162075

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) yafW-ykfI/CbtA-CbeA
Location 3913130..3913809 Replicon chromosome
Accession NZ_CP055247
Organism Citrobacter freundii strain ZY198

Toxin (Protein)


Gene name ykfI Uniprot ID A0A1B7JLK7
Locus tag GTH18_RS18905 Protein ID WP_003031349.1
Coordinates 3913468..3913809 (+) Length 114 a.a.

Antitoxin (Protein)


Gene name yafW Uniprot ID A0A1B7JLJ2
Locus tag GTH18_RS18900 Protein ID WP_003031347.1
Coordinates 3913130..3913447 (+) Length 106 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
GTH18_RS18875 3909223..3911220 - 1998 WP_016245729.1 choline BCCT transporter BetT -
GTH18_RS18880 3911724..3911915 + 192 Protein_3700 DUF905 family protein -
GTH18_RS18885 3911929..3912390 + 462 WP_003031344.1 antirestriction protein -
GTH18_RS18890 3912406..3912882 + 477 WP_003031345.1 RadC family protein -
GTH18_RS18895 3912891..3913112 + 222 WP_003031346.1 DUF987 domain-containing protein -
GTH18_RS18900 3913130..3913447 + 318 WP_003031347.1 type IV toxin-antitoxin system YeeU family antitoxin Antitoxin
GTH18_RS18905 3913468..3913809 + 342 WP_003031349.1 type IV toxin-antitoxin system toxin YkfI Toxin
GTH18_RS18915 3914439..3914903 - 465 WP_048216807.1 isoprenylcysteine carboxylmethyltransferase family protein -
GTH18_RS18920 3915096..3916544 - 1449 WP_048217399.1 EAL domain-containing protein -
GTH18_RS18925 3916886..3917359 - 474 WP_003838775.1 hypothetical protein -
GTH18_RS18930 3917334..3918065 - 732 WP_176350211.1 winged helix-turn-helix domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - - 3898771..3925706 26935


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

CbtA toxin of type IV toxin-antitoxin system

(1-112)

Antitoxin

Antitoxin CbeA

(5-104)


Sequences

Toxin        


Download         Length: 114 a.a.        Molecular weight: 12921.01 Da        Isoelectric Point: 9.6543

>T162075 WP_003031349.1 NZ_CP055247:3913468-3913809 [Citrobacter freundii]
MKTLPATTQRAVKPCLSPVAVWQMLLTRLLEQHYGLTINDTPFCNEAVIKEHIDAGITLADAVNFLVEKYELVRIDRKGF
SWQEQSPYLRAVDILRARQATGLLRQSRNNVVR

Download         Length: 342 bp

>T162075 NZ_CP071521:3452428-3452646 [Escherichia coli]
ATGTCCGAAAAATTTTTAACGAAAACCGATTATTTAATGCGTTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGTGT
TATCGAGAAAAATAAATACGAATTATCAGATAATGAACTGGCGGTATTTTACTCAGCCGCAGATCACCGCCTCGCCGAAT
TGACCATGAATAAACTGTACGACAAGATCCCTTCCTCAGTATGGAAATTTATTCGCTAA

Antitoxin


Download         Length: 106 a.a.        Molecular weight: 12094.74 Da        Isoelectric Point: 7.0370

>AT162075 WP_003031347.1 NZ_CP055247:3913130-3913447 [Citrobacter freundii]
MSKITWGLQRDITPRLGARLVQEGNRLHYLADRASMTGKFSDIEYRKLDETFPHFIRQMESMLTTGELSTHHAHCVTLYH
NDLTCEADTLGSCGYVYIAIYPTQR

Download         Length: 318 bp

>AT162075 NZ_CP071521:3452028-3452402 [Escherichia coli]
ATGGATGAATACTCACCCAAAAGACATGATATCGCACAGCTTAAGTTTCTCTGTGAAACCCTGTATCATGACTGCCTTGC
AAACCTTGAAGAAAGCAATCATGGCTGGGTAAACGACCCAACCTCGGCGATCAACCTCCAGTTGAATGAACTGATTGAGC
ATATTGCGACCTTCGCACTTAATTACAAAATTAAGTATAATGAAGACAATAAGCTCATTGAGCAGATCGACGAATATCTG
GATGACACCTTTATGTTGTTCAGTAGTTATGGTATTAATATGCAGGATCTTCAGAAATGGCGGAAGTCAGGTAATCGACT
ATTCCGTTGTTTTGTCAATGCGACGAAAGAGAATCCTGCGAGTTTATCTTGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A1B7JLK7

Antitoxin

Source ID Structure
AlphaFold DB A0A1B7JLJ2

References