Detailed information of TA system TA162077

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/ParE-RelB
• Location: 4361664..4362180
• Replicon: chromosome
• Accession: NZ_CP055247
• Organism: Citrobacter freundii strain ZY198

Toxin (Protein)

• Gene name: relE
• Uniprot ID: -
• Locus tag: GTH18_RS20940
• Protein ID: WP_048216920.1
• Coordinates: 4361664..4361948 (-)
• Length: 95 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: A0A0J1MV10
• Locus tag: GTH18_RS20945
• Protein ID: WP_003839576.1
• Coordinates: 4361938..4362180 (-)
• Length: 81 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GTH18_RS20925	4357989..4358573	+	585	WP_003839584.1	fructose PTS transporter subunit IIA	-
GTH18_RS20930	4358951..4361089	+	2139	WP_003839582.1	anaerobic ribonucleoside-triphosphate reductase	-
GTH18_RS20935	4361196..4361660	+	465	WP_032937736.1	anaerobic ribonucleoside-triphosphate reductase-activating protein	-
GTH18_RS20940	4361664..4361948	-	285	WP_048216920.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
GTH18_RS20945	4361938..4362180	-	243	WP_003839576.1	type II toxin-antitoxin system RelB/DinJ family antitoxin	Antitoxin
GTH18_RS20950	4362258..4364171	-	1914	WP_048216921.1	BglG family transcription antiterminator	-
GTH18_RS20955	4364193..4364933	-	741	WP_048216922.1	KDGP aldolase family protein	-
GTH18_RS20960	4364930..4366048	-	1119	WP_048216923.1	DgaE family pyridoxal phosphate-dependent ammonia lyase	-
GTH18_RS20965	4366032..4367165	-	1134	WP_048216924.1	amidohydrolase/deacetylase family metallohydrolase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (5-82)

Antitoxin

• RelB antitoxin/Antitoxin DinJ (1-69)

Sequences

Toxin

Protein

• Length: 95 a.a.
• Molecular weight: 10905.80 Da
• Isoelectric Point: 10.1715

>T162077 WP_048216920.1 NZ_CP055247:c4361948-4361664 [Citrobacter freundii]
MTYELEFDPRALKEWHKLGDKVKAQFKKKLADVLLNPRIDSARLNGLPDCYKIKLKSSGYRLVYQVRDEVVIVFVVAVGK
REHSAVYLDANKRL

Nucleotide

• Length: 285 bp

>T162077 NZ_CP071521:c3679659-3679261 [Escherichia coli]
ATGCGGGTATTCAAAACAAAACTTATTCGCCTGCAACTTACAGCAGAGGAACTTGATGCGTTAACGGCGGATTTTATTTC
CTATAAGCGTGACGGTGTTTTGCCAGATATATTTGGTCGCGATGCACTCTACGACGACTCCTTTACCTGGCCATTAATCA
AATTTGAGCGAGTTGCTCATATTCATCTGGCAAATGAGAATAATCCATTTCCGCCACAGTTGCGCCAATTCAGCAGAACG
AATGACGAAGCGCATTTGGTATATTGTCAGGGGGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGA
ACCTCATAAACTGGCTCGAGATAACAACCAAATGCATAAAATTGGGAAAATGGCAGAAGCGTTTCGCATGCGTTTTTGA

Antitoxin

Protein

• Length: 81 a.a.
• Molecular weight: 8961.34 Da
• Isoelectric Point: 5.0651

>AT162077 WP_003839576.1 NZ_CP055247:c4362180-4361938 [Citrobacter freundii]
MATLNVRLDDKLKNEAYAVLEKLNITPTEAVRLLFQYVAETGRMPVKTVTVSDSEDTLIQTVRERLASPQKGIKVSLDDL

Nucleotide

• Length: 243 bp

>AT162077 NZ_CP071521:c3679955-3679662 [Escherichia coli]
ATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGATCAACC
GGTTGCGGTTCTTTCTAATAATCGCCCCGCAGGATATCTCTTAAGTGCCAGCGCATTCGAAGCGTTAATGGACATGCTTG
CTGAACAAGAGGAGAAAAAGCCCATAAAGGCGCGCTTCCGTCCAAGTGCTGCAAGATTAGAGGAAATTACACGCCGCGCT
GAACAATATCTTAATGATATGACGGATGATGATTTCAATGACTTTAAGGAATAA

Structures

Antitoxin

• AlphaFold DB: A0A0J1MV10