162061

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hipBA/HipA-HipB
Location 691775..693398 Replicon chromosome
Accession NZ_CP055247
Organism Citrobacter freundii strain ZY198

Toxin (Protein)


Gene name hipA Uniprot ID -
Locus tag GTH18_RS03375 Protein ID WP_103798313.1
Coordinates 692073..693398 (+) Length 442 a.a.

Antitoxin (Protein)


Gene name hipB Uniprot ID -
Locus tag GTH18_RS03370 Protein ID WP_148362656.1
Coordinates 691775..692068 (+) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
GTH18_RS03345 687100..690108 - 3009 WP_176350077.1 Tn3 family transposase -
GTH18_RS03350 690267..690395 + 129 Protein_657 recombinase family protein -
GTH18_RS03355 690455..690703 + 249 Protein_658 transposase -
GTH18_RS03360 690768..691097 + 330 Protein_659 IS30 family transposase -
GTH18_RS03365 691096..691374 + 279 Protein_660 hypothetical protein -
GTH18_RS03370 691775..692068 + 294 WP_148362656.1 helix-turn-helix transcriptional regulator Antitoxin
GTH18_RS03375 692073..693398 + 1326 WP_103798313.1 type II toxin-antitoxin system HipA family toxin Toxin
GTH18_RS03380 693459..693665 + 207 WP_000134171.1 hypothetical protein -
GTH18_RS03385 693767..694177 + 411 WP_000985911.1 hypothetical protein -
GTH18_RS03390 694190..695005 + 816 WP_001572439.1 HNH endonuclease -
GTH18_RS03395 695088..695867 + 780 Protein_666 IS5-like element ISKpn26 family transposase -
GTH18_RS03400 695951..696391 + 441 WP_004189163.1 IS66 family insertion sequence hypothetical protein -
GTH18_RS03405 696388..696738 + 351 WP_004189161.1 IS66 family insertion sequence element accessory protein TnpB -
GTH18_RS03410 696769..698361 + 1593 WP_004902302.1 IS66-like element ISKox1 family transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside IScluster/Tn - - 687100..698361 11261


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HipA-like, C-terminal

(155-404)

Antitoxin

Cro/C1-type helix-turn-helix domain

(20-70)


Sequences

Toxin        


Download         Length: 442 a.a.        Molecular weight: 50333.27 Da        Isoelectric Point: 5.8810

>T162061 WP_103798313.1 NZ_CP055247:692073-693398 [Citrobacter freundii]
MSRKQQRLVIWMNGIKVGYWEKSKGVDSLEYLPEWVADEQGRPLSLSLPFTPGNQVWRGNVVRDYFDNLLPDSEGIRRRL
AMRYKADSLEPFDLLTELGKDCVGAIQLLHDGDEPTDLYSVKYHPLTESEIAATLRNTTETLLPGRPEDNDDLRLSIAGA
QEKTALLWYEDRWCMPEGNTPTTHIFKLPLGLVGNMKADMSSSVENEWLCSVLLDQYGLPVARTQIAHFEDQKALVVERF
DRKWSGDGQWIIRLPQEDMCQALGVSPLRKYQADGGPGISEIMEVLSNSDRAERDKAQFFMTQIIFWMMAATDGHAKNFS
ISIGPQGRYHLTPNYDVLSAWPVIGHGNNQISWQKCKLAMAVRGSSNYYQIYRIQRRHWIRHGEITGLSKQQTEAMIEEI
IARTPGVIERVSGLLPDQFPQQLAESIFDGMRQQCRRLAEK

Download         Length: 1326 bp

>T162061 NZ_CP071521:842915-843322 [Escherichia coli]
GTGGTCCTGTGGCAATCTGATTTGCGCGTCTCCTGGCGCGCACAGTGGCTTTCCTTGCTGATTCATGGGCTGGTTGCCGC
TGTTATTTTACTCATGCCCTGGCCACTCAGTTACACCCCGTTATGGATGGTGTTACTTTCGCTGGTGGTGTTTGATTGCG
TTCGCAGCCAGCGGCGTATTAATGCTCGCCAGGGGGAAATTCGCTTGTTGATGGACGGGCGTTTGCGTTGGCAAGGGCAG
GAGTGGAGCATCGTCAAAGCACCGTGGATGATTAAGAGCGGCATGATGCTGCGTTTACGTTCTGATGGCGGTAAACGGCA
ACATTTATGGCTGGCAGCCGACAGCATGGACGAAGCTGAATGGCGGGATTTACGGCGGATTTTGTTGCAACAAGAGACGC
AAAGATAA

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 11016.43 Da        Isoelectric Point: 9.9492

>AT162061 WP_148362656.1 NZ_CP055247:691775-692068 [Citrobacter freundii]
INNDYPLNTLNQLRPLLIGFRKANGLTQKDLSERLGVTQQTYSRLEANPASASIERLFKVFSVLGVKISFSSPTTSSERK
QTEEIYKLNSPARQEDW

Download         Length: 294 bp

>AT162061 NZ_CP071521:842668-842934 [Escherichia coli]
ATGGACATTAACAACAAAGCCCGCATTCATTGGGCATGCCGCCGTGGTATGCGCGAACTCGATATTTCAATCATGCCGTT
TTTCGAACATGAGTACGACAGCTTAAGCGATGACGAAAAACGCATCTTTATTCGTCTGCTGGAATGTGACGATCCGGACC
TGTTTAACTGGCTGATGAATCACGGTAAACCGGCCGATGCAGAACTGGAAATGATGGTCCGACTCATCCAGACACGGAAC
CGGGAACGTGGTCCTGTGGCAATCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure

Antitoxin

Source ID Structure

References