Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 4811206..4811822 Replicon chromosome
Accession NZ_CP055247
Organism Citrobacter freundii strain ZY198

Toxin (Protein)


Gene name vapC Uniprot ID A0A0J1MQ96
Locus tag GTH18_RS23050 Protein ID WP_003028682.1
Coordinates 4811206..4811580 (-) Length 125 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID A0A6B5NTK4
Locus tag GTH18_RS23055 Protein ID WP_043018956.1
Coordinates 4811580..4811822 (-) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
GTH18_RS23035 4808709..4809611 + 903 WP_003847898.1 formate dehydrogenase O subunit beta -
GTH18_RS23040 4809608..4810243 + 636 WP_003028686.1 formate dehydrogenase cytochrome b556 subunit -
GTH18_RS23045 4810240..4811169 + 930 WP_003028685.1 formate dehydrogenase accessory protein FdhE -
GTH18_RS23050 4811206..4811580 - 375 WP_003028682.1 type II toxin-antitoxin system VapC family toxin Toxin
GTH18_RS23055 4811580..4811822 - 243 WP_043018956.1 ribbon-helix-helix domain-containing protein Antitoxin
GTH18_RS23060 4812028..4812936 + 909 WP_003847901.1 alpha/beta hydrolase -
GTH18_RS23065 4813087..4814028 - 942 WP_003825286.1 fatty acid biosynthesis protein FabY -
GTH18_RS23070 4814073..4814510 - 438 WP_003028671.1 D-tyrosyl-tRNA(Tyr) deacylase -
GTH18_RS23075 4814507..4815379 - 873 WP_003028669.1 virulence factor BrkB family protein -
GTH18_RS23080 4815373..4815972 - 600 WP_003028663.1 glucose-1-phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-112)

Antitoxin

Ribbon-helix-helix protein, CopG

(13-47)


Sequences

Toxin        


Download         Length: 125 a.a.        Molecular weight: 13674.89 Da        Isoelectric Point: 9.5622

>T162080 WP_003028682.1 NZ_CP055247:c4811580-4811206 [Citrobacter freundii]
MVKGSALFDTNILIDLFSGRNEAKLAIETWPPQNAISLITWMEVMVGAKKYHQEARTRVALGAFNVIGVSQDIAERSVSI
RQEYGMKLPDAIILATAQIHRLTLVTRNTKDFAGLSGVVTPYTL

Download         Length: 375 bp

>T162080 NZ_CP055247:c4811580-4811206 [Citrobacter freundii]
ATGGTCAAAGGATCCGCGCTTTTTGATACCAACATCCTCATCGATCTGTTTAGTGGGAGAAATGAAGCAAAACTAGCGAT
AGAAACATGGCCGCCGCAGAATGCAATCAGCCTCATTACATGGATGGAGGTGATGGTGGGTGCAAAAAAATACCATCAGG
AAGCCCGTACTCGTGTTGCGCTAGGTGCTTTTAACGTGATCGGCGTTTCACAGGATATTGCTGAACGAAGCGTGAGTATC
AGACAGGAATATGGGATGAAATTGCCGGATGCCATTATTTTGGCAACAGCACAGATTCATCGTCTTACGCTGGTTACGCG
AAATACTAAGGATTTTGCAGGCCTATCCGGTGTGGTCACACCGTATACATTATAA

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9451.75 Da        Isoelectric Point: 4.3437

>AT162080 WP_043018956.1 NZ_CP055247:c4811822-4811580 [Citrobacter freundii]
MMAGMDMGRILLDLSDEVIKRLDDLKVQRNLPRAELLREAVEQYLERQDQAETTISRALGLWQGCEEDGVEYQRKLREEW

Download         Length: 243 bp

>AT162080 NZ_CP055247:c4811822-4811580 [Citrobacter freundii]
ATGATGGCTGGGATGGATATGGGCAGAATTTTACTCGATTTATCAGATGAAGTGATTAAACGTCTTGATGATCTCAAGGT
GCAGCGCAACCTGCCGCGAGCAGAACTTTTACGGGAGGCCGTTGAACAGTATCTTGAGAGGCAGGATCAGGCTGAAACCA
CAATTTCACGTGCTCTTGGGCTGTGGCAAGGCTGTGAGGAAGATGGTGTTGAATACCAGCGTAAACTCCGTGAGGAATGG
TAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0J1MQ96

Antitoxin

Source ID Structure
AlphaFold DB A0A6B5NTK4

References