307..120851
• Replicon: plasmid pZY-2
• Accession: NZ_CP055249
• Organism: Citrobacter freundii strain ZY198

Toxin (Protein)

• Gene name: relE
• Uniprot ID: -
• Locus tag: GTH18_RS25225
• Protein ID: WP_089069034.1
• Coordinates: 120576..120851 (+)
• Length: 92 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: -
• Locus tag: GTH18_RS25220
• Protein ID: WP_089069033.1
• Coordinates: 120307..120576 (+)
• Length: 90 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GTH18_RS25195	115352..116590	-	1239	WP_089069028.1	efflux RND transporter periplasmic adaptor subunit	-
GTH18_RS25200	116592..117869	-	1278	WP_089069029.1	TolC family protein	-
GTH18_RS25205	117917..118309	-	393	WP_089069030.1	hypothetical protein	-
GTH18_RS25210	118401..118751	-	351	WP_089069031.1	DUF3147 family protein	-
GTH18_RS25215	119049..120029	-	981	WP_089069032.1	DNA-binding protein	-
GTH18_RS25220	120307..120576	+	270	WP_089069033.1	ribbon-helix-helix protein, CopG family	Antitoxin
GTH18_RS25225	120576..120851	+	276	WP_089069034.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
GTH18_RS25230	120854..122041	+	1188	WP_089069035.1	hypothetical protein	-
GTH18_RS25235	122047..122985	+	939	WP_089069036.1	site-specific integrase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	aac(3)-IId / aph(6)-Id / aph(3'')-Ib / sul2	-	1..143918	143918
	-	flank	IS/Tn	-	-	123089..126094	3005

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (3-90)

Antitoxin

• Ribbon-helix-helix protein, CopG (9-44)

Sequences

Toxin

Protein

• Length: 92 a.a.
• Molecular weight: 10757.47 Da
• Isoelectric Point: 9.5571

>T162088 WP_089069034.1 NZ_CP055249:120576-120851 [Citrobacter freundii]
MELKWTSKALSDIARLYEFLATDHPQAAARTVQSLTRAPTVLLINPQIGEQLFEFMPREVRRLLIGQYELRYELQGQTVY
LLRLWHTREAR

Nucleotide

• Length: 276 bp

>T162088 NZ_CP071522:1986130-1986381 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCACCGAT
CCTTATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGTTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACAGAAAAGGAC
ATTATTGAGTGA

Antitoxin

Protein

• Length: 90 a.a.
• Molecular weight: 9900.18 Da
• Isoelectric Point: 5.0760

>AT162088 WP_089069033.1 NZ_CP055249:120307-120576 [Citrobacter freundii]
MTQTATKVLTAHIPQPLAERLDQAAGRLDRSRGWIMKQALSAWLSQEEERERLTREALADVDAGQVIDHQAVLAWAESLS
SDTPLPVPR

Nucleotide

• Length: 270 bp

>AT162088 NZ_CP071522:1986378-1986632 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACACCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GCTTCTGGTCCCGACGCATTATAGAGGAACACCGTCTGGTATACGCGGTTACTGATGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA