Detailed information of TA system TA162076

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): ykfI-yfjZ/CbtA-CbeA
• Location: 4290569..4291272
• Replicon: chromosome
• Accession: NZ_CP055247
• Organism: Citrobacter freundii strain ZY198

Toxin (Protein)

• Gene name: ykfI
• Uniprot ID: A0A853JWF2
• Locus tag: GTH18_RS20615
• Protein ID: WP_032267594.1
• Coordinates: 4290569..4290910 (-)
• Length: 114 a.a.

Antitoxin (Protein)

• Gene name: yfjZ
• Uniprot ID: -
• Locus tag: GTH18_RS20620
• Protein ID: WP_032267593.1
• Coordinates: 4290931..4291272 (-)
• Length: 114 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GTH18_RS20595	4287073..4287435	+	363	WP_032937671.1	endoribonuclease SymE	-
GTH18_RS20600	4287482..4288117	-	636	WP_003837147.1	HNH endonuclease	-
GTH18_RS20605	4288258..4289130	+	873	WP_048216872.1	HNH endonuclease	-
GTH18_RS20610	4289311..4290318	-	1008	WP_032267595.1	restriction endonuclease	-
GTH18_RS20615	4290569..4290910	-	342	WP_032267594.1	TA system toxin CbtA family protein	Toxin
GTH18_RS20620	4290931..4291272	-	342	WP_032267593.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
GTH18_RS20625	4291283..4291825	-	543	WP_032267592.1	DNA repair protein RadC	-
GTH18_RS20630	4291838..4292281	-	444	WP_032267591.1	antirestriction protein	-
GTH18_RS20635	4292312..4293133	-	822	WP_032267590.1	DUF945 domain-containing protein	-
GTH18_RS20640	4293254..4293727	-	474	WP_032279719.1	hypothetical protein	-
GTH18_RS20645	4293822..4294361	-	540	WP_032267588.1	DUF4339 domain-containing protein	-
GTH18_RS20650	4294428..4294888	-	461	Protein_4044	hypothetical protein	-
GTH18_RS20655	4294885..4295340	-	456	WP_057688286.1	hypothetical protein	-
GTH18_RS20660	4295379..4296014	-	636	WP_032267586.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-107)

Antitoxin

• Antitoxin CbeA (13-110)

Sequences

Toxin

Protein

• Length: 114 a.a.
• Molecular weight: 12767.68 Da
• Isoelectric Point: 6.8699

>T162076 WP_032267594.1 NZ_CP055247:c4290910-4290569 [Citrobacter freundii]
MKTLPATTPQAATLCLSPVAVWQMLLARLLEQHYGLTLNDTPFSDEKVIQEHIDAGISLADAVNFLVEKYGLVRIDRRGF
GWQEQSPYLRAVDILRARQATGLLKARHGEHWE

Nucleotide

• Length: 342 bp

>T162076 NZ_CP071521:3669067-3669408 [Escherichia coli]
ATGAAAACTTTACCTGCAATAACTCAGCGGGCGGTGAAGCCCTGCCTGTCACCCGTGGCTGTCTGGCAAATGTTACTGAC
ACGTCTGCTGGAACAGCACTATGGTCTGACAATAAACGACACGCCATTCTGCAATGAGGCTGTGATTAAGGAACACATCG
ATGCCGGTATCACCCTAGCCGATGCCGTGAATTTTCTGGTAGAAAAATACGAGCTGGTTCGTATCGACAGGAAGGGATTT
AGCTGGCAGGAACAATCTCCTTATCTCCGGGCTGCAGACATTCTGCGAGCGCGGCAGGCAACTGGCTTGTTGCGGCAAAG
CCGTAACAACGTAGTACGATGA

Antitoxin

Protein

• Length: 114 a.a.
• Molecular weight: 12760.46 Da
• Isoelectric Point: 6.6213

>AT162076 WP_032267593.1 NZ_CP055247:c4291272-4290931 [Citrobacter freundii]
MKSTDSENDSFLKWGLNRNVTPCFGARLVQEGNRLHYLADRASITGKFSEAECLKLDLAFPHFISQMESMLTTGEINPRH
AHCVTLYHNGFTCEADTLGSCGYVYIAVYLTYR

Nucleotide

• Length: 342 bp

>AT162076 NZ_CP071521:3668729-3669046 [Escherichia coli]
ATGAGCAACCCTACCAGGGGCCTGCAGCGGGAGATTACACTGCGCCTGGGAGCCCGTCTGGTGCAGGAAGGCAACCGACT
GCATTATCTGGCTGACCGGGCCAGCATCACCGGCAAGTTCAGTGACATCGAATGCCGGAAGCTGGATGAAACATTCCCGC
ACTTTATCCTCCAGATGGAATCGATGCTGACCACCGGTGAACTCAGCCCCCACCATGCCCACTGCGTTACCCTGTACCAC
AACGATTTAACCTGCGAAGCCGACACCCTTGGCAGTTGCGGCTACGTATACATCGCCATTTACCCCACTCAGCGTTAA

Structures

Toxin

• AlphaFold DB: A0A853JWF2