Detailed information of TA system TA162079

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tad-ata/COG5606-COG4679
• Location: 4659684..4660350
• Replicon: chromosome
• Accession: NZ_CP055247
• Organism: Citrobacter freundii strain ZY198

Toxin (Protein)

• Gene name: tad
• Uniprot ID: -
• Locus tag: GTH18_RS22370
• Protein ID: WP_048216973.1
• Coordinates: 4659991..4660350 (-)
• Length: 120 a.a.

Antitoxin (Protein)

• Gene name: ata
• Uniprot ID: A0A0J1QJH0
• Locus tag: GTH18_RS22365
• Protein ID: WP_032938223.1
• Coordinates: 4659684..4660001 (-)
• Length: 106 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GTH18_RS22330	4654863..4655660	+	798	WP_003841402.1	PTS mannose/fructose/sorbose transporter subunit IIC	-
GTH18_RS22335	4655672..4656496	+	825	WP_048216971.1	PTS system mannose/fructose/sorbose family transporter subunit IID	-
GTH18_RS22340	4656564..4657787	+	1224	WP_003031639.1	L-sorbose 1-phosphate reductase	-
GTH18_RS22345	4657789..4658601	+	813	WP_048216972.1	shikimate 5-dehydrogenase	-
GTH18_RS22350	4658651..4659007	-	357	WP_032938220.1	hypothetical protein	-
GTH18_RS22355	4659139..4659312	+	174	WP_032938222.1	hypothetical protein	-
GTH18_RS22360	4659383..4659544	+	162	WP_003841416.1	phage protein	-
GTH18_RS22365	4659684..4660001	-	318	WP_032938223.1	helix-turn-helix domain-containing protein	Antitoxin
GTH18_RS22370	4659991..4660350	-	360	WP_048216973.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
GTH18_RS22375	4660564..4661253	+	690	WP_003841421.1	dipeptidase PepE	-
GTH18_RS22380	4661319..4662950	-	1632	WP_176350231.1	Na/Pi cotransporter family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (13-106)

Antitoxin

• HigA2-like, helix-turn-helix domain (12-90)

Sequences

Toxin

Protein

• Length: 120 a.a.
• Molecular weight: 13410.46 Da
• Isoelectric Point: 10.2555

>T162079 WP_048216973.1 NZ_CP055247:c4660350-4659991 [Citrobacter freundii]
MTKPLYWVGQARKDLLAMPEHVRDTFGFALWLAQQGKQHSQTKPLKGFGGAGVLEVVEDYHGSAWRAVYTIQLKNAVYVL
HVFQKKSVSGKATPKPEIDLIYQRLKAAQRHAQESGYVT

Nucleotide

• Length: 360 bp

>T162079 NZ_CP071521:3993771-3994112 [Escherichia coli]
ATGACTGACACGCATTCTATTGCACAACCGTTCGAAGCAGAAGTCTCCCCGGCAAATAACCGTCATGTCACCGTCGGTTA
TGCGAGTCGCTACCCGGATTACAGCCGTATTCCCGCCATCACCCTGAAAGGTCAGTGGCTGGAAGCCGCCGGTTTTGCCA
CTGGCACGGCGGTAGATGTCAAAGTGATGGAAGGCTGTATTGTCCTCACCGCCCAACCACCCGCCGCCGAGGAGAGCGAA
CTGATGCAGTCGCTACGCCAGGTGTGCAAACTGTCGGCGCGTAAACAAAAGCAGGTGCAGGCGTTTATTGGAGTGATTGC
CGGTAAACAGAAAGTCGCGTAA

Antitoxin

Protein

• Length: 106 a.a.
• Molecular weight: 11451.29 Da
• Isoelectric Point: 6.9796

>AT162079 WP_032938223.1 NZ_CP055247:c4660001-4659684 [Citrobacter freundii]
MSHEVEASSGNVYADLGAYDAEQMQLKAQLAMVIGEILQRKGLTQQQAAQLLGMTQPKLSNLLRGQFRGISEAKMLECLV
RLGRDVEIVVGEEGNMSGKLKVKFG

Nucleotide

• Length: 318 bp

>AT162079 NZ_CP071521:c3993776-3993700 [Escherichia coli]
AGTCATAACTGCTATTCTCCAGGAATAGTGATTGTGATTAGCGATGCGGGTGTGTTGGCGCACATCCGCACCGCGCT

Structures

Antitoxin

• AlphaFold DB: A0A0J1QJH0