Detailed information of TA system TA162060

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): phd-doc/Doc-RelB
• Location: 276740..277326
• Replicon: chromosome
• Accession: NZ_CP055247
• Organism: Citrobacter freundii strain ZY198

Toxin (Protein)

• Gene name: doc
• Uniprot ID: A0A0J1QS94
• Locus tag: GTH18_RS01305
• Protein ID: WP_032937237.1
• Coordinates: 276958..277326 (+)
• Length: 123 a.a.

Antitoxin (Protein)

• Gene name: phd
• Uniprot ID: A0A0J1N092
• Locus tag: GTH18_RS01300
• Protein ID: WP_032937236.1
• Coordinates: 276740..276961 (+)
• Length: 74 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GTH18_RS01275	272539..273465	+	927	WP_003023435.1	high-affinity branched-chain amino acid ABC transporter permease LivH	-
GTH18_RS01280	273462..274739	+	1278	WP_032950432.1	branched chain amino acid ABC transporter permease LivM	-
GTH18_RS01285	274736..275503	+	768	WP_003023438.1	high-affinity branched-chain amino acid ABC transporter ATP-binding protein LivG	-
GTH18_RS01290	275521..276234	+	714	WP_003827581.1	high-affinity branched-chain amino acid ABC transporter ATP-binding protein LivF	-
GTH18_RS01295	276337..276618	+	282	WP_003023442.1	hypothetical protein	-
GTH18_RS01300	276740..276961	+	222	WP_032937236.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
GTH18_RS01305	276958..277326	+	369	WP_032937237.1	type II toxin-antitoxin system death-on-curing family toxin	Toxin
GTH18_RS01310	277570..278886	+	1317	WP_003023445.1	sn-glycerol-3-phosphate ABC transporter substrate-binding protein UgpB	-
GTH18_RS01315	278987..279874	+	888	WP_032937238.1	sn-glycerol-3-phosphate ABC transporter permease UgpA	-
GTH18_RS01320	279871..280716	+	846	WP_003023450.1	sn-glycerol-3-phosphate ABC transporter permease UgpE	-
GTH18_RS01325	280719..281789	+	1071	WP_003837845.1	sn-glycerol-3-phosphate import ATP-binding protein UgpC	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	274736..282529	7793

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Death on curing protein (1-121)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-50)

Sequences

Toxin

Protein

• Length: 123 a.a.
• Molecular weight: 13617.91 Da
• Isoelectric Point: 6.9891

>T162060 WP_032937237.1 NZ_CP055247:276958-277326 [Citrobacter freundii]
MTLQIISAEEIIQFHDRLLRVTPGVAGMPDPGRAEALMYRVLNQIEYEGVTDVWLLAAMHLLAISRGHIFNDGNKRTALF
ITLLFLKRNGIVLSANPDFVAMTVEAAAGQLTLEQIAHRLRH

Nucleotide

• Length: 369 bp

>T162060 NZ_CP071521:715194-715490 [Escherichia coli]
ATGGAAAAACGCACACCACATACACGTTTGAGTCAGGTTAAAAAACTTGTCAATGCCGGGCAAGTTCGTACAACACGTAG
TGCCCTGTTAAATGCAGATGAGTTAGGTTTGGATTTTGATGGTATGTGTAATGTTATCATTGGATTATCAGAGAGCGACT
TTTATAAAAGCATGACCACCTACTCTGATCATACTATCTGGCAGGATGTTTACAGACCCAGGCTTGTTACAGGCCAGGTT
TATCTTAAAATTACGGTAATTCATGACGTACTGATCGTCTCGTTTAAGGAGAAGTAA

Antitoxin

Protein

• Length: 74 a.a.
• Molecular weight: 8306.41 Da
• Isoelectric Point: 4.7906

>AT162060 WP_032937236.1 NZ_CP055247:276740-276961 [Citrobacter freundii]
MRTMNYSEARQNLAEVLESAVTGVPVTITRRGHKPAVIISAEEFERYQAARLDDEFADIMMIHGNEIRELADK

Nucleotide

• Length: 222 bp

>AT162060 NZ_CP071521:715492-715887 [Escherichia coli]
ATGAAATGTCCGGTTTGCCACCAGGGAGAAATGGTTTCTGGCATTAAAGATATTCCATACACCTTCCGTGGACGAAAAAC
AGTATTGAAAGGTATCCACGGTTTATATTGTGTCCATTGCGAAGAGAGCATCATGAATAAAGAAGAGTCAGATGCTTTCA
TGGCGCAAGTAAAGGCATTTCGGGCTTCGGTGAATGCCGAAACAGTGGCACCTGAATTTATAGTGAAGGTTCGAAAAAAG
CTCTCTCTTACCCAAAAAGAGGCAAGCGAAATTTTTGGGGGAGGTGTAAATGCGTTTTCGCGTTACGAAAAAGGCAATGC
CCAACCTCATCCTTCCACAATCAAACTTTTACGTGTTCTGGATAAGCATCCAGAACTATTGAATGAAATCCGTTAA

Structures

Toxin

• AlphaFold DB: A0A0J1QS94

Antitoxin

• AlphaFold DB: A0A0J1N092